1. Protein Tyrosine Kinase/RTK
    Anti-infection
  2. Src
    HIV
  3. Hck-IN-1

Hck-IN-1 

Cat. No.: HY-125028 Purity: 98.53%
Handling Instructions

Hck-IN-1 (compound B9), a diphenylpyrazolo compound, is a selective Nef-dependent Hck inhibitor with IC50s of 2.8 μM, >20 μM for Nef:Hck complex and Hck, respectively. Hck-IN-1 is a direct and wide HIV-1 Nef antagonists with an IC50 of 100-300 nM for wild-type HIV-1 replication. Hck-IN-1 binds pocket residue Asn126 and has anti-retroviral activity.

For research use only. We do not sell to patients.

Hck-IN-1 Chemical Structure

Hck-IN-1 Chemical Structure

CAS No. : 1473404-51-1

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 385 In-stock
Estimated Time of Arrival: December 31
5 mg USD 350 In-stock
Estimated Time of Arrival: December 31
10 mg USD 590 In-stock
Estimated Time of Arrival: December 31
50 mg USD 1800 In-stock
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100 mg USD 2800 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

Hck-IN-1 (compound B9), a diphenylpyrazolo compound, is a selective Nef-dependent Hck inhibitor with IC50s of 2.8 μM, >20 μM for Nef:Hck complex and Hck, respectively. Hck-IN-1 is a direct and wide HIV-1 Nef antagonists with an IC50 of 100-300 nM for wild-type HIV-1 replication. Hck-IN-1 binds pocket residue Asn126 and has anti-retroviral activity[1].

IC50 & Target[1]

HIV-1 Nef

100-300 nM (IC50)

In Vitro

Hck-IN-1 (compound B9) shows weak activity against other Src-family members in vitro, with IC50 values >20 μM for c-Src, Lck and Lyn[1].
B9 (1 μM; 8 days) completely inhibits Nef-dependent SFK activation at a concentration of 1.0 μM[1].
Hck-IN-1 (0.1, 0.3, 1, 3 μM) also inhibits Nef-mediated enhancement of HIV-1 infectivity in a concentration-dependent manner in the reporter cell line, TZM-bl[1].
Hck-IN-1 inhibits the replication of all eleven HIV-1 Nef chimeras with IC50 values of ~ 300 nM in CEM-T4 cells, demonstrating that the compound is broadly active against HIV replication supported by a wide range of HIV-1 Nef proteins[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: CEM-T4 cells
Concentration: 1 μM
Incubation Time: 8 days
Result: Completely inhibited Nef-dependent SFK activation at a concentration of 1.0 μM.
Molecular Weight

402.81

Formula

C₁₆H₁₁ClN₆O₃S

CAS No.

1473404-51-1

SMILES

S=C(N1N=C(C2=CC=C([N+]([O-])=O)C=C2)C(/N=N/C3=CC=CC(Cl)=C3)=C1O)N

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 12.5 mg/mL (31.03 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4826 mL 12.4128 mL 24.8256 mL
5 mM 0.4965 mL 2.4826 mL 4.9651 mL
10 mM 0.2483 mL 1.2413 mL 2.4826 mL
*Please refer to the solubility information to select the appropriate solvent.
References

Purity: 98.53%

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Keywords:

Hck-IN-1SrcHIVHuman immunodeficiency virusdiphenylpyrazoloNef-dependentHckNef:HckNefreplicationAsn126anti-retroviralInhibitorinhibitorinhibit

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Hck-IN-1
Cat. No.:
HY-125028
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