RMR-Tre 

RMR-Tre is a fluorescent probe targeting the mycobacterial acyltransferase Ag85. Under the catalysis of Ag85, RMR-Tre undergoes 6-position mycoloylation and anchors to the mycobacterial membrane, while achieving fluorescence activation by inhibiting the intramolecular twisted charge transfer state transition. RMR-Tre can distinguish live mycobacteria from dead ones through metabolism-driven labeling, enabling rapid, wash-free, low-background detection of viable bacteria. RMR-Tre reports the drug resistance of Mycobacterium tuberculosis via the trehalose catalytic shift activity readout associated with TreS. In addition, RMR-Tre can be combined with flow cytometry or high-content imaging techniques to visualize and quantitatively analyze the metabolic heterogeneity of Mycobacterium tuberculosis related to persistence and drug resistance. RMR-Tre is widely used in tuberculosis-related research^[1][2].

RMR-Tre (1 μM-1 mM; 5 min-16 h) rapidly and efficiently labels live M. smegmatis in a time- and concentration-dependent manner without inhibiting bacterial growth, achieving >2000-fold fluorescence enhancement at 100 μM for 4 h^[1].
RMR-Tre (100 μM; 4 h) specifically labels live M. smegmatis via metabolically driven incorporation, enabling discrimination between live and dead M. smegmatis^[1].
RMR-Tre (100 μM; 30 min-4 h) incorporation into live M. smegmatis is primarily mediated by Ag85 mycoloyltransferase activity, as demonstrated by inhibition with Ebselen (HY-13750) and reduced labeling in Ag85-deficient mutant cells^[1].
RMR-Tre (100 μM; 4 h) exhibits high specificity for mycomembrane-containing bacterial species, efficiently labeling M. smegmatis and Corynebacterium glutamicum but not mycomembrane-deficient Escherichia coli or Bacillus subtilis^[1].
RMR-Tre (100 μM; 10 min-60 min) enables no-wash, rapid fluorescence microscopy labeling of live M. smegmatis, producing bright, localized fluorescence with low background as early as 10 min post-incubation^[1].
RMR-Tre enables visualization and quantification of metabolic heterogeneity linked to persistence and drug resistance in Mycobacterium tuberculosis, with high-label cell subpopulations correlating with elevated TreS activity, persister physiology, and enhanced antibiotic survival, and drug-resistant strains showing a larger baseline fraction of high-label cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

686.73

C₃₂H₃₈N₄O₁₁S

O[C@H]1[C@H](O)[C@@H](O)[C@@H](O[C@@H]2[C@H](O)[C@@H](O)[C@H](O)[C@@H](CNC(CCN3C4=C(C=C(C=C4)C5=CC=C(/C=C(C#N)/C#N)S5)CCC3)=O)O2)O[C@@H]1CO

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Banahene N, et al. A Far-Red Molecular Rotor Fluorogenic Trehalose Probe for Live Mycobacteria Detection and Drug-Susceptibility Testing. Angew Chem Int Ed Engl. 2023;62(2):e202213563.

  [2]. Lee J J, et al. Diagnostic Biomarker Candidate Discovery using Metabolomics of the Latent Mycobacterium tuberculosis[J]. Journal of Bacteriology and Virology, 2025, 55(4): 281-297.