BF6
BF6 is a blood-brain barrier permeable NIR probe. BF6 can diffuse from intracranial blood vessels into brain tissue, enabling visualization of brain structures and differentiation between glioblastoma (GBM) and normal tissues. The absorption peaks of BF6 in DMSO and FBS range from 727 to 859 nm, with a broad fluorescence emission band spanning 800 to 1400 nm; its emission wavelength falls within the NIR-II region (800-1400 nm).
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 分子式: C20H22BF2N7O
- 分子量:425.24
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
Operating Instructions (The following is a recommended experimental protocol for guidance only; please adjust it according to your specific requirements).
1. Staining Procedure[1]
1.1 Sample Type: Adherent cells (U-87 MG cells)
1.1.1 Adherent cells: Trypsin digestion is not required for the staining procedure.
1.2 Incubation Conditions: Incubate with 2 μM BF6 for 20 min; use 5 μM BF6 for incubation in an in vitro blood-brain barrier (BBB) model.
1.3 Washing Step: Wash the cells after incubation to remove excess dye.
2. Control Setup
2.1 Evans Blue serves as the negative control for in vitro BBB permeability assays.
3. Detection and Analysis
3.1 Instrument: NIR-II microscope.
3.1.1 Cell Imaging Parameters: Ex = 663 nm, Em = 950 nm.
3.2 Result Analysis:
3.2.1 Fluorescence Intensity Change: Bright NIR-II fluorescence is exhibited in the cellular environment.
3.2.2 Fluorescence Localization: Targets intracellular lipid droplets.
3.2.3 Color Change: Presents pseudo-colored NIR-II fluorescence with a gradient from low to high intensity.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
BF6 (200 μM; tail vein injection) distinguishes normal brain tissue from glioblastoma tissue in mouse models, exhibits higher fluorescence intensity in normal tissues, and enables clear delineation of tumor boundaries via NIR-II imaging[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Athymic nude mice[1]
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Dosage:200 μM
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Administration:i.v. via tail vein
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Result:Detected significant NIR-II fluorescence signals in brain tissue after injection.
Reached maximum fluorescence intensity at 45 min post-injection, with a signal-to-noise ratio range of 2.5-2.9.
Showed significant accumulation in brain tissue via ex vivo imaging at 2 h post-injection.
Detected a peak at m/z + H = 426.2 (corresponding to BF6) in brain homogenates via mass spectrometry.
Confirmed accumulation in brain tissue via HPLC analysis.
Increased the NIR-II fluorescence ratio between brain tissue and vessels within 160 s, with tissue intensity overtaking vessel intensity at 100 s post-injection.
Exhibited negligible toxicity, with H&E staining of major organs collected 1 month post-injection showing negligible toxicity compared to PBS-treated mice.
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Animal Model:Athymic nude mice (intracranial implantation of U-87 MG GBM cells, allowed to grow for 3-4 weeks)[1]
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Dosage:200 μM
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Administration:i.v. via tail vein
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Result:
Showed uneven brain fluorescence imaging after injection, with fluorescence intensity of partial brain tissues increasing from 0 to 60 min.
Mirrored in vivo results via ex vivo imaging.
Exhibited higher fluorescence intensity in normal brain tissue than glioma tissue, allowing clear differentiation and delineation of tumor margins.
Achieved a normal-to-glioma fluorescence ratio significantly larger than that observed with ICG, indicating superior differentiation capability.
化学情報
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分子量 425.24
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分子式 C20H22BF2N7O
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SMILES
CN(C1=CC=C(C=C1)[N+]2=NC(C#N)=NN([B-]2(F)F)C3=CC=C(C=C3)N4CCOCC4)C
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
BF6 関連分類
HY-D3113 関連分類
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