Cat. No.: HY-N0770

Isoliensinine

4 Cited Publications

Customer Review

Based on 4 publication(s) in Google Scholar

Isoliensinine is a bisbenzylisoquinoline alkaloid extracted from the seed embryo of Nelumbo nucifera, with anti-oxidant and anti-inflammatory and anti-cancer activities. Isoliensinine induces apoptosis in triple-negative human breast cancer cells.

For research use only. We do not sell to patients.

Purity: 99.90%
CAS No.: 6817-41-0
Formula: C₃₇H₄₂N₂O₆
Molecular Weight:610.74

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Isoliensinine Chemical Structure

CAS No.: 6817-41-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Publications (4)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
3T3-L1	IC₅₀	7.8 μM Compound: 10	Anti-adipogenic activity in mouse 3T3-L1 cells transfected with Lenti-PPARgamma-Luc reporter assessed as inhibition of PPARgamma expression by luminescence based microplate reader analysis Anti-adipogenic activity in mouse 3T3-L1 cells transfected with Lenti-PPARgamma-Luc reporter assessed as inhibition of PPARgamma expression by luminescence based microplate reader analysis	[PMID: 38483204]

In Vitro

Isoliensinine induces apoptosis in triple-negative human breast cancer cells through ROS generation and p38 MAPK/JNK activation^[1].
Isoliensinine exerts antiproliferative effect on CASMCs induced by phenylephrine, and its mechanisms are related to decrease the overexpression of growth factors (PDGF-beta, bFGF), protooncogene (c-fos, c-myc) and hsp70^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 6817-41-0
Appearance Solid
Molecular Weight 610.74
Formula C₃₇H₄₂N₂O₆
Color White to off-white
SMILES

OC1=CC2=C(C=C1OC)CCN(C)[C@@H]2CC3=CC=C(O)C(OC4=CC5=C(C=C4OC)CCN(C)[C@@H]5CC6=CC=C(OC)C=C6)=C3
Structure Classification
- Alkaloids
- Isoquinoline Alkaloids
- Phenols
- Monophenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (4)

All Cell Proliferation/Viability AssayApoptosis AnalysisWBCell Imaging/Staining

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Journal Impact Factor

Journal of Future Foods

2025 Nov 6.
Pharmaceuticals (Basel)

Isoliensinine Induces Ferroptosis in Urothelial Carcinoma Cells via the PI3K/AKT/HIF-1α Axis: Molecular Evidence from Next-Generation Sequencing. [Abstract]

2025 Jul 6;18(7):1008. PMID: 40732297

Isoliensinine purchased from MedChemExpress. Usage Cited in: Pharmaceuticals (Basel). 2025 Jul 6;18(7):1008. [Abstract]

An MTT assay was performed to measure the viability of T24 and UMUC3 cells after treatment with Isoliensinine (0, 20, 40, and 80 μM) for 48 h.

Isoliensinine purchased from MedChemExpress. Usage Cited in: Pharmaceuticals (Basel). 2025 Jul 6;18(7):1008. [Abstract]

Flow cytometry using annexin V/propidium iodide dual staining showed that apoptosis was induced in UC cells by Isoliensinine treatment at 0, 20, and 80 μM for 48 h.

Isoliensinine purchased from MedChemExpress. Usage Cited in: Pharmaceuticals (Basel). 2025 Jul 6;18(7):1008. [Abstract]

Western blots of apoptosis-related proteins in T24 after treatment with isoliensinine for 2 days and in UMUC3 after treatment with Isoliensinine (20, 80 μM) for 4 days.

Isoliensinine purchased from MedChemExpress. Usage Cited in: Pharmaceuticals (Basel). 2025 Jul 6;18(7):1008. [Abstract]

A colony formation assay was performed on T24 and UMUC3 cells after Isoliensinine treatment (0, 20, and 40 μM) for 2 days, followed by incubation in untreated medium for 7 days.
Molecules

Molecular Mechanisms Underlying the Anti-Tumor Activity of Lotus-Derived Alkaloids in Breast Cancer. [Abstract]

2026 Mar 12;31(6):947. PMID: 41900047
bioRxiv

2025 July 11.

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (81.87 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.6374 mL

8.1868 mL

16.3736 mL

5 mM

0.3275 mL

1.6374 mL

3.2747 mL

10 mM

0.1637 mL

0.8187 mL

1.6374 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

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Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (4.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (4.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Jun-hua Xiao, et al. Effects of isoliensinine on proliferation of porcine coronary arterial smooth muscle cells induced by phenylephrine. Yao Xue Xue Bao. 2005 Feb;40(2):105-10. [Content Brief]

[2]. Zhang X, et al. Isoliensinine induces apoptosis in triple-negative human breast cancer cells through ROS generation and p38 MAPK/JNK activation. Sci Rep. 2015 Jul 29;5:12579. [Content Brief]

[3]. Xiao JH, et al. Effects of isoliensinine on proliferation of porcine coronary arterial smooth muscle cells induced by phenylephrine. Yao Xue Xue Bao. 2005 Feb;40(2):105-10.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.6374 mL	8.1868 mL	16.3736 mL	40.9339 mL
	5 mM	0.3275 mL	1.6374 mL	3.2747 mL	8.1868 mL
	10 mM	0.1637 mL	0.8187 mL	1.6374 mL	4.0934 mL
	15 mM	0.1092 mL	0.5458 mL	1.0916 mL	2.7289 mL
	20 mM	0.0819 mL	0.4093 mL	0.8187 mL	2.0467 mL
	25 mM	0.0655 mL	0.3275 mL	0.6549 mL	1.6374 mL
	30 mM	0.0546 mL	0.2729 mL	0.5458 mL	1.3645 mL
	40 mM	0.0409 mL	0.2047 mL	0.4093 mL	1.0233 mL
	50 mM	0.0327 mL	0.1637 mL	0.3275 mL	0.8187 mL
	60 mM	0.0273 mL	0.1364 mL	0.2729 mL	0.6822 mL
	80 mM	0.0205 mL	0.1023 mL	0.2047 mL	0.5117 mL

Keywords

Isoliensinine

4 Cited Publications

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Isoliensinine

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Natural Product Library

Anti-Cancer Compound Library

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Publications Citing Use of MedChemExpress (MCE) Isoliensinine

Biological Activity

Chemical Information

Publications (4)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Isoliensinine Related Classifications

Cat. No.: HY-N0770