RMR-Tre
RMR-Tre is a fluorescent probe targeting the mycobacterial acyltransferase Ag85. Under the catalysis of Ag85, RMR-Tre undergoes 6-position mycoloylation and anchors to the mycobacterial membrane, while achieving fluorescence activation by inhibiting the intramolecular twisted charge transfer state transition. RMR-Tre can distinguish live mycobacteria from dead ones through metabolism-driven labeling, enabling rapid, wash-free, low-background detection of viable bacteria. RMR-Tre reports the drug resistance of Mycobacterium tuberculosis via the trehalose catalytic shift activity readout associated with TreS. In addition, RMR-Tre can be combined with flow cytometry or high-content imaging techniques to visualize and quantitatively analyze the metabolic heterogeneity of Mycobacterium tuberculosis related to persistence and drug resistance. RMR-Tre is widely used in tuberculosis-related research.
For research use only. We do not sell to patients.
- Formula: C32H38N4O11S
- Molecular Weight:686.73
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
RMR-Tre (1 μM-1 mM; 5 min-16 h) rapidly and efficiently labels live M. smegmatis in a time- and concentration-dependent manner without inhibiting bacterial growth, achieving >2000-fold fluorescence enhancement at 100 μM for 4 h[1].
RMR-Tre (100 μM; 4 h) specifically labels live M. smegmatis via metabolically driven incorporation, enabling discrimination between live and dead M. smegmatis[1].
RMR-Tre (100 μM; 30 min-4 h) incorporation into live M. smegmatis is primarily mediated by Ag85 mycoloyltransferase activity, as demonstrated by inhibition with Ebselen (HY-13750) and reduced labeling in Ag85-deficient mutant cells[1].
RMR-Tre (100 μM; 4 h) exhibits high specificity for mycomembrane-containing bacterial species, efficiently labeling M. smegmatis and Corynebacterium glutamicum but not mycomembrane-deficient Escherichia coli or Bacillus subtilis[1].
RMR-Tre (100 μM; 10 min-60 min) enables no-wash, rapid fluorescence microscopy labeling of live M. smegmatis, producing bright, localized fluorescence with low background as early as 10 min post-incubation[1].
RMR-Tre enables visualization and quantification of metabolic heterogeneity linked to persistence and drug resistance in Mycobacterium tuberculosis, with high-label cell subpopulations correlating with elevated TreS activity, persister physiology, and enhanced antibiotic survival, and drug-resistant strains showing a larger baseline fraction of high-label cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Molecular Weight 686.73
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Formula C32H38N4O11S
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SMILES
O[C@H]1[C@H](O)[C@@H](O)[C@@H](O[C@@H]2[C@H](O)[C@@H](O)[C@H](O)[C@@H](CNC(CCN3C4=C(C=C(C=C4)C5=CC=C(/C=C(C#N)/C#N)S5)CCC3)=O)O2)O[C@@H]1CO
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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