MCH(human, mouse, rat)  (Synonyms: Melanin-concentrating hormone(human, mouse, rat))

MCH (human, mouse, rat) is a cyclic neuropeptide mainly synthesized by neurons in the lateral hypothalamic area. MCH (human, mouse, rat) also serves as an endogenous ligand for the melanin-concentrating hormone receptor (MHC receptor), with a binding IC₅₀ of 0.3 nM and 1.5 nM for human MCH-1R and MCH-2R, respectively; its functional EC₅₀ values are 3.9 nM and 88.7 nM. MCH (human, mouse, rat) acts not only as an orexigenic signal but also as a key integrating and regulatory hormone for energy homeostasis and sleep-wake cycles. MCH (human, mouse, rat) can be used in studies related to obesity, sleep disorders, and other associated conditions^[1][2][3][4].

Binding IC50: 0.3 nM (MCH1R)
IC50: 1.5 nM (MCH1R)^[1]

MCH (human, mouse, rat) potently displaces the binding of [¹²⁵I]Phe¹³-Tyr¹⁹-MCH to MCH-2R on the membranes of transfected COS-7 and CHO cells, with an IC₅₀ of 1.5 nM^[2].
MCH (human, mouse, rat) (60 min) dose-dependently activates calcium mobilization in CHO cells stably expressing human MCH-2R, with an EC₅₀ of 0.1 nM^[2].
MCH (human, mouse, rat) (30 s) induces a robust, dose-dependent intracellular calcium response in HEK293-AEQ17 cells stably expressing human MCH-2R, with an EC₅₀ of 31 nM^[2].
MCH (human, mouse, rat) (1 h) potently stimulates inositol phosphate turnover in HEK293-AEQ17 cells stably expressing human MCH-2R, with an EC₅₀ of 2.7 nM^[2].
MCH (human, mouse, rat) reduces the release of α-MSH and secretion of CART in rodent hypothalamic tissue explants, and induces the release of NPY and AgRP^[3].
MCH (human, mouse, rat) induces dose-dependent GIRK currents in Xenopus laevis oocytes co-expressing SLC-1 receptors and GIRK channels^[4].
MCH (human, mouse, rat) induces calcium-mediated chloride currents in Xenopus laevis oocytes expressing the SLC-1 receptor by activating the phospholipase C pathway^[4].
MCH (human, mouse, rat) exerts inhibitory effects on primary cultured rat LH neurons, including reducing spontaneous firing frequency, suppressing glutamatergic synaptic transmission, and decreasing the amplitude of VDCC via a Gi/o-dependent pathway, with an IC₅₀ of 7.84 nM, and it does not induce GIRK currents^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Administration of MCH (human, mouse, rat) (into the medial preoptic area) reduces the active maternal behavioral components in lactating rats^[1].
Levels of MCH (human, mouse, rat) are upregulated in fasted rats, leptin-deficient ob/ob obese mice, fa/fa rats, and diet-induced obese rats^[2].
form of MCH (human, mouse, rat) (administered intracerebroventricularly; acute single injection; chronic infusion) exerts orexigenic effects in Wistar rats and Sprague-Dawley rats, including increased acute food intake and obesity induced by chronic infusion^[3].
form of MCH (human, mouse, rat) (intracerebral/intracerebroventricular; chronic administration) increases food intake, body weight and obesity in mice, while overexpression of this substance in the lateral hypothalamus induces obesity and insulin resistance^[4].
form of MCH (human, mouse, rat) (administered intracerebrally; administered intracerebroventricularly; administered locally in the hypothalamus; acutely administered) stimulates food intake in rats^[4].
MCH (human, mouse, rat) regulates memory retention in rats^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2386.84

C₁₀₅H₁₆₀N₃₀O₂₆S₄

128315-56-0

Solid

DFDMLRCMLGRVYRPCWQV (Disulfide bridge:Cys7-Cys16)

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Diniz GB, et al. The Melanin-Concentrating Hormone (MCH) System: A Tale of Two Peptides. Front Neurosci. 2019;13:1280. Published 2019 Nov 26.

  [2]. A W Sailer, et al. Identification and characterization of a second melanin-concentrating hormone receptor, MCH-2R. Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7564-9.  [Content Brief]

  [3]. Griffond B, et al. MCH and feeding behavior-interaction with peptidic network. Peptides. 2009;30(11):2045-2051.

  [4]. Gao XB, et al. Electrophysiological effects of MCH on neurons in the hypothalamus. Peptides. 2009;30(11):2025-2030.