Tocilizumab
Based on 43 publication(s) in Google Scholar
Tocilizumab (Anti-Human IL6R, Humanized Antibody) is an anti-human interleukin-6 receptor (IL-6R) neutralizing antibody, prevents binding of IL-6 to the IL-6R, thereby inhibiting both classic and trans-signaling. Tocilizumab (Anti-Human IL6R, Humanized Antibody) can be used for the treatment of rheumatoid arthritis. Tocilizumab is remarkablely effective for the study of severe COVID-19 (coronavirus disease).
For research use only. We do not sell to patients.
- Purity: 99.47%
- CAS No.: 375823-41-9
- Molecular Weight:145.2 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Tocilizumab
More- ACS Nano. 2025 Sep 16;19(36):32361-32373. [Abstract]
- ACS Nano. 2025 Mar 24. [Abstract]
- Adv Sci (Weinh). 2025 Oct 28:e16569. [Abstract]
- Adv Sci (Weinh). 2024 Dec 11:e2407398. [Abstract]
- J Adv Res. 2026 Feb 16:S2090-1232(26)00158-X. [Abstract]
- J Biomed Sci. 2022 Nov 24;29(1):101. [Abstract]
- Redox Biol. 2025 Apr:81:103557. [Abstract]
- Cell Mol Biol Lett. 2025 Mar 29;30(1):37. [Abstract]
- Cancer Lett. 2025 Jul 31:632:217964. [Abstract]
- Int J Biol Sci. 2024 Aug 6;20(11):4222-4237. [Abstract]
- Cell Death Dis. 2021 Dec 20;13(1):16. [Abstract]
- Cell Commun Signal. 2025 Apr 23;23(1):194. [Abstract]
- Phytomedicine. 2023 Mar:111:154659. [Abstract]
- Sci Total Environ. 2024 Jun 24:946:174202. [Abstract]
- Mol Med. 2024 Oct 10;30(1):171. [Abstract]
- Cell Mol Life Sci. 2025 Feb 22;82(1):85. [Abstract]
- Cell Mol Life Sci. 2024 May 28;81(1):240. [Abstract]
- Biochem Pharmacol. 2023 Jul:213:115618. [Abstract]
- Breast Cancer Res. 2023 Apr 17;25(1):43. [Abstract]
- Pharmaceutics. 2025 Jul 21;17(7):945. [Abstract]
- Inflamm Res. 2025 Apr 29;74(1):72. [Abstract]
- Int J Mol Sci. 2024 Nov 14;25(22):12212. [Abstract]
- Endocr Relat Cancer. 2023 Jul 25;30(9):e230130. [Abstract]
- Biochim Biophys Acta Mol Basis Dis. 2026 Apr;1872(4):168171. [Abstract]
- Bioconjug Chem. 2025 Aug 21. [Abstract]
- Sci Rep. 2025 Jul 23;15(1):26808. [Abstract]
- Sci Rep. 2023 Aug 14;13(1):13231. [Abstract]
- Sci Rep. 2022 Jan 20;12(1):1075. [Abstract]
- Toxicol Appl Pharmacol. 2022 Apr 1:440:115921. [Abstract]
- Acta Biochim Biophys Sin (Shanghai). 2021 Dec 8;53(12):1670-1680. [Abstract]
- World J Clin Oncol. 2025 Oct 24;16(10):112097. [Abstract]
- Mol Carcinog. 2024 Sep;63(9):1827-1841. [Abstract]
- Int J Chron Obstruct Pulmon Dis. 2023 Apr 13:18:575-591. [Abstract]
- Mol Immunol. 2026 Apr:192:1-12. [Abstract]
- Oral Dis. 2024 May;30(4):2097-2109. [Abstract]
- Carcinogenesis. 2023 Jun 24;44(4):356-367. [Abstract]
- J Biochem Mol Toxicol. 2024 Jun;38(6):e23743. [Abstract]
- Biochem Biophys Res Commun. 2023 Apr 23:653:21-30. [Abstract]
- Scand J Immunol. 2023 Jul;98(1):e13271. [Abstract]
- bioRxiv. 2026 Apr 14.
