MMP-2/MMP-9 Substrate
Based on 1 Customer Validation
MMP-2/MMP-9 Substrate (Ac-Pro-Leu-Gly-[(S)-2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OEt) is a synthetic chromogenic polypeptide substrate whose core structure mimics the cleavage sites of MMP-2 and MMP-9 (gelatinase A and B) in collagen. After being hydrolyzed by collagenase, MMP-2/MMP-9 Substrate reacts with 4,4'-dithiodipyridine or Ellman's Reagent via its thiol fragment to produce a product with ultraviolet absorption properties.
For research use only. We do not sell to patients.
- Purity: 98.48%
- CAS No.: 99828-55-4
- Formula: C31H53N5O8S
- Molecular Weight:655.85
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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MMP-2 |
MMP-9 |
MMP-2/MMP-9 Substrate can be hydrolyzed by human skin fibroblast collagenase, human skin gelatinase, elastase, kallikrein, α-chymotrypsin, urokinase, trypsin, carboxypeptidase b, and bacterial collagenase[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Ac-Pro-Leu-{Gly-[(S)-2-mercapto-4-methyl-pentanoyl]}-Leu-{Gly-OEt}
Ac-PL-{Gly-[(S)-2-mercapto-4-methyl-pentanoyl]}-L-{Gly-OEt}
Chemical Information
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CAS No. 99828-55-4
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Appearance Solid
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Molecular Weight 655.85
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Formula C31H53N5O8S
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Color White to off-white
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Synonyms
Ac-Pro-Leu-Gly-[(S)-2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OEt
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (152.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Purity & Documentation
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Data Sheet (272 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
[1]. Weingarten H, et al. Spectrophotometric assay for vertebrate collagenase. Anal Biochem. 1985;147(2):437-440. [Content Brief]
[2]. Lupia E, et al. Pentosan polysulfate inhibits atherosclerosis in Watanabe heritable hyperlipidemic rabbits: differential modulation of metalloproteinase-2 and -9. Lab Invest. 2012;92(2):236-245. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5247 mL | 7.6237 mL | 15.2474 mL | 38.1185 mL |
| 5 mM | 0.3049 mL | 1.5247 mL | 3.0495 mL | 7.6237 mL | |
| 10 mM | 0.1525 mL | 0.7624 mL | 1.5247 mL | 3.8118 mL | |
| 15 mM | 0.1016 mL | 0.5082 mL | 1.0165 mL | 2.5412 mL | |
| 20 mM | 0.0762 mL | 0.3812 mL | 0.7624 mL | 1.9059 mL | |
| 25 mM | 0.0610 mL | 0.3049 mL | 0.6099 mL | 1.5247 mL | |
| 30 mM | 0.0508 mL | 0.2541 mL | 0.5082 mL | 1.2706 mL | |
| 40 mM | 0.0381 mL | 0.1906 mL | 0.3812 mL | 0.9530 mL | |
| 50 mM | 0.0305 mL | 0.1525 mL | 0.3049 mL | 0.7624 mL | |
| 60 mM | 0.0254 mL | 0.1271 mL | 0.2541 mL | 0.6353 mL | |
| 80 mM | 0.0191 mL | 0.0953 mL | 0.1906 mL | 0.4765 mL | |
| 100 mM | 0.0152 mL | 0.0762 mL | 0.1525 mL | 0.3812 mL |
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