Coppersensor 1 (solution)

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)^[1][2].
1. Preparation of reagent stock solutions for imaging.
2. Viable cell labeling:
2.1 Imaging experiments with cells will require 1-2 d with cells in culture. Incubate the cells according to your normal protocol.
2.2 One day before imaging, cells are passaged and plated on glass coverslips coated with poly-L-lysine (50 μg/mL). Adherent cells for imaging are grown to 50-80% confluency.
2.3 Remove cells from incubator, and transfer one coverslip into a Petri dish containing PBS buffer.
2.4 To the Petri dish, add Coppersensor 1 to give a final dye concentration of 5 μM. Mix thoroughly (Higher concentrations of dye may result in high levels of background fluorescence).
2.5 Incubate for 5-20 min in the dark at 25 or 37°C.
3. Imaging the labeled cells:
3.1 Imageing in standard confocal microscope with 543 nm excitation (Coppersensor 1 can be imaged using any type of fluorescence microscope, including epifluorescence, confocal and multiphoton).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.