- SSRN. 2024 Feb 7.
- Research Square Print. October 3rd, 2022.
- Universidade de São Paulo. 2020 Sep.
Customer Validation & Images
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Cell Imaging/Staining
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ELISA
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ELISA
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IF
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RT-PCR
Biological Activity
Human IgG1 kappa
Human
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IL-6Ra |
Tocilizumab (Anti-Human IL6R, Humanized Antibody) (1-100 ng/mL; 24 hours, 48 hours) inhibits the cell proliferation (growth rate) in a dose-dependent manner, growth rate by HACM is reduced almost completely by 100 ng/mL tocilizumab[2]. Tocilizumab (Anti-Human IL6R, Humanized Antibody) (0.001-10,000 μg/ml; 2 days) inhibits Ba/F3-gp130-IL-6R cell proliferation stimulated with hIL-6 with an IC50 of 13.5 ng/ml[1]. Tocilizumab (Anti-Human IL6R, Humanized Antibody) (1 μM; 6 hours) reduces the phosphorylation of STAT3 in vitro consistent with in vivo SAS cells[3]. Tocilizumab (Anti-Human IL6R, Humanized Antibody) (1-5 μM; 24 hours) significantly reduces VEGF mRNA expression in SAS cells with serum-free medium[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human-derived HARA-B cells
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Concentration:1 ng/mL, 10 ng/mL, and 100 ng/mL
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Incubation Time:24 hours, 48 hours
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Result:Inhibited the IL-6R expressed on human-derived HARA-B cells.
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Cell Line:Ba/F3-gp130-IL-6R cells
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Concentration:0.001–10000 μg/ml
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Incubation Time:2 days
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Result:Inhibited Ba/F3-gp130-IL-6R cell proliferation.
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Cell Line:SAS cells
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Concentration:1 μM
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Incubation Time:6 hours
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Result:Reduced the phosphorylation of STAT3.
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Cell Line:SAS cells
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Concentration:1-5 μM
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Incubation Time:24 hours
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Result:Reduced VEGF mRNA expression.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:A brain metastasis model of lung tumor cells by inoculating HARA-B cells in nude mice[2]
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Dosage:1.0 mg/100 μL
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Administration:Intravenous injection; 1.0 mg/100 μL; twice a week, three weeks
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Result:Led the volume of each metastatic focus significantly smaller.
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Animal Model:SCID mice with SAS cells[3]
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Dosage:100 μg/mL
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Administration:Intraperitoneal injection;100 μg/mL; every 48 hours; 20days
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Result:Suppressed the growth of advanced tumors.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
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Human IgG1 kappa
ELISA, FACS, Functional assay
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Immobilized Human IL-6Ra/CD126, His Tag can bind Tocilizumab. The EC50 for this effect is 30.32 ng/mL. -
Flow cytometry analysis of IL-6R expression U937 cells labelling IL-6R with Tocilizumab at 10nM (Red), 20ng/mL of FcγRI (CD64), FcγRII (CD32), and FcγRIII (CD16) were added to block the binding of Fc of antibody to cells respectively. Goat anti human IgG Fc(Alexa Fluor488) was used as the secondary antibody at 1/400 dilution. Isotype control - human IgG (Blue). Unlabeled control - Unlabelled cells (Gray).
