1. Membrane Transporter/Ion Channel
  2. Chloride Channel

NPPB (Synonyms: Hoechst 144; HOE 144)

Cat. No.: HY-101012 Purity: 99.89%
Handling Instructions

NPPB is a blocker of the outwardly rectifying chloride channel (ORCC).

For research use only. We do not sell to patients.
NPPB Chemical Structure

NPPB Chemical Structure

CAS No. : 107254-86-4

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 66 In-stock
10 mg USD 60 In-stock
50 mg USD 192 In-stock
100 mg USD 336 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Related Screening Libraries:

  • Biological Activity

  • Protocol

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  • Purity & Documentation

  • References


NPPB is a blocker of the outwardly rectifying chloride channel (ORCC).

IC50 & Target


In Vitro

0.1 mM NPPB in the bath solution reduces channel open probability from 0.89±0.06 to 0.11±0.04 (n=5, P<0.01)[1]. Dose-dependent inhibition of chloride currents is observed with a 50% inhibitory concentration (IC50) of 125 μM NPPB. NPPB itself also shows cytotoxicity against glioma cells with a GI50 of approximately 500 μM[2].

Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 3.3299 mL 16.6495 mL 33.2989 mL
5 mM 0.6660 mL 3.3299 mL 6.6598 mL
10 mM 0.3330 mL 1.6649 mL 3.3299 mL
Please refer to the solubility information to select the appropriate solvent.
Cell Assay

Cells are seeded in the 96-well microtiter plate at a density of 5×103 cells per well and incubated at 37°C for 24 h in a humidified 5% CO2 atmosphere. After removing the culture medium, fresh media containing various concentrations of NPPB is added, and incubated for 24 h. Next, 100 μL of Thiazolyl blue tetrazolium bromide at 0.5 mg/mL is added to each well and incubated at 37°C for 1 h. Cells are then dissolved in 100 μL of DMSO, and the absorbance is measured at 570 nm with a Microplate Reader. Concentration-response curves of NPPB are fitted to a Hill equation to obtain GI50 and GI80 (50% and 80% growth inhibition concentrations, respectively) values[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight








Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: 150 mg/mL

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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