1. Saccharides and Glycosides
  2. Laminaran

Laminaran 

Cat. No.: HY-119109
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Laminaran is a neutral water-soluble β-1-3-glucan and a typical ligand for Dectin-1 isolated from the brown alga D. dichotoma, has potent immunomodulating, radioprotective, and anticancer activities. Laminaran is made up of β (1→3)-glucan with β (1→6)-branches and can be catalyzed by enzymes such as laminarinase (EC 3.2.1.6) that breaks the β (1→3) bonds. Laminaran is a promising immune stimulatory molecule for use in cancer immunotherapy.

For research use only. We do not sell to patients.

Laminaran Chemical Structure

Laminaran Chemical Structure

CAS No. : 9008-22-4

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Estimated Time of Arrival: December 31
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Description

Laminaran is a neutral water-soluble β-1-3-glucan and a typical ligand for Dectin-1 isolated from the brown alga D. dichotoma, has potent immunomodulating, radioprotective, and anticancer activities[1]. Laminaran is made up of β (1→3)-glucan with β (1→6)-branches and can be catalyzed by enzymes such as laminarinase (EC 3.2.1.6) that breaks the β (1→3) bonds[2]. Laminaran is a promising immune stimulatory molecule for use in cancer immunotherapy[3].

In Vitro

Laminaran (100-800 µg/mL; 24 hours) is not cytotoxic against normal epidermal cells JB6 Cl41 and human melanoma cells SK-MEL-28, the percentage of inhibition of living cells number is less than 15 % at concentrations range up to 800 µg/mL after 24 h of treatment[1].
Laminaran (200 µg/mL; 24-72 hours)does not cause any growth inhibition of SK-MEL-28 cells after 24 and 48 h of treatment, but decreases cells proliferation by 36 % after 72 h of treatment[1].
Laminaran (25-50 µg/mL; 24 hours) at low concentration does not influence the phosphorylation of c-Raf (Ser259), ERK1/2 (Tyr202/Tyr204), and MEK1/2 (Ser 221) kinases as well as total expression level of investigated proteins. But decreases p-MEK, p-ERK1/2 at 50 µg/mL[1].

Cell Viability Assay[1]

Cell Line: JB6 Cl41 and SK-MEL-28 cells
Concentration: 100, 200, 400, and 800 µg/mL
Incubation Time: 24 hours
Result: Showed no cytotoxic against normal epidermal cells JB6 Cl41 and human melanoma cells SK-MEL-28.

Cell Proliferation Assay[1]

Cell Line: SK-MEL-28 cells
Concentration: 200 µg/mL
Incubation Time: 24 hours
Result: Decreased cell proliferation at 72 hours.

Western Blot Analysis[1]

Cell Line: SK-MEL-28 cells
Concentration: 25 µg/mL; 50 µg/mL
Incubation Time: 24 hours
Result: Inhibited phosphorylation of c-Raf, MEK1/2, and ERK1/2 kinases.
In Vivo

Laminaran (intravenous injection; 12.5, 25, and 50 mg/kg; 21 days) and OVA (50 μg) combination significanly decreases the tumor masses when it compares with the PBS-, OVA-, and laminarin-treated mice[3].

Animal Model: C57BL/6 mice injected s.c. with B16-OVA cells[3]
Dosage: 12.5, 25, and 50 mg/kg; 21 days
Administration: Intravenous injection
Result: Prevented B16-OVA tumor growth by inducing Ag-specific immune responses.
CAS No.

9008-22-4

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Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (Need ultrasonic)

In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (Infinity mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (Infinity mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

LaminaranOthersImmunomodulatingradioprotectiveanticancer immunotherapytumorInhibitorinhibitorinhibit

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Laminaran
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