2. Metabolic Enzyme/Protease
  3. Mitochondrial Metabolism
  4. MCU-i4

MCU-i4 blocks the IP3-dependent mitochondrial Ca2+-uptake, maintaining the gatekeeping role of their target.

For research use only. We do not sell to patients.

CAS No. : 371924-24-2

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10 mM * 1 mL in DMSO
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Customer Review

Based on 5 publication(s) in Google Scholar

MCU-i4 Related Antibodies

Top Publications Citing Use of Products

    MCU-i4 purchased from MedChemExpress. Usage Cited in: Brain Sci. 2025 Oct 28;15(11):1157.  [Abstract]

    MCU-i4 (0-20 μM). Western blot results of MCU protein expression.

    MCU-i4 purchased from MedChemExpress. Usage Cited in: Brain Sci. 2025 Oct 28;15(11):1157.  [Abstract]

    Inhibiting MCU expression using MCU-i4 attenuated the high glucose-induced increase in p-IRE1α fluorescence intensity, while inhibiting IRE1α phosphorylation using 4μ8C reduced the glucose-induced increase in MCU fluorescence intensity. Immunofluorescence images of MCU and p-IRE1α proteins in hippocampal neurons.

    MCU-i4 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2022 Aug 3;23(15):8629.

    The expression of MICU1 protein in vitrified oocytes treated with or without MCU-i4 (2 μM) was analyzed.

    MCU-i4 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2022 Aug 3;23(15):8629.

    Effects of MCU-i4 or DS16570511 treatment on oocyte quality during vitrification and freezing; fluorescence images of MMPs in each group.

    MCU-i4 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2022 Aug 3;23(15):8629.

    Effects of MCU-i4 or DS16570511 treatment on pyruvate oxidation and energy metabolism in vitrified oocytes. Western blot analysis of p-PDH, PDH, and their expression ratios in oocytes of each group.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    MCU-i4 blocks the IP3-dependent mitochondrial Ca2+-uptake, maintaining the gatekeeping role of their target[1][2].
    In Vitro

    MCU-i4 modulates mitochondrial Ca2+ uptake to 1 min of incubation, i.e., exerting a negative modulation, indicating that the primary effect of these compounds is a sudden and direct modulation of MCU complex activity[1].
    MCU-i4 exerted a negative effect on Δψ. MCU-i4 has no effect on cell viability[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    MCU-i4 Related Antibodies

    Cell Differentiation Assay[1]

    Cell Line: Growing medium of 90% confluent C2C12 myoblasts.
    Concentration: 10 μM.
    Incubation Time: 24 h.
    Result: Decreased myotube width.
    Molecular Weight

    377.48

    Formula

    C23H27N3O2
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C(C1=C(NC2=CC=C(N(CC)CC)C=C2)C3=CC(C)=CC=C3N=C1)OCC
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 12.5 mg/mL (33.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.6491 mL 13.2457 mL 26.4915 mL
    5 mM 0.5298 mL 2.6491 mL 5.2983 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

    =
    Concentration (final)

    C2

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    Volume (final)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 1.25 mg/mL (3.31 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 1.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 1.25 mg/mL (3.31 mM); Clear solution

      This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  50% PEG300    50% Saline

      Solubility: 2 mg/mL (5.30 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.87%

    SDS (392 KB)

    COA (251 KB) LCMS (94 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.6491 mL 13.2457 mL 26.4915 mL 66.2287 mL
    5 mM 0.5298 mL 2.6491 mL 5.2983 mL 13.2457 mL
    10 mM 0.2649 mL 1.3246 mL 2.6491 mL 6.6229 mL
    15 mM 0.1766 mL 0.8830 mL 1.7661 mL 4.4152 mL
    20 mM 0.1325 mL 0.6623 mL 1.3246 mL 3.3114 mL
    25 mM 0.1060 mL 0.5298 mL 1.0597 mL 2.6491 mL
    30 mM 0.0883 mL 0.4415 mL 0.8830 mL 2.2076 mL
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    MCU-i4 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    MCU-i4371924-24-2Mitochondrial MetabolismmitochondrialCa2+uptakeC2C12myoblastInhibitorinhibitorinhibit

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