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Reactive dye

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By Targets:	Fluorescent Dye (127) Reactive Oxygen Species (ROS) (6) Biochemical Assay Reagents (3) Cholinesterase (ChE) (1) DNA Stain (6) DNA/RNA Synthesis (1) MOFs (1) Reference Standards (1) VSV (1)
By Research Areas:	Cancer (6) Infection (1) Inflammation/Immunology (2)
Click Chemistry:	Labeling and Fluorescence Imaging (2) DBCO (1) Azide (4) Tetrazine (1) Alkynes (1)

138

Inhibitors & Agonists

124

Fluorescent Dye

Biochemical Assay Reagents

Click Chemistry

Targets Recommended: Fluorescent Dye Reactive Oxygen Species (ROS)

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-D0819

CY5-SE Maximum Cited Publications 37 Publications Verification Cy5 NHS Ester; Sulfo-Cyanine5 Succinimidyl Ester	Fluorescent Dye	Others
Cy5-SE (Cy5 NHS Ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reaction. This reagent is ideal for very cost-efficient labeling of soluble proteins, as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. Excitation (nm):649, Emission (nm): 670.

HY-138200

Cy5 maleimide 1 Publications Verification Cyanine5 maleimide	DNA Stain Fluorescent Dye	Others
Cy5 maleimide (Cyanine5 maleimide) is a single-reactive dye containing a maleimide group and a CY5 fluorescent group, which can selectively couple with a thiol group. Cy5 maleimide can be used to label antibodies, peptides, or proteins (Ex/Em = 646/664 nm).

HY-D0819A

CY5-SE triethylamine salt Maximum Cited Publications 37 Publications Verification Cy5 NHS Ester triethylamine salt; Sulfo-Cyanine5 Succinimidyl Ester triethylamine salt	Fluorescent Dye	Others
Cy5-SE (Cy5 NHS Ester) triethylamine salt is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. Cy5-SE triethylamine salt is ideal for very cost-efficient labeling of soluble proteins, as well as all kinds of peptides and oligonucleotides Ex=649 nm; Em=670 nm) .

HY-D0069

CPM	Fluorescent Dye	Others Cancer
CPM is a maleimide derivative, acting as a blue fluorescent thiol-reactive dye (excitation/emission maxima of 387/463 nm) .

HY-D0049

6-TAMRA-SE 1 Publications Verification 6-TAMRA-NHS ester; 6-Carboxytetramethylrhodamine N-succinimidyl ester	DNA Stain	Others
6-TAMRA-SE (6-TAMRA-NHS ester) is a fluorescent dye carrying the amine reactive group. 6-TAMRA-SE is one of the traditional fluorophores used for automated DNA sequencing .

HY-135415

Cyanine5.5 NHS ester chloride	Fluorescent Dye	Others
Cyanine5.5 NHS ester chloride is a reactive dye for the labeling of proteins, and antibodies and small molecular compounds .

HY-103469

Peroxy Orange 1 3 Publications Verification PO1	Fluorescent Dye	Others
Peroxy Orange 1 (PO1) is a new H₂O₂ specific probe that can bind to green fluorescent highly reactive oxygen species (hROS) probe APF. Peroxy Orange 1 is also a living cell dye .

HY-D0331

Reactive Blue 19	Biochemical Assay Reagents	Others
Reactive Blue 19 is an anthraquinone dye that can be used in the textile industry .

HY-125815

Reactive Blue 4 3 Publications Verification	Fluorescent Dye	Others
Reactive Blue 4 is an anthraquinone dye used extensively in the textile industry. Reactive Blue 4 is a single colorimetric chemosensor for sequential determination of multiple analytes with different optical responses in aqueous media .

HY-D1744

ICG Maleimide 1 Publications Verification	Fluorescent Dye	Others
ICG Maleimide is thiol reactive near infrared (NIR) fluorescent dye and used to generate a stable fluorescence signal in bioimaging (Ex/Em = 789/813 nm).

