Changes in gene expression induced by histamine, fexofenadine and osthole: Expression of histamine H1 receptor, COX-2, NF-κB, CCR1, chemokine CCL5/RANTES and interleukin-1β in PBMC allergic and non-allergic patients

  • Immunobiology. 2017 Mar;222(3):571-581. doi: 10.1016/j.imbio.2016.11.004.
Natalia Karolina Kordulewska  1 ,  Elżbieta Kostyra  2 ,  Anna Cieślińska  3 ,  Michał Matysiewicz  4 ,  Ewa Fiedorowicz  5 ,  Edyta Sienkiewicz-Szłapka  6
Affiliations
  • 1. University of Warmia and Mazury in Olsztyn, Olsztyn, Warmińsko-Mazurskie, Poland. Electronic address: [email protected].
  • 2. University of Warmia and Mazury in Olsztyn, Olsztyn, Warmińsko-Mazurskie, Poland. Electronic address: [email protected].
  • 3. University of Warmia and Mazury in Olsztyn, Olsztyn, Warmińsko-Mazurskie, Poland. Electronic address: [email protected].
  • 4. University of Warmia and Mazury in Olsztyn, Olsztyn, Warmińsko-Mazurskie, Poland. Electronic address: [email protected].
  • 5. University of Warmia and Mazury in Olsztyn, Olsztyn, Warmińsko-Mazurskie, Poland. Electronic address: [email protected].
  • 6. University of Warmia and Mazury in Olsztyn, Olsztyn, Warmińsko-Mazurskie, Poland. Electronic address: [email protected].
PMID: 27843000 DOI: 10.1016/j.imbio.2016.11.004
Abstract

Introduction: Fexofenadine (FXF) is a third-generation antihistamine drug and osthole is assumed as a natural antihistamine alternative. This paper compares results of histamine, FXF and osthole impact on HRH-1, COX-2, NF-κB-p50, CCR1 mRNA expression. We also measured mRNA expression of IL-1β and CCL5/RANTES in incubated peripheral blood mononuclear cells (PBMC) to compared how histamine, FXF and osthole had influence on expression level and interacts on product secretion.

Objective: The purpose was to investigate expression pattern in asthma PBMC.

Methods: The cultures were treated 72h with FXF and osthole. We measured mRNA expression of histamine HRH-1, COX-2, NF-κB-p50, CCR1, IL-1β and CCL5/RANTES with Real-Time PCR (RT-PCR).

Results: The present study suggest that osthole may be a potential inhibitor of histamine H1 receptor activity. We also demonstrated that cells cultured with histamine increase COX-2 mRNA expression and osthole reduce it.

Conclusion: Allergy remains one of the most common chronic diseases in Europe and it is rapidly approaching epidemic proportions; with current predictions estimating that the number of allergy-afflicted will equal the healthy population by 2020. It is therefore paramount to find new pharmaceuticals which successfully combat allergic disease.

Keywords
Allergic disease; Antihistamine drugs; Cultures in vitro; Expression in PBMCs.
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