TPP1 Protein, Mouse (sf9, His)
TPP1, a lysosomal serine protease, acts as a non-specific lysosomal peptidase, displaying tripeptidyl-peptidase I activity in lysosomal protein degradation. Its function involves generating tripeptides from the breakdown products of other lysosomal proteinases, emphasizing its role in processing and hydrolyzing peptides within the lysosomal environment. Notably, TPP1 requires substrates with an unsubstituted N-terminus. TPP1 Protein, Mouse (sf9, His) is the recombinant mouse-derived TPP1 protein, expressed by Sf9 insect cells , with C-His labeled tag.
- Species: Mouse
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
TPP1, a lysosomal serine protease, acts as a non-specific lysosomal peptidase, displaying tripeptidyl-peptidase I activity in lysosomal protein degradation. Its function involves generating tripeptides from the breakdown products of other lysosomal proteinases, emphasizing its role in processing and hydrolyzing peptides within the lysosomal environment. Notably, TPP1 requires substrates with an unsubstituted N-terminus. TPP1 Protein, Mouse (sf9, His) is the recombinant mouse-derived TPP1 protein, expressed by Sf9 insect cells , with C-His labeled tag.
TPP1, a lysosomal serine protease, exhibits tripeptidyl-peptidase I activity, as evidenced by its involvement in lysosomal protein degradation. While its precise substrate specificity is not yet fully elucidated, TPP1 is thought to function as a non-specific lysosomal peptidase, generating tripeptides from the breakdown products produced by other lysosomal proteinases. Notably, TPP1 requires substrates with an unsubstituted N-terminus, further highlighting its role in processing and hydrolyzing peptides within the lysosomal environment.
Technical Parameters
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Species Mouse
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Source Sf9 insect cells
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Tag C-His
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Accession
O89023 (T20-P562)
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Molecular Construction
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N-term
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TPP1 (T20-P562)
Accession # O89023 -
His
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C-term
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Protein Length
Full Length of Mature Protein (with Propeptide)
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Synonyms
TPP1; Tripeptidyl Peptidase I; Prev. CLN2; Ceroid-Lipofuscinosis, Neuronal 2, Late Infantile (Jansky-Bielschowsky Disease); Prev. SCAR7; Spinocerebellar Ataxia, Autosomal Recessive 7; TPP-1; Lysosomal Pepstatin Insensitive Protease; LPIC; Lysosomal Pepsta
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AA Sequence
TYNPEPDQRWMLPPGWVSLGRVDPEEELSLTFALKQRNLERLSELVQAVSDPSSPQYGKYLTLEDVAELVQPSPLTLLTVQKWLSAAGARNCDSVTTQDFLTCWLSVRQAELLLPGAEFHRYVGGPTKTHVIRSPHPYQLPQALAPHVDFVGGLHRFPPSSPRQRPEPQQVGTVSLHLGVTPSVLRQRYNLTAKDVGSGTTNNSQACAQFLEQYFHNSDLTEFMRLFGGSFTHQASVAKVVGKQGRGRAGIEASLDVEYLMSAGANISTWVYSSPGRHEAQEPFLQWLLLLSNESSLPHVHTVSYGDDEDSLSSIYIQRVNTEFMKAAARGLTLLFASGDTGAGCWSVSGRHKFRPSFPASSPYVTTVGGTSFKNPFLITDEVVDYISGGGFSNVFPRPPYQEEAVAQFLKSSSHLPPSSYFNASGRAYPDVAALSDGYWVVSNMVPIPWVSGTSASTPVFGGILSLINEHRILNGRPPLGFLNPRLYQQHGTGLFDVTHGCHESCLNEEVEGQGFCSGPGWDPVTGWGTPNFPALLKTLLNP
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Predicted Molecular Mass
60.9 kDa
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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