Chemical Information
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CAS No. 375823-41-9
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Appearance Liquid
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Molecular Weight 145.2 kDa
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Color Colorless to light yellow
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SMILES
[Tocilizumab]
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Synonyms
Anti-Human IL6R, Humanized Antibody; RG-1569; rhPM-1
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (43)
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Journal Impact Factor
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Most Recent
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ACS Nano
Combining Microfluidic Assessment with Encoded Hydrogel Microparticle-Based Immunoassay for Anti-Interleukin Drug Repurposing of Pulmonary Fibrosis Therapy. [Abstract]2025 Sep 16;19(36):32361-32373. PMID: 40903905
Tocilizumab purchased from MedChemExpress. Usage Cited in: ACS Nano. 2025 Sep 16;19(36):32361-32373. [Abstract]
Representative images of fibrosis-induced spheroids subjected to microfluidic deformation analysis, comparing untreated control, antifibrotic drug NTD, and anti-interleukin drugs TCZ, STX, DUP, and TRA. Mab (5 μg/mL, 1 d) were added, Scale bars indicate 100 μm.
Tocilizumab purchased from MedChemExpress. Usage Cited in: ACS Nano. 2025 Sep 16;19(36):32361-32373. [Abstract]
IL-6 concentrations of fibrosis-induced spheroids measured in culture supernatants following drug treatments. Tocilizumab (TCZ, 5 μg/mL, 1 d) were added.
Tocilizumab purchased from MedChemExpress. Usage Cited in: ACS Nano. 2025 Sep 16;19(36):32361-32373. [Abstract]
TGF-β concentrations of fibrosis-induced spheroids measured in culture supernatants following drug treatments. Tocilizumab (TCZ, 5 μg/mL, 1 d) were added.
Tocilizumab purchased from MedChemExpress. Usage Cited in: ACS Nano. 2025 Sep 16;19(36):32361-32373. [Abstract]
Confocal fluorescence images showing the expression of collagen-1 and α-SMA in spheroids treated with NTD, TCZ, STX, DUP, and TRA. Mab (5 μg/mL, 1 d) were added, Scale bars indicate 100 μm.
Tocilizumab purchased from MedChemExpress. Usage Cited in: ACS Nano. 2025 Sep 16;19(36):32361-32373. [Abstract]
Relative mRNA expression level of α-SMA in spheroids treated with NTD and TCZ, normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression (n = 3). Tocilizumab (TCZ, 5 μg/mL, 1 d) were added.
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ACS Nano
High-Electron-Mobility MXene-Enhanced Metasurface Biosensors Integrated with Microfluidics for Real-Time Multifunctional Monitoring. [Abstract]2025 Mar 24. PMID: 40126942 -
Adv Sci (Weinh)
Repurposing of Chemokine Antagonists for Combined Phase-Resolved Spinal Cord Injury Treatment. [Abstract]2025 Oct 28:e16569. PMID: 41147460 -
Adv Sci (Weinh)
MAM-STAT3-Driven Mitochondrial Ca+2 Upregulation Contributes to Immunosenescence in Type A Mandibuloacral Dysplasia Patients. [Abstract]2024 Dec 11:e2407398. PMID: 39661729 -
J Adv Res
Tocilizumab-loaded nanoparticles block IL-6R and reduce edema after intracerebral hemorrhage. [Abstract]2026 Feb 16:S2090-1232(26)00158-X. PMID: 41707962 -
J Biomed Sci
Circadian gene Rev-erbα influenced by sleep conduces to pregnancy by promoting endometrial decidualization via IL-6-PR-C/EBPβ axis. [Abstract]2022 Nov 24;29(1):101. PMID: 36419076 -
Redox Biol
Quercetin alleviates LPS/iE-DAP-induced liver injury by suppressing ferroptosis via regulating ferritinophagy and intracellular iron efflux. [Abstract]2025 Apr:81:103557. PMID: 39986118 -
