A selective turn-on fluorescent sensor for imaging copper in living cells

  • J Am Chem Soc. 2006 Jan 11;128(1):10-1. doi: 10.1021/ja055064u.
Li Zeng  1 ,  Evan W Miller ,  Arnd Pralle ,  Ehud Y Isacoff ,  Christopher J Chang
Affiliations
  • 1. Department of Chemistry, University of California, Berkeley, CA 94720, USA.
PMID: 16390096 DOI: 10.1021/ja055064u
Abstract

We present the synthesis, properties, and biological applications of Coppersensor-1 (CS1), a new water-soluble, turn-on fluorescent sensor for intracellular imaging of copper in living biological samples. CS1 utilizes a BODIPY reporter and thioether-rich receptor to provide high selectivity and sensitivity for Cu+ over Other biologically relevant metal ions, including Cu2+, in aqueous solution. This BODIPY-based probe is the first Cu+-responsive sensor with visible excitation and emission profiles and gives a 10-fold turn-on response for detecting this ion. Confocal microscopy experiments further establish that CS1 is membrane-permeable and can successfully monitor intracellular Cu+ levels within living cells.

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