RMR-Tre

RMR-Tre (1 μM-1 mM; 5 min-16 h) rapidly and efficiently labels live M. smegmatis in a time- and concentration-dependent manner without inhibiting bacterial growth, achieving >2000-fold fluorescence enhancement at 100 μM for 4 h^[1].
RMR-Tre (100 μM; 4 h) specifically labels live M. smegmatis via metabolically driven incorporation, enabling discrimination between live and dead M. smegmatis^[1].
RMR-Tre (100 μM; 30 min-4 h) incorporation into live M. smegmatis is primarily mediated by Ag85 mycoloyltransferase activity, as demonstrated by inhibition with Ebselen (HY-13750) and reduced labeling in Ag85-deficient mutant cells^[1].
RMR-Tre (100 μM; 4 h) exhibits high specificity for mycomembrane-containing bacterial species, efficiently labeling M. smegmatis and Corynebacterium glutamicum but not mycomembrane-deficient Escherichia coli or Bacillus subtilis^[1].
RMR-Tre (100 μM; 10 min-60 min) enables no-wash, rapid fluorescence microscopy labeling of live M. smegmatis, producing bright, localized fluorescence with low background as early as 10 min post-incubation^[1].
RMR-Tre enables visualization and quantification of metabolic heterogeneity linked to persistence and drug resistance in Mycobacterium tuberculosis, with high-label cell subpopulations correlating with elevated TreS activity, persister physiology, and enhanced antibiotic survival, and drug-resistant strains showing a larger baseline fraction of high-label cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.