HY-D1605

BODIPY FL L-Cystine 1 Publications Verification	Fluorescent Dye	Others
BODIPY FL L-Cystine is a thiol-reactive, green-fluorescent dye. BODIPY FL L-Cystine can be the labeling of membrane proteins, proteins with hydrophobic binding sites, or hydrophobic ligands. (λ_ex=504 nm, λ_em=511 nm) .

HY-123749

Tetramethylrhodamine-5-iodoacetamide 5-TMRIA	Fluorescent Dye	Others
Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) is a thiol-selective reactive dye that is used to non-specifically label proteins via the cysteine residues. Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) can be used to covalently label DNA fragments .

HY-135413A

Sulfo-CY5-NHS ester potassium Sulfo-Cyanine5 NHS ester potassium	DNA Stain	Inflammation/Immunology Cancer
Sulfo-CY5-NHS ester potassium is a reactive dye and can be used for labeling protein nucleic acid .

HY-D0026

AMCA,SE 7-Amino-4-methyl-3-coumarinacetic acid N-succinimidyl ester	Fluorescent Dye	Others
AMCA,SE (7-Amino-4-methyl-3-coumarinacetic acid N-succinimidyl ester) is a bright blue fluorescent dye. AMCA,SE reacts with amino groups and is excited by ultraviolet light. AMCA,SE can be used for amine reactive labeling (Ex/Em = 354/440 nm) .

HY-D2746

Cy5.5 bis-NHS ester	Fluorescent Dye	Others
Cy5.5 bis-NHS ester is a reactive cyanine dye containing NHS ester which can be used to label amine groups in peptides, proteins, and oligonucleotides.

HY-D2173

AF488 azide	Fluorescent Dye	Others
AF488 azide is a fluorescent dye that can be used to determine the reactivity of immobilized DIBO groups. AF488 azide exhibits the λ_em and λ_ex wavelength of 520 nm and 470 nm, respectively .

HY-D1085

AMCA-X SE	Fluorescent Dye	Others
AMCA-X-SE is a coumarin derivative that generates fixed blue fluorescence and an NHS-activated ester that forms stable amide bonds with primary amine groups. It is used as a reactive dye for labeling amino groups of peptides, proteins, and oligonucleotides. Maximum excitation/emission wavelength: 354/442 nm .

HY-D1623

Cyanine3 carboxylic acid chloride	Fluorescent Dye	Others
Cyanine3 carboxylic acid chloride is a non-toxic red fluorescent dye with good solubility in organic solvents. Cyanine3 carboxylic acid chloride can be used as a non-reactive fluorophore for experimental control and calibration. Cyanine3 carboxylic acid chloride can also be synthesized with targeted agents as fluorescent probes for rapid detection of agent reactions .

HY-114351

BODIPY FL hydrazide	Fluorescent Dye	Others
BODIPY FL Hydrazide is a green-fluorescent dye, BODIPY FL Hydrazide is reactive with aldehyde/ketone on polysaccharides and glycoproteins, yielding a reversible Schiff base product that can be transformed to a stable linkage using a reducing agent like sodium borohydride or sodium cyanoborohydride. (λ_ex=495 nm, λ_em=516 nm) .

HY-DY1063

CY5-SE (solution)	Fluorescent Dye	Others
Cy5-SE (Cy5 NHS Ester) (solution) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reaction. This reagent is ideal for very cost-efficient labeling of soluble proteins, as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. Excitation (nm) :649, Emission (nm) : 670. Solvent and concentration: DMSO: 10 mM

HY-D1225

Reactive red 180	Fluorescent Dye
Reactive red 180 is common textile dyes that can be adsorbed onto single-walled carbon nanotubes (SWCNTs) through electrostatic interactions, allowing the separation of residual dyes.

