2. Immunology/Inflammation GPCR/G Protein PI3K/Akt/mTOR NF-κB
  3. NO Synthase Prostaglandin Receptor PI3K Akt NF-κB Keap1-Nrf2 COX
  4. trans-Isoferulic acid

trans-Isoferulic acid  (Synonyms: trans-3-Hydroxy-4-methoxycinnamic acid)

Cat. No.: HY-N0761A Purity: 99.93%
COA
SDS
Handling Instructions Technical Support

trans-Isoferulic acid (trans-3-Hydroxy-4-methoxycinnamic acid) is an aromatic acid that exhibits anti-inflammatory activity. trans-Isoferulic acid dephosphorylates PI3K/Akt, suppresses LPS (HY-D1056)-induced NF-κB activation, enhances Nrf2 activity, downregulates iNOS and COX-2 expression, induces HO-1 expression, inhibits NO and PGE2 production, and scavenges hydroperoxyl radical. trans-Isoferulic acid can be used for research of inflammatiory diseases.

For research use only. We do not sell to patients.

trans-Isoferulic acid

trans-Isoferulic acid Chemical Structure

CAS No. : 25522-33-2

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
250 mg In-stock
500 mg In-stock
1 g In-stock
5 g In-stock
10 g   Get quote  
50 g   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of trans-Isoferulic acid:

trans-Isoferulic acid Related Antibodies

Top Publications Citing Use of Products

View All NO Synthase Isoform Specific Products:

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nNOS iNOS eNOS

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DP EP FP IP TXA2/TP TXB2

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PI3Kα PI3Kβ PI3Kγ PI3Kδ PI3KC2α PI3KC2β PI3KC2γ Vps34 PI3K PI3KC3 p120γ

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Akt Akt1 Akt2 Akt3

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

View All COX Isoform Specific Products:

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COX COX-1 COX-2 COX-3

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

trans-Isoferulic acid (trans-3-Hydroxy-4-methoxycinnamic acid) is an aromatic acid that exhibits anti-inflammatory activity. trans-Isoferulic acid dephosphorylates PI3K/Akt, suppresses LPS (HY-D1056)-induced NF-κB activation, enhances Nrf2 activity, downregulates iNOS and COX-2 expression, induces HO-1 expression, inhibits NO and PGE2 production, and scavenges hydroperoxyl radical. trans-Isoferulic acid can be used for research of inflammatiory diseases[1][2][3].

IC50 & Target[1]

iNOS

 

COX-2

 

In Vitro

trans-Isoferulic acid (25-200 μM; 1 h pretreatment) does not reduce viability or induce apoptosis in BV2 microglial cells at concentrations ≤150 μM, but causes significant cytotoxicity at 200 μM[1].
trans-Isoferulic acid (50-150 μM; 1 h pretreatment) dose-dependently inhibits LPS-induced NO production in BV2 microglial cells, with complete inhibition at 150 μM[1].
trans-Isoferulic acid (50-150 μM; 1 h pretreatment) dose-dependently inhibits LPS-induced PGE2 production in BV2 microglial cells, with complete inhibition at 150 μM[1].
trans-Isoferulic acid (50-150 μM; 1 h pretreatment) dose-dependently suppresses LPS-induced iNOS and COX-2 mRNA and protein expression in BV2 microglial cells[1].
trans-Isoferulic acid (150 μM; 1 h pretreatment) completely inhibits LPS-induced NF-κB DNA-binding activity in BV2 microglial cells[1].
trans-Isoferulic acid (150 μM; 1 h pretreatment) suppresses LPS-induced phosphorylation of PI3K and Akt in BV2 microglial cells[1].
trans-Isoferulic acid (50-150 μM; 0-24 h) time- and dose-dependently upregulates HO-1 mRNA and protein expression in BV2 microglial cells[1].
trans-Isoferulic acid (50-150 μM; 1 h pretreatment) dose-dependently increases Nrf2 DNA-binding activity in BV2 microglial cells[1].
trans-Isoferulic acid (150 μM; 1 h pretreatment) induced HO-1 expression and subsequent inhibition of LPS-induced NO and PGE2 production in BV2 microglial cells are dependent on Nrf2 activation[1].
trans-isoferulic acid scavenges HOO• radicals via pH-dependent mechanisms[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

trans-Isoferulic acid Related Antibodies

Cell Viability Assay[1]

Cell Line: BV2 microglial cells
Concentration: 25; 50; 75; 100; 150; 200 μM
Incubation Time: 24 h (with 1 h pre-incubation before LPS)
Result: Showed no cytotoxicity at concentrations ≤150 μM, regardless of LPS treatment.
Detected a statistically significant reduction in cell viability at 200 μM.

ELISA Assay[1]

Cell Line: LPS-stimulated BV2 microglial cells
Concentration: 50; 100;150 μM
Incubation Time: 24 h (with 1 h pre-incubation before LPS)
Result: Reduced LPS-induced PGE2 levels in a dose-dependent manner.
Dose-dependently suppressed LPS-induced iNOS and COX-2 mRNA.

Real Time qPCR[1]

Cell Line: LPS-stimulated BV2 microglial cells
Concentration: 50; 100; 150 μM
Incubation Time: 3; 6; 9 h
Result: Significantly decreased LPS-induced HO-1 mRNA levels in a dose-dependent manner.
Significantly decreased LPS-induced HO-1 mRNA levels in a time-dependent manner.
Molecular Weight

194.19

Formula

C10H10O4
CAS No.
Appearance

Solid

Color

White to light brown

SMILES

O=C(O)/C=C/C1=CC=C(OC)C(O)=C1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (514.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.1496 mL 25.7480 mL 51.4960 mL
5 mM 1.0299 mL 5.1496 mL 10.2992 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (12.87 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (12.87 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.93%

SDS (394 KB)

COA (246 KB) HNMR (132 KB) LCMS (105 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.1496 mL 25.7480 mL 51.4960 mL 128.7399 mL
5 mM 1.0299 mL 5.1496 mL 10.2992 mL 25.7480 mL
10 mM 0.5150 mL 2.5748 mL 5.1496 mL 12.8740 mL
15 mM 0.3433 mL 1.7165 mL 3.4331 mL 8.5827 mL
20 mM 0.2575 mL 1.2874 mL 2.5748 mL 6.4370 mL
25 mM 0.2060 mL 1.0299 mL 2.0598 mL 5.1496 mL
30 mM 0.1717 mL 0.8583 mL 1.7165 mL 4.2913 mL
40 mM 0.1287 mL 0.6437 mL 1.2874 mL 3.2185 mL
50 mM 0.1030 mL 0.5150 mL 1.0299 mL 2.5748 mL
60 mM 0.0858 mL 0.4291 mL 0.8583 mL 2.1457 mL
80 mM 0.0644 mL 0.3218 mL 0.6437 mL 1.6092 mL
100 mM 0.0515 mL 0.2575 mL 0.5150 mL 1.2874 mL
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trans-Isoferulic acid Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

trans-Isoferulic acid25522-33-2trans-3-Hydroxy-4-methoxycinnamic acidNO SynthaseProstaglandin ReceptorPI3KAktNF-κBKeap1-Nrf2COXNitric oxide synthasesNOSPhosphoinositide 3-kinasePKBProtein kinase BNuclear factor-κBNuclear factor-kappaBCyclooxygenasePI3K/AktCOX-2Fe2+HO-1Nrf2BV2 microglial cellsmicroglial cellsiNOSUV radiationInhibitorinhibitorinhibit

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