Ferruginol (Synonyms: (+)-Ferruginol)

Name: Ferruginol
Brand: MedChemExpress
SKU: HY-N6033
Rating: 4.5 (0 reviews)

Cat. No.: HY-N6033 Purity: 96.91%: Data Sheet
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Ferruginol ((+)-Ferruginol), a natural diterpenoid, is an inhibitor of the activation of Epstein-Barr virus early antigen (EBV-EA). Ferruginol inhibits the growth of thyroid cancer cells through the induction of mitochondrial apoptosis. Ferruginol has antitumor, cardioprotective, antioxidant, gastroprotective, and neuroprotective activities.

For research use only. We do not sell to patients.

Ferruginol Chemical Structure

CAS No. : 514-62-5

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
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Biological Activity
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	ED₅₀	6.47 μg/mL Compound: ferruginol	Cytotoxicity against human A549 cells after 7 days by MTT assay Cytotoxicity against human A549 cells after 7 days by MTT assay	[PMID: 9014350]
A549	GI₅₀	<50 μg/mL Compound: 2	Inhibitory activity against human A549 lung tumor cell proliferation Inhibitory activity against human A549 lung tumor cell proliferation	[PMID: 15808460]
A549	GI₅₀	30.67 μM Compound: 14	Cytotoxicity against human A549 cells assessed as growth inhibition by WST-8 assay Cytotoxicity against human A549 cells assessed as growth inhibition by WST-8 assay	[PMID: 24210499]
A549	IC₅₀	>40 μM Compound: 25	Cytotoxicity against human A549 cells after 48 hrs by MTT assay Cytotoxicity against human A549 cells after 48 hrs by MTT assay	[PMID: 23691952]
AGS	IC₅₀	18 μM Compound: 3	Cytotoxicity against human AGS cells Cytotoxicity against human AGS cells	[PMID: 25440884]
BJ	EC₅₀	19.57 μM Compound: 1	Cytotoxicity against human BJ cells Cytotoxicity against human BJ cells	[PMID: 25316317]
BXPC-3	GI₅₀	21.14 μM Compound: 14	Cytotoxicity against human BxPC3 cells assessed as growth inhibition by WST-8 assay Cytotoxicity against human BxPC3 cells assessed as growth inhibition by WST-8 assay	[PMID: 24210499]
HCT-116	GI₅₀	<50 μg/mL Compound: 2	Growth inhibitory activity against human HCT116 colon tumor cell line Growth inhibitory activity against human HCT116 colon tumor cell line	[PMID: 15808460]
HEK293	EC₅₀	>25 μM Compound: 1	Cytotoxicity against HEK293 cells Cytotoxicity against HEK293 cells	[PMID: 25316317]
HeLa	IC₅₀	64.9 μM Compound: 1	Cytotoxicity against human HeLa cells assessed as cell growth inhibition after 48 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as cell growth inhibition after 48 hrs by MTT assay	[PMID: 26638041]
HepG2	EC₅₀	>25 μM Compound: 1	Cytotoxicity against human HepG2 cells Cytotoxicity against human HepG2 cells	[PMID: 25316317]
HepG2	IC₅₀	>40 μM Compound: 19	Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 20402524]
HL-60	GI₅₀	27.33 μM Compound: 14	Cytotoxicity against human HL60 cells assessed as growth inhibition by WST-8 assay Cytotoxicity against human HL60 cells assessed as growth inhibition by WST-8 assay	[PMID: 24210499]
HL-60	IC₅₀	>40 μM Compound: 19	Cytotoxicity against human HL60 cells after 48 hrs by MTT assay Cytotoxicity against human HL60 cells after 48 hrs by MTT assay	[PMID: 20402524]
HL-60	IC₅₀	>40 μM Compound: 25	Cytotoxicity against human HL60 cells after 48 hrs by MTT assay Cytotoxicity against human HL60 cells after 48 hrs by MTT assay	[PMID: 23691952]
HT-29	ED₅₀	6.43 μg/mL Compound: ferruginol	Cytotoxicity against human HT-29 cells after 7 days by MTT assay Cytotoxicity against human HT-29 cells after 7 days by MTT assay	[PMID: 9014350]
HT-29	IC₅₀	39.4 μM Compound: 12	Cytotoxicity against human HT-29 cells after 72 hrs by SRB assay Cytotoxicity against human HT-29 cells after 72 hrs by SRB assay	[PMID: 26905523]
Jurkat	IC₅₀	35.1 μM Compound: 19	Cytotoxicity against human Jurkat cells after 48 hrs by MTT assay Cytotoxicity against human Jurkat cells after 48 hrs by MTT assay	[PMID: 20402524]
Jurkat	IC₅₀	48.2 μM Compound: 1	Cytotoxicity against human Jurkat cells assessed as cell growth inhibition after 48 hrs by MTT assay Cytotoxicity against human Jurkat cells assessed as cell growth inhibition after 48 hrs by MTT assay	[PMID: 26638041]
