MMP-2/MMP-9 Substrate
Based on 1 Customer Validation
MMP-2/MMP-9 Substrate (Ac-Pro-Leu-Gly-[(S)-2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OEt) is a synthetic chromogenic polypeptide substrate whose core structure mimics the cleavage sites of MMP-2 and MMP-9 (gelatinase A and B) in collagen. After being hydrolyzed by collagenase, MMP-2/MMP-9 Substrate reacts with 4,4'-dithiodipyridine or Ellman's Reagent via its thiol fragment to produce a product with ultraviolet absorption properties.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 98.48%
- CAS 番号: 99828-55-4
- 分子式: C31H53N5O8S
- 分子量:655.85
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保管条件:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
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MMP-2 |
MMP-9 |
MMP-2/MMP-9 Substrate can be hydrolyzed by human skin fibroblast collagenase, human skin gelatinase, elastase, kallikrein, α-chymotrypsin, urokinase, trypsin, carboxypeptidase b, and bacterial collagenase[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 99828-55-4
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性状 Solid
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分子量 655.85
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分子式 C31H53N5O8S
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Color White to off-white
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別名
Ac-Pro-Leu-Gly-[(S)-2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OEt
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配列
Ac-Pro-Leu-{Gly-[(S)-2-mercapto-4-methyl-pentanoyl]}-Leu-{Gly-OEt}
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シーケンスの短縮
Ac-PL-{Gly-[(S)-2-mercapto-4-methyl-pentanoyl]}-L-{Gly-OEt}
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
DMSO : 100 mg/mL (152.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (272 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Weingarten H, et al. Spectrophotometric assay for vertebrate collagenase. Anal Biochem. 1985;147(2):437-440. [Content Brief]
[2]. Lupia E, et al. Pentosan polysulfate inhibits atherosclerosis in Watanabe heritable hyperlipidemic rabbits: differential modulation of metalloproteinase-2 and -9. Lab Invest. 2012;92(2):236-245. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5247 mL | 7.6237 mL | 15.2474 mL | 38.1185 mL |
| 5 mM | 0.3049 mL | 1.5247 mL | 3.0495 mL | 7.6237 mL | |
| 10 mM | 0.1525 mL | 0.7624 mL | 1.5247 mL | 3.8118 mL | |
| 15 mM | 0.1016 mL | 0.5082 mL | 1.0165 mL | 2.5412 mL | |
| 20 mM | 0.0762 mL | 0.3812 mL | 0.7624 mL | 1.9059 mL | |
| 25 mM | 0.0610 mL | 0.3049 mL | 0.6099 mL | 1.5247 mL | |
| 30 mM | 0.0508 mL | 0.2541 mL | 0.5082 mL | 1.2706 mL | |
| 40 mM | 0.0381 mL | 0.1906 mL | 0.3812 mL | 0.9530 mL | |
| 50 mM | 0.0305 mL | 0.1525 mL | 0.3049 mL | 0.7624 mL | |
| 60 mM | 0.0254 mL | 0.1271 mL | 0.2541 mL | 0.6353 mL | |
| 80 mM | 0.0191 mL | 0.0953 mL | 0.1906 mL | 0.4765 mL | |
| 100 mM | 0.0152 mL | 0.0762 mL | 0.1525 mL | 0.3812 mL |
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