ANO1-IN-1
Based on 2 publication(s) in Google Scholar
ANO1-IN-1 (Compound 9c) is a selective ANO1 channel blocker with an IC50 of 2.56 μM and 15.43 μM against ANO1 and ANO2, respectively. ANO1-IN-1 suppresses strongly proliferation of glioblastoma cells.
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- Pureza: 99.57%
- No. CAS: 407587-01-3
- Fòrmula: C18H28N2O2S
- Peso molecular:336.49
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) ANO1-IN-1
More
Actividad biológica
IC50: 2.56 μM (ANO1), 15.43 μM (ANO2)[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Astrocyte | GI50 |
94.83 μM
Compound: 9c
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Antiproliferative activity against mouse primary astrocytes assessed as reduction in cell viability measured after 72 hrs by Celltiter-Glo luminescent assay
Antiproliferative activity against mouse primary astrocytes assessed as reduction in cell viability measured after 72 hrs by Celltiter-Glo luminescent assay
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[PMID: 32906067] |
| U138-MG | GI50 |
22.24 μM
Compound: 9c
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Antiproliferative activity against human U138 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay
Antiproliferative activity against human U138 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay
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[PMID: 32906067] |
| U-251 | GI50 |
12.58 μM
Compound: 9c
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Antiproliferative activity against human U251 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay
Antiproliferative activity against human U251 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay
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[PMID: 32906067] |
| U-251 | GI50 |
5.32 μM
Compound: 9c
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Antiproliferative activity against human U251 cells overexpressing ANO1 assessed as reduction in cell viability in presence of temozolomide measured after 72 hrs by Celltiter-glo assay
Antiproliferative activity against human U251 cells overexpressing ANO1 assessed as reduction in cell viability in presence of temozolomide measured after 72 hrs by Celltiter-glo assay
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[PMID: 32906067] |
| U-87MG ATCC | GI50 |
31.49 μM
Compound: 9c
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Antiproliferative activity against human U87MG cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay
Antiproliferative activity against human U87MG cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay
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[PMID: 32906067] |
ANO1-IN-1 (Compound 9c) (10 μM) significantly suppresses migration and invasion of U251 cells[1].
ANO1-IN-1 and TMZ combination brings about remarkable synergistic effects in suppressing proliferation of GBM cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 407587-01-3
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Appearance Solid
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Peso molecular 336.49
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Fòrmula C18H28N2O2S
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Color White to off-white
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SMILES
CC(C)(C(NC1=C(C2=C(S1)CC(CC2)C(C)(C)C)C(N)=O)=O)C
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (2)
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Journal Impact Factor
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Most Recent
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Int J Mol Sci
LRRC8A Inhibition Overcomes Chemoresistance by Downregulating MRP3 and CYP3A4 in the 3D Spheroid Model of Human Breast Cancer Cells. [Abstract]2026 Mar 13;27(6):2646. PMID: 41898509 -
Int J Mol Sci
Downregulation of IL-8 and IL-10 by LRRC8A Inhibition through the NOX2-Nrf2-CEBPB Transcriptional Axis in THP-1-Derived M2 Macrophages. [Abstract]2024 Sep 5;25(17):9612. PMID: 39273558
Solvente y solubilidad
DMSO : 175 mg/mL (520.07 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (14.86 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9719 mL | 14.8593 mL | 29.7186 mL | 74.2964 mL |
| 5 mM | 0.5944 mL | 2.9719 mL | 5.9437 mL | 14.8593 mL | |
| 10 mM | 0.2972 mL | 1.4859 mL | 2.9719 mL | 7.4296 mL | |
| 15 mM | 0.1981 mL | 0.9906 mL | 1.9812 mL | 4.9531 mL | |
| 20 mM | 0.1486 mL | 0.7430 mL | 1.4859 mL | 3.7148 mL | |
| 25 mM | 0.1189 mL | 0.5944 mL | 1.1887 mL | 2.9719 mL | |
| 30 mM | 0.0991 mL | 0.4953 mL | 0.9906 mL | 2.4765 mL | |
| 40 mM | 0.0743 mL | 0.3715 mL | 0.7430 mL | 1.8574 mL | |
| 50 mM | 0.0594 mL | 0.2972 mL | 0.5944 mL | 1.4859 mL | |
| 60 mM | 0.0495 mL | 0.2477 mL | 0.4953 mL | 1.2383 mL | |
| 80 mM | 0.0371 mL | 0.1857 mL | 0.3715 mL | 0.9287 mL | |
| 100 mM | 0.0297 mL | 0.1486 mL | 0.2972 mL | 0.7430 mL |
ANO1-IN-1 Related Classifications
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