HY-135413B

MeCY5-NHS ester triethylamine 1 Publications Verification Sulfo-Cyanine5 NHS ester triethylamine	DNA Stain	Others
MeCY5-NHS ester (potassium) is a reactive dye and can be used for labeling protein nucleic acid .

HY-D1319A

Cyanine5 carboxylic acid bromide Cy5 acid bromide	Fluorescent Dye	Others
Cyanine5 carboxylic acid (bromide) is a fluorescent dye containing a non-activated carboxylic acid (Ex=646 nm, Em=662 nm). Cyanine5 carboxylic acid chloride is an non-reactive dye that can be used in control samples .

HY-D1021

AA-dUTP sodium salt Aminoallyl-dUTP sodium salt	DNA/RNA Synthesis	Others
AA-dUTP (Aminoallyl-dUTP) sodium salt is a reverse transcriptase and DNA polymerase I substrate with probe precursor activity. AA-dUTP sodium salt undergoes enzymatic incorporation into DNA during cDNA synthesis and nick translation. AA-dUTP sodium salt generates amine-modified DNA, which can be used for labeling with amine-reactive fluorescent dyes .

HY-130736

Janelia Fluor® 549, SE JF549, SE; JF549, NHS	Fluorescent Dye	Others
Janelia Fluor® 549, SE (JF549, SE) is a cell-permeable fluorescent dye with an NHS ester (succinimidyl ester (SE)) reactive group. NHS esters react with primary amines and are commonly used to conjugate dyes to proteins, antibodies, amine-modified oligonucleotides, and other molecules. Janelia Fluor® 549, SE also acts as a ligand for self-labeling tags such as HaloTag and SNAP-tag, and can be used in live-cell imaging studies (Ex/Em = 549 nm/571 nm) .\n

HY-D1138

Reactive red 120	Fluorescent Dye
Reactive red 120 is common textile dyes that can be adsorbed onto single-walled carbon nanotubes (SWCNTs) through electrostatic interactions, allowing the separation of residual dyes.

HY-D0135

12-(7-Nitrobenzofurazan-4-ylamino)dodecanoic acid	Fluorescent Dye	Others
12-(7-Nitrobenzofurazan-4-ylamino)dodecanoic acid is an amine-reactive fluorescent dye .

HY-D0112

7-Hydroxycoumarin-4-acetic acid	Fluorescent Dye	Others
7-Hydroxycoumarin-4-acetic acid, a pH-indicator dye, is covalently bound to bovine serum albumin (BSA) at the positions of reactive amino groups (λ_max=326 nm) .

HY-D1372

Cyanine3 amine hydrochloride	Fluorescent Dye	Others
Cyanine3 amine hydrochloride, an analog of Cyanine3 amine, is a potent green fluorescent dye. Cyanine3 amine hydrochloride has the primary amine group and is covalently coupled with reactive groups such as NHS esters, carboxy groups (after carbodiimide activation), and epoxides. (λ_ex=555 nm, λ_em=570 nm) .

HY-D1372A

Cyanine3 amine TFA	Fluorescent Dye	Others
Cyanine3 amine (TFA), an analog of Cyanine3 amine, is a potent green fluorescent dye. Cyanine3 amine (TFA) has the primary amine group and is covalently coupled with reactive groups such as NHS esters, carboxy groups (after carbodiimide activation), and epoxides. (λ_ex=555 nm, λ_em=570 nm) .

HY-D2529

Cy5-PEG2000-SH	Fluorescent Dye	Others
Cy5-PEG2000-SH is a fluorescent labeling reagent that combines Cy5 (HY-D0821) fluorescent dye, polyethylene glycol (PEG) and sulfhydryl (SH) derivatives, and is suitable for molecular coupling. SH is highly reactive and can react with a variety of functional groups (such as maleimide) to form stable thioether bonds.

