Coppersensor 1 (solution) 

Coppersensor 1 (solution) is a membrane-permeable fluorescent dye. Coppersensor-1 has a picomolar affinity for Cu⁺ with high selectivity over competing cellular metalions. Coppersensor-1 as a probe, can selective and sensitive detection of copper(I) ions (Cu⁺) in biological samples, including live cells. Coppersensor-1 can be used for the research of imaging of severe diseases such as cancer, cardiovascular disorders and neurogenerative diseases^[1].
Solvent and concentration: DMSO: 2 mM

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)^[1][2].
1. Preparation of reagent stock solutions for imaging.
2. Viable cell labeling:
2.1 Imaging experiments with cells will require 1-2 d with cells in culture. Incubate the cells according to your normal protocol.
2.2 One day before imaging, cells are passaged and plated on glass coverslips coated with poly-L-lysine (50 μg/mL). Adherent cells for imaging are grown to 50-80% confluency.
2.3 Remove cells from incubator, and transfer one coverslip into a Petri dish containing PBS buffer.
2.4 To the Petri dish, add Coppersensor 1 to give a final dye concentration of 5 μM. Mix thoroughly (Higher concentrations of dye may result in high levels of background fluorescence).
2.5 Incubate for 5-20 min in the dark at 25 or 37°C.
3. Imaging the labeled cells:
3.1 Imageing in standard confocal microscope with 543 nm excitation (Coppersensor 1 can be imaged using any type of fluorescence microscope, including epifluorescence, confocal and multiphoton).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

629.81

C₃₀H₅₀BF₂N₃S₄

874748-20-6

576

543

[F-][B+3]1([N]2=C(C)C(CC)=C(C)C2=C(CN(CCSCCSCC)CCSCCSCC)C3=C(C)C(CC)=C(C)[N-]31)[F-]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Evan W Miller, et al. Preparation and use of Coppersensor-1, a synthetic fluorophore for live-cell copper imaging. Nat Protoc. 2006;1(2):824-7.  [Content Brief]

  [2]. Li Zeng, et al. A selective turn-on fluorescent sensor for imaging copper in living cells. J Am Chem Soc. 2006 Jan 11;128(1):10-1.  [Content Brief]