MCF7	ED₅₀	23.42 μg/mL Compound: ferruginol	Cytotoxicity against human MCF7 cells after 7 days by MTT assay Cytotoxicity against human MCF7 cells after 7 days by MTT assay	[PMID: 9014350]
MCF7	IC₅₀	>40 μM Compound: 25	Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 23691952]
MDA-MB-231	GI₅₀	<50 μg/mL Compound: 2	Growth inhibitory activity against human MDA-MB-231 breast tumor cell line Growth inhibitory activity against human MDA-MB-231 breast tumor cell line	[PMID: 15808460]
MIA PaCa-2	IC₅₀	25.9 μM Compound: 20	Cytotoxicity against human MIAPaCa2 cells after 24 hrs by MTT assay Cytotoxicity against human MIAPaCa2 cells after 24 hrs by MTT assay	[PMID: 21775156]
MRC5	GI₅₀	40.01 μM Compound: 14	Cytotoxicity against human MRC5 cells assessed as growth inhibition by WST-8 assay Cytotoxicity against human MRC5 cells assessed as growth inhibition by WST-8 assay	[PMID: 24210499]
MRC5	IC₅₀	19 μM Compound: 3	Cytotoxicity against human MRC5 cells Cytotoxicity against human MRC5 cells	[PMID: 25440884]
NCI-H23	GI₅₀	<50 μg/mL Compound: 2	Growth inhibitory activity against human NCI-H23 lung tumor cell line Growth inhibitory activity against human NCI-H23 lung tumor cell line	[PMID: 15808460]
PANC-1	GI₅₀	41.13 μM Compound: 14	Cytotoxicity against human PANC1 cells assessed as growth inhibition by WST-8 assay Cytotoxicity against human PANC1 cells assessed as growth inhibition by WST-8 assay	[PMID: 24210499]
PC-3	GI₅₀	56.45 μM Compound: 14	Cytotoxicity against human PC3 cells assessed as growth inhibition by WST-8 assay Cytotoxicity against human PC3 cells assessed as growth inhibition by WST-8 assay	[PMID: 24210499]
Raji	EC₅₀	11.49 μM Compound: 1	Cytotoxicity against human Raji cells Cytotoxicity against human Raji cells	[PMID: 25316317]
SMMC-7721	IC₅₀	>40 μM Compound: 25	Cytotoxicity against human SMMC7721 cells after 48 hrs by MTT assay Cytotoxicity against human SMMC7721 cells after 48 hrs by MTT assay	[PMID: 23691952]
SW480	IC₅₀	>40 μM Compound: 25	Cytotoxicity against human SW480 cells after 48 hrs by MTT assay Cytotoxicity against human SW480 cells after 48 hrs by MTT assay	[PMID: 23691952]
SW-620	GI₅₀	<50 μg/mL Compound: 2	Growth inhibitory activity against human SW620 colon tumor cell line Growth inhibitory activity against human SW620 colon tumor cell line	[PMID: 15808460]
U-937	IC₅₀	19.7 μM Compound: 19	Cytotoxicity against human U937 cells after 48 hrs by MTT assay Cytotoxicity against human U937 cells after 48 hrs by MTT assay	[PMID: 20402524]
U-937	IC₅₀	21.3 μM Compound: 1	Cytotoxicity against human U937 cells assessed as cell growth inhibition after 48 hrs by MTT assay Cytotoxicity against human U937 cells assessed as cell growth inhibition after 48 hrs by MTT assay	[PMID: 26638041]
Vero	CC₅₀	80.4 μM Compound: 1	Cytotoxicity against Vero E6 cells by MTT assay Cytotoxicity against Vero E6 cells by MTT assay	[PMID: 17663539]
Vero	EC₅₀	1.39 μM Compound: 1	Antiviral activity against SARS coronavirus in Vero E6 cells assessed as inhibition of viral replication by ELISA Antiviral activity against SARS coronavirus in Vero E6 cells assessed as inhibition of viral replication by ELISA	[PMID: 17663539]
Vero	IC₅₀	90.4 μM Compound: 1	Cytotoxicity against African green monkey Vero cells assessed as cell growth inhibition after 48 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as cell growth inhibition after 48 hrs by MTT assay	[PMID: 26638041]

In Vitro

Ferruginol (0-160 μM; 24 hours) exerts potent antiproliferative action against thyroid cancer cells, and an IC₅₀ of 12 μM for the MDA-T32 cell line. The toxic effects of Ferruginol are less pronounced against normal cells^[1].
Ferruginol (0-24 μM; 24 hours) induces apoptotic cell death of MDA-T32 cells. Ferruginol increases Bax expression and decreases Bcl-2 expression dose-dependently^[1].
Ferruginol (0-24 μM; 24 hours) inhibits the MAPK and PI3K/AKT signaling pathway of MDA-T32 cells^[1].
Ferruginol (0-24 μM; 24 hours) also causes ROS mediated alterations in the MMP of MDA-T32 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	MDA-T32 cells
Concentration:	0-160 μM
Incubation Time:	24 hours
Result:	Exerted potent antiproliferative action against thyroid cancer cells.