HY-D1886

Vari Fluor 647 SE	Fluorescent Dye	Others
Vari Fluor 647 SE is a fluorescent dye, SE stands for "succinimidyl ester". Vari Fluor 647 SE belongs to the Vari Fluor family of labeling reagents used in cell and molecular biology research. Vari Fluor 647 SE can react with an amino group to form a covalent bond, thereby introducing Vari Fluor 647 dye into the target molecule or cell. Vari Fluor 647 SE is a reactive dye that produces a fluorescent signal after binding to a target molecule or cell.

HY-D0374

Reactive Blue 4 sodium	Fluorescent Dye	Others
Reactive Blue 4 sodium is an anthraquinone dye used extensively in the textile industry. Reactive Blue 4 sodium is a single colorimetric chemosensor for sequential determination of multiple analytes with different optical responses in aqueous media .

HY-W998687

Cy5 dimethyl	Fluorescent Dye	Others
Cy5 dimethyl is a free non-activated dye. This dye has limited water solubility, but can be dissolved in mixtures of water with organic phase (DMF, DMSO, alcohols) to obtain useful concentrations of the material in solution. This molecule can be considered non-reactive dye for the use in control samples, and for instrument calibration.

HY-D2497

Sulfo Cy7 bis-SH	Fluorescent Dye	Others
Sulfo Cy7 bis-SH is a fluorescent dye that contains sulfonated Cyanine7 (Sulfo-CY7) fluorescent dye and thiol (-SH) functional group. SH is highly reactive and can react with a variety of functional groups (such as maleimide) to form stable thioether bonds .

HY-W998707

TAMRA amine, 5-isomer hydrochloride	Fluorescent Dye	Others
TAMRA amine, 5-isomer hydrochloride is a rhodamine dye with excitation/emission maximum 553/575 nm. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc.

HY-W927359

Fluorescein hydrazide (3,6-Dihydroxyxanthane)	Fluorescent Dye	Others
Fluorescein hydrazide (3,6-Dihydroxyxanthane) is a fluorescein dye that can be used for the fluorophotometric determination of hydrogen peroxide (H2O2) and other reactive oxygen species (ROS) .

HY-D2750

Sulfo-Cy5 picolyl azide	Fluorescent Dye	Others
Sulfo-Cy5 Picolyl Azide is a fluorophore featuring a sulfonate group and an azide. Azide groups are click chemistry handles which are reactive towards terminal alkynes and strained cyclooctynes such as BCN or DBCO. Cy5 is a cyanine dye with excitation and emission maxima at 651 nm and 670 nm respectively. The sulfonate group on the Cy5 dye increases this compound’s water solubility.

HY-172721

Cy3B maleimide	Fluorescent Dye	Others
Cy3B maleimide is a mono-reactive dye containing maleimide group, which can selectively and efficiently attach Cyanine3 fluorophore (an analog of Cy3) to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides). Cy3B is an improved version of Cy3 dyes with significantly increased fluorescence quantum yield and photostability.

HY-D1352A

Sulfo-Cyanine7 NHS ester TEA	Fluorescent Dye	Others
Sulfo-Cyanine7 NHS ester (TEA) is an amine-reactive succinimide ester. Sulfo-Cyanine7 NHS ester (TEA) reagent allows to prepare sulfo-Cyanine7-labeled biomolecules, such as proteins, with ease. Dye labeled molecules can be subsequently used for various research and agent design related experiments.

HY-D1596A

Cyanine 3.5 bromide Cy3.5 NHS ester bromide; Cy 3.5 bromide	Fluorescent Dye	Others
Cyanine 3.5 bromide (Cy3.5 NHS ester bromide) is an analog of the Cy3.5 fluorophore. Cyanine 3.5 bromide is a red reactive fluorescent dye. Cyanine 3.5 bromide is used to label amino groups in peptides, proteins, and oligonucleotides. (λ_ex=591 nm, λ_em=604 nm) .