Cell Mol Biol Lett
CircNF1 modulates the progression and immune evasion of esophageal squamous cell carcinoma through dual regulation of PD-L1. [Abstract]2025 Mar 29;30(1):37. PMID: 40158127 -
Cancer Lett
Bacteria and tumor debris induced pancreatic cancer progression via the NF-κB signaling pathway. [Abstract]2025 Jul 31:632:217964. PMID: 40752749 -
Int J Biol Sci
IL-6 signaling accelerates iron overload by upregulating DMT1 in endothelial cells to promote aortic dissection. [Abstract]2024 Aug 6;20(11):4222-4237. PMID: 39247821 -
Cell Death Dis
Colorectal cancer-associated fibroblasts promote metastasis by up-regulating LRG1 through stromal IL-6/STAT3 signaling. [Abstract]2021 Dec 20;13(1):16. PMID: 34930899 -
Cell Commun Signal
CDO1 phosphorylation is required for IL-6-induced tumor cell proliferation through governing cysteine availability. [Abstract]2025 Apr 23;23(1):194. PMID: 40269955 -
Phytomedicine
YY1 was indispensable for the alleviation of quercetin on diabetic nephropathy-associated tubulointerstitial inflammation. [Abstract]2023 Mar:111:154659. PMID: 36641979 -
Sci Total Environ
Exposure to coal dust exacerbates cognitive impairment by activating the IL6/ERK1/2/SP1 signaling pathway. [Abstract]2024 Jun 24:946:174202. PMID: 38925396 -
Mol Med
Acetylcholine receptor-β inhibition by interleukin-6 in skeletal muscles contributes to modulating neuromuscular junction during aging. [Abstract]2024 Oct 10;30(1):171. PMID: 39390392 -
Cell Mol Life Sci
Inhibition of DYRK1B BY C81 impedes inflammatory processes in leukocytes by reducing STAT3 activity. [Abstract]2025 Feb 22;82(1):85. PMID: 39985685 -
Cell Mol Life Sci
Disruption of pulmonary microvascular endothelial barrier by dysregulated claudin-8 and claudin-4: uncovered mechanisms in porcine reproductive and respiratory syndrome virus infection. [Abstract]2024 May 28;81(1):240. PMID: 38806818 -
Biochem Pharmacol
2023 Jul:213:115618. PMID: 37211172 -
Breast Cancer Res
Targeting DCLK1 attenuates tumor stemness and evokes antitumor immunity in triple-negative breast cancer by inhibiting IL-6/STAT3 signaling. [Abstract]2023 Apr 17;25(1):43. PMID: 37069669 -
Pharmaceutics
Dual-Action Tocilizumab-Conjugated Cisplatin Nanoparticles Overcome Chemoresistance and Metastasis in Non-Small-Cell Lung Cancer. [Abstract]2025 Jul 21;17(7):945. PMID: 40733153 -
Inflamm Res
The IL-6 autocrine loop promoting IFN-γ-induced fibroblast senescence is involved in psychological stress-mediated exacerbation of vitiligo. [Abstract]2025 Apr 29;74(1):72. PMID: 40299037 -
Int J Mol Sci
Interleukin-6 Modulates the Expression and Function of HCN Channels: A Link Between Inflammation and Atrial Electrogenesis. [Abstract]2024 Nov 14;25(22):12212. PMID: 39596280 -
Endocr Relat Cancer
2023 Jul 25;30(9):e230130. PMID: 37260320 -
Biochim Biophys Acta Mol Basis Dis
Multimodal psychological intervention improves psychological well-being and inflammatory profiles in patients with diabetic nephropathy. [Abstract]2026 Apr;1872(4):168171. PMID: 41580211 -
Bioconjug Chem
Resin-Supported Site-Specific Antibody Conjugation Method Leads to Antibody-Drug Conjugates with Retained Efficacy and Improved Stability. [Abstract]2025 Aug 21. PMID: 40839643 -
Sci Rep
Berberine hydrochloride inhibits the proliferation and metastasis of p53 mutant gallbladder Cancer cells by regulating the IL6/STAT3 pathway. [Abstract]2025 Jul 23;15(1):26808. PMID: 40702096 -
Sci Rep