Apoptosis Analysis^[1]

Cell Line:	MDA-T32 cells
Concentration:	0 μM, 6 μM, 12 μM, and 24 μM
Incubation Time:	24 hours
Result:	Induced apoptotic cell death of MDA-T32 cells

Western Blot Analysis^[1]

Cell Line:	MDA-T32 cells
Concentration:	0 μM, 6 μM, 12 μM, and 24 μM
Incubation Time:	24 hours
Result:	Blocked the MAPK and PI3K/AKT signaling pathway.

In Vivo

Ferruginol (20 mg/kg; p.o.; daily; for 4 weeks) exerts cardioprotection manifested as enhanced cardiac function and reduced structural damage and apoptosis. The transcriptome and other results revealed that Ferruginol facilitates PGC-1α-mediated mitochondrial biogenesis and fatty acid oxidation (MB and FAO) by increasing the expression of PGC-1α and concurrently promoting the expression of SIRT1-enhancing deacetylase SIRT1 deacetylating and activating PGC-1α^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male C57BL/6 mice (20 g, 8-10 weeks old) with Doxorubicin (DOX)-induced cardiotoxicity (DIC)^[3].
Dosage:	20 mg/kg
Administration:	Administered intragastrically; daily; for 4 weeks
Result:	Relieved Doxorubicin-induced cardiac structural and functional lesion.

Molecular Weight

286.45

Formula

C₂₀H₃₀O

CAS No.

514-62-5

Appearance

Solid

Color

White to off-white

SMILES

[H][C@]1(CCC2=C3)C(C)(C)CCC[C@]1(C)C2=CC(O)=C3C(C)C

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (349.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.4910 mL	17.4551 mL	34.9101 mL
5 mM	0.6982 mL	3.4910 mL	6.9820 mL
10 mM	0.3491 mL	1.7455 mL	3.4910 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 96.91%

Select Batch:

Data Sheet (279 KB) SDS (252 KB)

COA (249 KB) HNMR (237 KB) RP-HPLC (212 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Guoqing Luo, et al. Ferruginol Diterpenoid Selectively Inhibits Human Thyroid Cancer Growth by Inducing Mitochondrial Dependent Apoptosis, Endogenous Reactive Oxygen Species (ROS) Production, Mitochondrial Membrane Potential Loss and Suppression of Mitogen-Activated Protein Kinase (MAPK) and PI3K/AKT Signaling Pathways. Med Sci Monit. 2019 Apr 21;25:2935-2942. [Content Brief]

[2]. Weili Li, et al. Ferruginol Restores SIRT1-PGC-1α-Mediated Mitochondrial Biogenesis and Fatty Acid Oxidation for the Treatment of DOX-Induced Cardiotoxicity. Front Pharmacol. 2021 Nov 24;12:773834. [Content Brief]

[3]. Manabu Iwamoto, et al. Potential antitumor promoting diterpenoids from the stem bark of Thuja standishii. Planta Med. 2003 Jan;69(1):69-72. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.4910 mL	17.4551 mL	34.9101 mL	87.2753 mL
	5 mM	0.6982 mL	3.4910 mL	6.9820 mL	17.4551 mL
	10 mM	0.3491 mL	1.7455 mL	3.4910 mL	8.7275 mL
	15 mM	0.2327 mL	1.1637 mL	2.3273 mL	5.8184 mL
	20 mM	0.1746 mL	0.8728 mL	1.7455 mL	4.3638 mL
	25 mM	0.1396 mL	0.6982 mL	1.3964 mL	3.4910 mL
	30 mM	0.1164 mL	0.5818 mL	1.1637 mL	2.9092 mL
	40 mM	0.0873 mL	0.4364 mL	0.8728 mL	2.1819 mL
	50 mM	0.0698 mL	0.3491 mL	0.6982 mL	1.7455 mL
	60 mM	0.0582 mL	0.2909 mL	0.5818 mL	1.4546 mL
	80 mM	0.0436 mL	0.2182 mL	0.4364 mL	1.0909 mL
	100 mM	0.0349 mL	0.1746 mL	0.3491 mL	0.8728 mL

Ferruginol Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ferruginol514-62-5(+)-FerruginolEBVHSVApoptosisEpstein-Barr VirusHerpes simplex virusEBV-EAmitochondrial apoptosisantitumor and cardioprotective activitiesditerpenoidROSPI3K/AKTInhibitorinhibitorinhibit

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Ferruginol (Synonyms: (+)-Ferruginol)

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