HY-D2531

Cy5-PEG5000-SH	Fluorescent Dye	Others
Cy5-PEG5000-SH is a fluorescent labeling reagent that combines Cy5 (HY-D0821) fluorescent dye, polyethylene glycol (PEG) and sulfhydryl (SH) derivatives, and is suitable for molecular coupling. SH is highly reactive and can react with a variety of functional groups (such as maleimide) to form stable thioether bonds.

HY-151713

Sulfo-Cy3-Methyltetrazine	Fluorescent Dye	Others
Sulfo-Cy3-Methyltetrazine (figure 7 compound 5) is a click chemistry reagent containing methyltetrazine and a water-soluble dye. Sulfo-Cy3-Methyltetrazine shows good stability at physiological pH. Sulfo-Cy3-Methyltetrazine can be coupled to trans-cyclooctene and is also highly reactive towards cyclooctene .

HY-D0661

Reactive red 45	Fluorescent Dye
Reactive red 45 is common textile dyes that can be adsorbed onto single-walled carbon nanotubes (SWCNTs) through electrostatic interactions, allowing the separation of residual dyes.

HY-D0622

Reactive Green 19	Fluorescent Dye
Reactive Green 19 is common textile dyes that can be adsorbed onto single-walled carbon nanotubes (SWCNTs) through electrostatic interactions, allowing the separation of residual dyes.

HY-D0514

Reactive blue 5	Fluorescent Dye
Reactive blue 5 is common textile dyes that can be adsorbed onto single-walled carbon nanotubes (SWCNTs) through electrostatic interactions, allowing the separation of residual dyes.

HY-D1309

Sulfo-cyanine3 NHS ester sodium	Fluorescent Dye
Sulfo-cyanine3 NHS ester sodium is a water-soluble cyanine dye that contains NHS ester groups and is amino-reactive.

HY-D1611

BODIPY FL-C5 NHS Ester	Fluorescent Dye	Others
BODIPY FL-C5 NHS Ester is a green-fluorescent dye that is reactive with primary amines on biomolecules such as peptides, proteins, modified nucleotides and biopolymers .

HY-D0655

Reactive red 24	Fluorescent Dye	Others
Reactive red 24 is a multifunctional dye. Dyes are important tools in biological experiments. They can help researchers observe and analyze cell structures, track biomolecules, evaluate cell functions, distinguish cell types, detect biomolecules, study tissue pathology and monitor microorganisms. Their applications range from basic scientific research to clinical A wide range of diagnostics. Dyes are also widely used in traditional fields such as textile dyeing, as well as in emerging fields such as functional textile processing, food pigments and dye-sensitized solar cells.

HY-D0634

Reactive Black 39	Fluorescent Dye	Others
Reactive Black 39 is a multifunctional dye. Dyes are important tools in biological experiments. They can help researchers observe and analyze cell structures, track biomolecules, evaluate cell functions, distinguish cell types, detect biomolecules, study tissue pathology and monitor microorganisms. Their applications range from basic scientific research to clinical A wide range of diagnostics. Dyes are also widely used in traditional fields such as textile dyeing, as well as in emerging fields such as functional textile processing, food pigments and dye-sensitized solar cells.

Cat. No.	Product Name	Type

HY-15930B

TMB dihydrochloride x.hydrate

5+ Cited Publications

Sure Blue TMB dihydrochloride x.hydrate

Biochemical Assay Reagents

TMB (dihydrochloride x.hydrate) is the dihydrochloride x hydrate form of TMB (HY-15930). TMB (dihydrochloride x.hydrate) is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB (dihydrochloride x.hydrate) can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB (dihydrochloride x.hydrate) can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922 .

HY-D1021

AA-dUTP sodium salt Aminoallyl-dUTP sodium salt	Biochemical Assay Reagents
AA-dUTP (Aminoallyl-dUTP) sodium salt is a reverse transcriptase and DNA polymerase I substrate with probe precursor activity. AA-dUTP sodium salt undergoes enzymatic incorporation into DNA during cDNA synthesis and nick translation. AA-dUTP sodium salt generates amine-modified DNA, which can be used for labeling with amine-reactive fluorescent dyes .

