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  5. Laminaran

Laminaran is an β-1-3-glucan and a typical ligand for Dectin-1 from Eisenia Bicyclis, has potent immunomodulating, radioprotective, and anticancer activities. Laminaran is made up of β (1→3)-glucan with β (1→6)-branches and can be catalyzed by enzymes such as laminarinase (EC 3.2.1.6) that breaks the β (1→3) bonds. Laminaran is a promising immune stimulatory molecule for use in cancer immunotherapy.

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Laminaran Chemical Structure

Laminaran Chemical Structure

CAS No. : 9008-22-4

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Laminaran Related Antibodies

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Description

Laminaran is an β-1-3-glucan and a typical ligand for Dectin-1 from Eisenia Bicyclis, has potent immunomodulating, radioprotective, and anticancer activities[1]. Laminaran is made up of β (1→3)-glucan with β (1→6)-branches and can be catalyzed by enzymes such as laminarinase (EC 3.2.1.6) that breaks the β (1→3) bonds[2]. Laminaran is a promising immune stimulatory molecule for use in cancer immunotherapy[3].

In Vitro

Laminaran (100-800 µg/mL; 24 hours) is not cytotoxic against normal epidermal cells JB6 Cl41 and human melanoma cells SK-MEL-28, the percentage of inhibition of living cells number is less than 15 % at concentrations range up to 800 µg/mL after 24 h of treatment[1].
Laminaran (200 µg/mL; 24-72 hours)does not cause any growth inhibition of SK-MEL-28 cells after 24 and 48 h of treatment, but decreases cells proliferation by 36 % after 72 h of treatment[1].
Laminaran (25-50 µg/mL; 24 hours) at low concentration does not influence the phosphorylation of c-Raf (Ser259), ERK1/2 (Tyr202/Tyr204), and MEK1/2 (Ser 221) kinases as well as total expression level of investigated proteins. But decreases p-MEK, p-ERK1/2 at 50 µg/mL[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Laminaran Related Antibodies

Cell Viability Assay[1]

Cell Line: JB6 Cl41 and SK-MEL-28 cells
Concentration: 100, 200, 400, and 800 µg/mL
Incubation Time: 24 hours
Result: Showed no cytotoxic against normal epidermal cells JB6 Cl41 and human melanoma cells SK-MEL-28.

Cell Proliferation Assay[1]

Cell Line: SK-MEL-28 cells
Concentration: 200 µg/mL
Incubation Time: 24 hours
Result: Decreased cell proliferation at 72 hours.

Western Blot Analysis[1]

Cell Line: SK-MEL-28 cells
Concentration: 25 µg/mL; 50 µg/mL
Incubation Time: 24 hours
Result: Inhibited phosphorylation of c-Raf, MEK1/2, and ERK1/2 kinases.
In Vivo

Laminaran (intravenous injection; 12.5, 25, and 50 mg/kg; 21 days) and OVA (50 μg) combination significanly decreases the tumor masses when it compares with the PBS-, OVA-, and laminarin-treated mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 mice injected s.c. with B16-OVA cells[3]
Dosage: 12.5, 25, and 50 mg/kg; 21 days
Administration: Intravenous injection
Result: Prevented B16-OVA tumor growth by inducing Ag-specific immune responses.
Formula

C18H32O16
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC[C@H]1OC(O[C@H]2[C@H](O)[C@@H](CO)OC(O[C@@H]3[C@@H](O)C(O[H])O[C@H](CO)[C@H]3O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O.[n]
Structure Classification
Initial Source

Eisenia Bicyclis
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 16.67 mg/mL (Need ultrasonic)

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In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 33.33 mg/mL (Infinity mM); Clear solution; Need ultrasonic

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Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

COA (250 KB)

Handling Instructions (2659 KB)
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Laminaran9008-22-4OthersImmunomodulatingradioprotectiveanticancer immunotherapytumorInhibitorinhibitorinhibit

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