Interleukin 6 exacerbates the progression of warm autoimmune hemolytic anemia by influencing the activity and function of B cells. [Abstract]2023 Aug 14;13(1):13231. PMID: 37580421 -
Sci Rep
Arrhythmogenic mechanisms of interleukin-6 combination with hydroxychloroquine and azithromycin in inflammatory diseases. [Abstract]2022 Jan 20;12(1):1075. PMID: 35058480 -
Toxicol Appl Pharmacol
Indoleamine 2,3-dioxygenase 1 regulates breast cancer tamoxifen resistance through interleukin-6/signal transducer and activator of transcription. [Abstract]2022 Apr 1:440:115921. PMID: 35157906 -
Acta Biochim Biophys Sin (Shanghai)
Stattic sensitizes osteosarcoma cells to epidermal growth factor receptor inhibitors via blocking the interleukin 6-induced STAT3 pathway. [Abstract]2021 Dec 8;53(12):1670-1680. PMID: 34693451 -
World J Clin Oncol
Overcoming chemoresistance in non-small cell lung cancer: Insights into the influence of inflammatory factors on treatment response. [Abstract]2025 Oct 24;16(10):112097. PMID: 41178924 -
Mol Carcinog
LAIR1 promotes hepatocellular carcinoma cell metastasis and induces M2-macrophage infiltration through activating AKT-IKKβ-p65 axis. [Abstract]2024 Sep;63(9):1827-1841. PMID: 39016636 -
Int J Chron Obstruct Pulmon Dis
MicroRNA Let-7 Induces M2 Macrophage Polarization in COPD Emphysema Through the IL-6/STAT3 Pathway. [Abstract]2023 Apr 13:18:575-591. PMID: 37077365 -
Mol Immunol
Adenovirus induces pediatric pneumonia via triggering macrophage polarization and IL-6 production via NF-κB activation. [Abstract]2026 Apr:192:1-12. PMID: 41762478 -
Oral Dis
Mesenchymal stem cells-derived IL-6 promotes invasion and metastasis of oral squamous cell carcinoma via JAK-STAT3 signalling. [Abstract]2024 May;30(4):2097-2109. PMID: 37249062 -
Carcinogenesis
Exosome-transmitted miR-29a induces colorectal cancer metastasis by destroying the vascular endothelial barrier. [Abstract]2023 Jun 24;44(4):356-367. PMID: 36939367 -
J Biochem Mol Toxicol
UBE2T mediates SORBS3 ubiquitination to enhance IL-6/STAT3 signaling and promote lung adenocarcinoma progression. [Abstract]2024 Jun;38(6):e23743. PMID: 38816989 -
Biochem Biophys Res Commun
Hypoxia-inducible factor 1α/IL-6 axis in activated hepatic stellate cells aggravates liver fibrosis. [Abstract]2023 Apr 23:653:21-30. PMID: 36848821 -
Scand J Immunol
IL-6 upregulates the expression of IL-6R through the JAK2/STAT3 signalling pathway to promote progression of hepatocellular carcinoma. [Abstract]2023 Jul;98(1):e13271. PMID: 38441314 -
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Purity & Documentation
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Data Sheet (266 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Garbers C, et al. Inhibition of classic signaling is a novel function of soluble glycoprotein 130 (sgp130), which is controlled by the ratio of interleukin 6 and soluble interleukin 6 receptor. J Biol Chem. 2011 Dec 16;286(50):42959-70. [Content Brief]
[2]. Mami Noda, et al. IL-6 Receptor Is a Possible Target against Growth of Metastasized Lung Tumor Cells in the Brain. Int J Mol Sci. 2013 Jan; 14(1): 515–526. [Content Brief]
[3]. Shinriki S, et al. Humanized anti-interleukin-6 receptor antibody suppresses tumor angiogenesis and in vivogrowth of human oral squamous cell carcinoma. Clin Cancer Res. 2009 Sep 1;15(17):5426-34. [Content Brief]
[4]. Chi Zhang, et al. Cytokine Release Syndrome in Severe COVID-19: Interleukin-6 Receptor Antagonist Tocilizumab May Be the Key to Reduce Mortality. Int J Antimicrob Agents. 2020 May;55(5):105954. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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