HY-D0015

Cresol red, 97%

Biochemical Assay Reagents

Cresol red, 97% is an acidic phenolsulfonphthalein triphenylmethane dye. Cresol red, 97% generates reactive oxygen species and singlet oxygen under ultrasonic irradiation, interacts with bovine serum albumin, alters the structure of bovine serum albumin, and oxidizes the fluorescent amino acid residues of bovine serum albumin. Cresol red, 97% can be decolorized and biodegraded by *Absidia spinosa* M15, producing phenylacetic acid and benzoic acid metabolites. Cresol red, 97% serves as a pH indicator (orange at pH 1.8-yellow at pH 2.0, yellow at pH 7.0-purple at pH 8.8). Cresol red, 97% can be used to indicate pH, temperature and salinity .

Cat. No.	Product Name		Classification

HY-D1583

Cyanine5 DBCO hexafluorophosphate 4 Publications Verification DBCO-Cy5 hexafluorophosphate; Cyanine5 dibenzocyclooctyne hexafluorophosphate		DBCO
Cyanine5 DBCO (DBCO-Cy5) hexafluorophosphate is a low-toxicity azide reactive probe (NIR fluorescent dye), for imaging azide-labeled biomolecules via a copper-free "click-through" reaction. Cyanine5 DBCO hexafluorophosphate has no apparent cytotoxicity or animal toxicity and shows no damage to the physiological functions of cells other than the target cells (azide-labeled cells). Cyanine5 DBCO hexafluorophosphate can be used to label and track cells in vitro and in vivo (Ex=635 nm, Em=650-700 nm) .

HY-D2746

Cy5.5 bis-NHS ester		Azide
Cy5.5 bis-NHS ester is a reactive cyanine dye containing NHS ester which can be used to label amine groups in peptides, proteins, and oligonucleotides.

HY-D2173

AF488 azide		Azide
AF488 azide is a fluorescent dye that can be used to determine the reactivity of immobilized DIBO groups. AF488 azide exhibits the λ_em and λ_ex wavelength of 520 nm and 470 nm, respectively .

HY-D0820

CY5-YNE Sulfo-Cyanine5-alkyne		Alkynes Labeling and Fluorescence Imaging
CY5-YNE (Sulfo-Cyanine5-alkyne) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides (Ex: 650 nM; Em: 680 nm). CY5-YNE is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

HY-D2750

Sulfo-Cy5 picolyl azide		Azide
Sulfo-Cy5 Picolyl Azide is a fluorophore featuring a sulfonate group and an azide. Azide groups are click chemistry handles which are reactive towards terminal alkynes and strained cyclooctynes such as BCN or DBCO. Cy5 is a cyanine dye with excitation and emission maxima at 651 nm and 670 nm respectively. The sulfonate group on the Cy5 dye increases this compound’s water solubility.

HY-151713

Sulfo-Cy3-Methyltetrazine		Tetrazine Labeling and Fluorescence Imaging
Sulfo-Cy3-Methyltetrazine (figure 7 compound 5) is a click chemistry reagent containing methyltetrazine and a water-soluble dye. Sulfo-Cy3-Methyltetrazine shows good stability at physiological pH. Sulfo-Cy3-Methyltetrazine can be coupled to trans-cyclooctene and is also highly reactive towards cyclooctene .

HY-D2740

ROX azide, 6-isomer		Azide
ROX azide, 6-isomer is an alkyne-reactive derivative of ROX (Rhodamine X, Rhodamine 101) dye. ROX is a red-emitting fluorophore possessing high brightness and fluorescence quantum yield. This reagent is a pure 6-isomer. It is used for labeling alkyne and cycloalkyne-containing biomolecules via copper-catalyzed and copper-free click chemistry reactions.

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