1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. GLO2/Glyoxalase II Protein, Human (GST)

GLO2/Glyoxalase II Protein, Human (GST)

Cat. No.: HY-P71690
Handling Instructions

GLO2, or Glyoxalase II Protein, serves as a thiolesterase, playing a crucial role in catalyzing the hydrolysis of S-D-lactoyl-glutathione. This activity generates glutathione and D-lactic acid, emphasizing GLO2's significance in cellular metabolism. The enzyme contributes to regulating glutathione levels and the conversion of substrates within cellular pathways. GLO2/Glyoxalase II Protein, Human (GST) is the recombinant human-derived GLO2/Glyoxalase II protein, expressed by E. coli , with N-GST labeled tag. The total length of GLO2/Glyoxalase II Protein, Human (GST) is 259 a.a., with molecular weight of ~55.7 kDa.

For research use only. We do not sell to patients.

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Description

GLO2, or Glyoxalase II Protein, serves as a thiolesterase, playing a crucial role in catalyzing the hydrolysis of S-D-lactoyl-glutathione. This activity generates glutathione and D-lactic acid, emphasizing GLO2's significance in cellular metabolism. The enzyme contributes to regulating glutathione levels and the conversion of substrates within cellular pathways. GLO2/Glyoxalase II Protein, Human (GST) is the recombinant human-derived GLO2/Glyoxalase II protein, expressed by E. coli , with N-GST labeled tag. The total length of GLO2/Glyoxalase II Protein, Human (GST) is 259 a.a., with molecular weight of ~55.7 kDa.

Background

GLO2, also known as Glyoxalase II, functions as a thiolesterase, playing a pivotal role in catalyzing the hydrolysis of S-D-lactoyl-glutathione. This enzymatic activity results in the formation of glutathione and D-lactic acid. The specific role of GLO2 in this process underscores its importance in the cellular metabolism of S-D-lactoyl-glutathione, contributing to the regulation of glutathione levels and the conversion of substrates involved in cellular pathways.

Species

Human

Source

E. coli

Tag

N-GST

Accession

Q16775 (50K-308D)

Gene ID
Molecular Construction
N-term
GST
GLO2 (50K-308D)
Accession # Q16775
C-term
Synonyms
HAGH; GLO2; HAGH1Hydroxyacylglutathione hydrolase; mitochondrial; EC 3.1.2.6; Glyoxalase II; Glx II
AA Sequence

KVEVLPALTDNYMYLVIDDETKEAAIVDPVQPQKVVDAARKHGVKLTTVLTTHHHWDHAGGNEKLVKLESGLKVYGGDDRIGALTHKITHLSTLQVGSLNVKCLATPCHTSGHICYFVSKPGGSEPPAVFTGDTLFVAGCGKFYEGTADEMCKALLEVLGRLPPDTRVYCGHEYTINNLKFARHVEPGNAAIREKLAWAKEKYSIGEPTVPSTLAEEFTYNPFMRVREKTVQQHAGETDPVTTMRAVRREKDQFKMPRD

Molecular Weight

Approximately 55.7 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

GLO2/Glyoxalase II Protein, Human (GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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GLO2/Glyoxalase II Protein, Human (GST)
Cat. No.:
HY-P71690
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