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  5. CD79 Protein, Human (HEK293, Flag-His)

CD79 Protein, Human (HEK293, Flag-His)

Cat. No.: HY-P75386
Handling Instructions

CD79A Protein is crucial for initiating the B-cell antigen receptor complex (BCR) signal transduction cascade upon antigen binding. It facilitates BCR complex internalization, trafficking to late endosomes, and antigen presentation. CD79A ensures BCR surface expression, pro- and pre-B-cell differentiation, and autophosphorylation of SYK by binding to BLNK. It interacts with FYN and LYN kinases, enhancing their activity. CD79A, forming a disulfide-linked heterodimer with CD79B, constitutes the BCR complex. It interacts with SYK and BLNK, orchestrating essential signaling events in B-cell development. CD79 Protein, Human (HEK293, Flag-His) is a recombinant protein dimer complex containing human-derived CD79 protein, expressed by HEK293, with C-His, C-Flag labeled tag. CD79 Protein, Human (HEK293, Flag-His), has molecular weight of ~37.5 kDa.

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Description

CD79A Protein is crucial for initiating the B-cell antigen receptor complex (BCR) signal transduction cascade upon antigen binding. It facilitates BCR complex internalization, trafficking to late endosomes, and antigen presentation. CD79A ensures BCR surface expression, pro- and pre-B-cell differentiation, and autophosphorylation of SYK by binding to BLNK. It interacts with FYN and LYN kinases, enhancing their activity. CD79A, forming a disulfide-linked heterodimer with CD79B, constitutes the BCR complex. It interacts with SYK and BLNK, orchestrating essential signaling events in B-cell development. CD79 Protein, Human (HEK293, Flag-His) is a recombinant protein dimer complex containing human-derived CD79 protein, expressed by HEK293, with C-His, C-Flag labeled tag. CD79 Protein, Human (HEK293, Flag-His), has molecular weight of ~37.5 kDa.

Background

The CD79A protein is essential for the initiation of the signal transduction cascade in response to antigen binding to the B-cell antigen receptor complex (BCR). It plays a crucial role in internalizing the BCR complex, trafficking it to late endosomes, and facilitating antigen presentation. CD79A is also necessary for the surface expression of the BCR and efficient differentiation of pro- and pre-B-cells. It promotes the autophosphorylation and activation of SYK, a key signaling molecule, by binding to BLNK and bringing it into proximity with SYK, allowing for the phosphorylation of BLNK. CD79A also interacts with certain Src-family tyrosine kinases, such as FYN and LYN, increasing their activity. Notably, it represses BCR signaling during the development of immature B-cells. CD79A forms a disulfide-linked heterodimer with CD79B, and together they constitute the B-cell antigen receptor complex, where the alpha/beta chain heterodimer is non-covalently associated with an antigen-specific membrane-bound surface immunoglobulin composed of two heavy chains and two light chains. CD79A interacts with SYK through its phosphorylated ITAM domain, facilitating SYK autophosphorylation and activation. Additionally, when phosphorylated on Tyr-210, CD79A interacts with the SH2 domain of BLNK/SLP65, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK, which is necessary for the trafficking of the BCR to late endosomes.

Species

Human

Source

HEK293

Tag

C-His;C-Flag

Accession

P11912 (L33-R143)&P40259 (A29-D159)

Gene ID

973  [NCBI]&974  [NCBI]

Synonyms
B-cell antigen receptor complex-associated protein; CD79a; IGA; MB1; CD79b; IGB
Molecular Weight

Approximately 37.5 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

CD79 Protein, Human (HEK293, Flag-His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CD79 Protein, Human (HEK293, Flag-His)
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HY-P75386
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