USP25/28-IN-2
USP25/28-IN-2 is a selectivity dual USP25/28 inhibitor with IC50 values of 0.88 and 1.1 μM, respectively. USP25/28-IN-2 modulates USP25 and USP28 activity to affect downstream pathways, modulates c-Myc oncoprotein total levels and half-life, induces apoptosis, reduces cell viability. USP25/28-IN-2 can be used for the research of colorectal carcinoma, colorectal adenocarcinoma.
For research use only. We do not sell to patients.
- CAS No.: 2165322-95-0
- Formula: C17H17BrF3NO3
- Molecular Weight:420.23
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
USP25/28-IN-2 (compound AZ2) (0.01-60 μM; 2 h) engages endogenous USP28 in HCT116 cells, with an EC50 of 18.2 μM as measured by Ub-VS probe competition[1].
USP25/28-IN-2 (0.01-60 μM; 2 h) engages endogenous USP25 in HCT116 cells, with an EC50 of 11.5 μM as measured by Ub-VS probe competition[1].
USP25/28-IN-2 (0-100 μM; 3 h) dose-dependently reduces endogenous total c-Myc protein levels in HCT116 cells after 3 h of treatment[1].
USP25/28-IN-2 (0-100 μM; 3 h) dose-dependently induces apoptosis in HCT116 cells, as measured by PARP cleavage[1].
USP25/28-IN-2 (72 h) exerts dose-dependent anti-proliferative effects in multiple cancer cell lines (including HCT116, HT29, and SW480) and tissue-matched normal cell lines, with EC50 values clustered around 20 μM in cancer cells and 28 μM in normal cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:HCT116 colon carcinoma cells
-
Concentration:0; 20; 40; 60; 80; 100 μM
-
Incubation Time:3 h
-
Result:Dose-dependently reduced endogenous total c-Myc protein levels, with complete reduction observed at the highest concentrations tested.
Left USP28 protein levels unchanged (confirming no general protein degradation).
-
Cell Line:HCT116 colon carcinoma cells
-
Concentration:0; 20; 40; 60; 80; 100 μM
-
Incubation Time:3 h
-
Result:Dose-dependently induced PARP cleavage, with cleavage most pronounced following complete down-regulation of c-Myc.
Showed no PARP cleavage at lower concentrations where c-Myc levels were not fully reduced.
Chemical Information
-
CAS No. 2165322-95-0
-
Molecular Weight 420.23
-
Formula C17H17BrF3NO3
-
SMILES
FC(F)(F)OC1=CC=CC(=C1)COC2=CC=C(Br)C=C2CNCCO
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
USP25/28-IN-2 Related Classifications
HY-122686 Related Classifications
Powered by Bioz