Ristocetin A sulfate
Based on 1 Customer Validation
Ristocetin A (sulfate) (Ristomycin III) is a glycopeptide antibiotic that binds to von Willebrand factor (VWF) and bacterial cell wall components. Ristocetin A (sulfate) interferes with the biosynthesis of bacterial peptidoglycan by inhibiting transpeptidation. As an inducer of platelet adhesion and aggregation, Ristocetin A (sulfate) drives conformational changes by binding to the A1 domain of VWF, thereby activating downstream signaling pathways and promoting cytoskeletal rearrangement. Ristocetin A (sulfate) not only enhances platelet adhesion and spreading on immobilized VWF, but also induces the formation of asymmetric dimers with anticooperativity between platelets and plasma VWF. Ristocetin A (sulfate) is widely used in studies related to thromboembolic diseases and bacterial infections.
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- Pureté : 95.02%
- CAS No.: 92989-99-6
- Formule: C95H110N8O44·xH2SO4
- Masse moléculaire:2067.92 (free base)
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Stockage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
Description
In Vitro
Ristocetin A (sulfate) (1 mg/mL; 10 min, 45 min) induces the formation of intact, smooth lamellipodia and enhances the adhesion of washed human platelets to immobilized VWF; this effect relies on an αᵢᵢbβ3-dependent, GPIb-independent pathway, and requires PI3-kinase, Src-kinase and intracellular Ca2+ signaling, with an average platelet spreading surface area of 41.0 μm2[1].
Ristocetin A (ulfate) (1 mg/mL; 900 s) induces rapid, smooth lamellipodial protrusion of human washed platelets on immobilized VWF surfaces; this signaling pathway is dependent on αIIbβ3 and independent of GPIb[1].
Ristocetin A (sulfate) (1 mg/mL; 45 min) induces the formation of F-actin stress fibers in human washed platelets spread on immobilized VWF, and causes uniform localization of the Arp2/3 complex and talin [1].
Ristocetin A (sulfate) (1 mg/mL; 4 min) significantly enhances the adhesion and spreading of washed human platelets on immobilized VWF under flow conditions, with an average platelet spreading surface area of 40.4 μm2, and this process depends on the αiibβ3 pathway[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 92989-99-6
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Appearance Solid
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Masse moléculaire 2067.92 (free base)
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Formule C95H110N8O44·xH2SO4
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Color White to off-white
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SMILES
O=C(N[C@H](C(OC)=O)C1=C(C2=C(O)C=CC([C@@H](NC([C@H]3C4=CC(OC5=CC=C([C@@H](O)[C@@H]6C(N[C@H](C(N3)=O)C7=CC(O)=C(C)C(OC8=C(O)C=CC([C@@H](N)C(N6)=O)=C8)=C7)=O)C=C5)=C(O[C@H]9[C@H](O[C@@H]%10[C@@H](O[C@H]%11[C@@H](O)[C@H](O)[C@H](O)CO%11)[C@@H](O)[C@H](O)[C@@H](CO)O%10)[C@@H](O)[C@H](O)[C@@H](CO[C@H]%12[C@H](O)[C@H](O)[C@@H](O)[C@H](C)O%12)O9)C(OC%13=CC=C%14C=C%13)=C4)=O)C(N%15)=O)=C2)C(O[C@H]%16[C@@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O%16)=CC(O)=C1)[C@@H]%15[C@@H]%14O[C@H]%17C[C@@H](N)[C@@H](O)C(C)O%17.OS(O)(=O)=O.[x]
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Synonyms
Ristomycin III
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Protocole
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Phalloidin F-actin cytoskeleton staining
Phalloidin F-actin staining detects polymerized filamentous actin in fixed and permeabilized specimens by using fluorescent phalloidin or phalloidin-derived phallotoxins that bind actin filaments and generate a fluorescence microscopy readout corresponding to F-actin organization, including stress fibers, cortical actin, filament bundles, and tissue-specific actin networks. Phalloidin stabilizes F-actin by reducing actin subunit dissociation from filament ends, and fluorescent phallotoxins were established as tools for visualizing actin-containing structures in eukaryotic cells.
Pureté et documentation
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Fiche technique (310 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Ristocetin A sulfate Related Classifications
HY-138053 Related Classifications
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