Cat. No.: HY-N1050

Zederone

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Based on 1 Customer Validation

Zederone is a sesquiterpene. Zederone inhibits ovarian cancer cell proliferation through mTOR/p70s6K signalling pathway. Zederone inhibits CYP activities with IC₅₀s of 2.9 μM (CYP2B6), 9.2 μM (CYP2C9), 11,2 μM (CYP2C19) and >30 μM (CYP1A2 and CYP2D6). Zederone is hepatotoxic with LD₅₀ value at 24 hours in mice of approximately 223 mg/kg and cytotoxic against the KG1a cell line. Zederone shows antibacterial activity against a number of multi-drug resistant and Methicillin (HY-121544)-resistant Staphylococcus aureus strain. Zederone shows cognition improving capacity and assists in the modulation of gut bacterial dysbiosis.

For research use only. We do not sell to patients.

Purity: 99.61%
CAS No.: 7727-79-9
Formula: C₁₅H₁₈O₃
Molecular Weight:246.30

Storage:

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Zederone Chemical Structure

CAS No.: 7727-79-9

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target^[2]

mTOR

All mTOR Isoforms :

All Ribosomal S6 Kinase (RSK) Isoforms :

In Vitro

Zederone (0-80 μg/mL, 24 h) can inhibit the proliferation of human ovarian cancer cell line SKOV3 and arrest SKOV3 cells at G1 phase, and it may regulate the proliferation of SKOV3 cells through inhibiting mTOR/p70s6K signalling pathway^[2].
Zederone (0-30 μM, 24 h) significantly activates mouse PXR, significantly activates human PXR and CAR, and AhR shows no response in human hepatoma C3A cells^[3].
Zederone exhibits IC₅₀s in HLM incubations of 3.9 μM (CYP2B6), 9.9 μM (CYP3A4/M) and >50 μM (CYP1A2, CYP2C9, CYP2C19, CYP2D6 and CYP3A4/T)^[3].
Zederone shows antibacterial activity against SA1199B, ATCC25923, XU212, RN4220 and EMRSA15 with MICs of 128, 64, 128, 64 and 128 μg/ml^[4].
Zederone (0-100 μg/mL, 48 h) selectively kills leukemia stem cells (KG1a) with an IC₅₀ of 56.8 μg/mL and low cytotoxicity toward normal peripheral blood mononuclear cells (PBMCs) ^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[2]

Cell Line:

SKOV3 cells and IOSE80 cells
Concentration:

0, 5, 10, 20, 40 and 80 μg/mL
Incubation Time:

24 h
Result:

Inhibited the proliferation of SKOV3 cells in a dose-dependent manner but had no significant effect on the proliferation of IOSE80 cells.

Cell Cycle Analysis^[2]

Cell Line:

SKOV3 cells
Concentration:

10, 20, 40 μg/mL
Incubation Time:

24 h
Result:

Significantly increased the ratio of G1 phase cells, while the ratio of S phase cells and G2/M phase cells was significantly decreased.

Western Blot Analysis^[2]

Cell Line:

SKOV3 cells
Concentration:

10, 20, 40 μg/mL
Incubation Time:

24 h
Result:

Significantly decreased the expression levels of p-mTOR, p-p70s6K, mTOR and p70s6K.
Maintained the expression levels of p-PI3K and p-Akt.

In Vivo

Zederone (50-300 mg/kg, i.p., single dose) increases the expression of cytochrome P450 (Cyp) and leads to the formation of biological reactive metabolites and that cause the oxidative stress and liver cell injuries in mice^[1].
Zederone (10 mg/kg, p.o.) improves the fecal microbial profile in dementia induced rat model, showing a increased shift towards Lactobacillus genera from Bacteroides^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Acute liver toxicity model established in adult male ICR mice weighing 30 to 35 g (approximately 6-8 weeks of age)^[1]
Dosage:

50, 100, 150, 200, and 300 mg/kg
Administration:

Intraperitoneal injection (i.p.), single dose
Result:

Induced liver enlargement with scattered white foci over the organ.
Observed hepatic centrilobular necrosis with marked increases in plasma alanine transaminase activity and total bilirubin levels.
Decreased the activity of superoxide dismutase and the hepatic glutathione content at 200 mg/kg.
Increased the messenger RNA (mRNA) expression of Cyp2b10 and Cyp3a11 and proinflammatory cytokine tumor necrosis factor, and decreased the nuclear factor-E2-related factor 2 protein at 200 mg/kg.

Chemical Information

CAS No. 7727-79-9
Appearance Solid
Molecular Weight 246.30
Formula C₁₅H₁₈O₃
Color White to yellow
SMILES

CC1=COC(C/C(C)=C/CC[C@]2(C)[C@@]3([H])O2)=C1C3=O
Structure Classification
- Terpenoids
- Sesquiterpenes
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (203.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.0601 mL

20.3004 mL

40.6009 mL

5 mM

0.8120 mL

4.0601 mL

8.1202 mL

10 mM

0.4060 mL

2.0300 mL

4.0601 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

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Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (10.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (10.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (10.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (288 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Prapapan Pimkaew, et al. Zederone, a sesquiterpene from Curcuma elata Roxb, is hepatotoxic in mice. Int J Toxicol. 2013 Nov-Dec;32(6):454-62. [Content Brief]

[2]. Zhilei Zhang, et al. A study of zederone for the inhibition on ovarian cancer cell proliferation through mTOR/p70s6K signalling pathway. J BUON. 2020 Mar-Apr;25(2):785-791. [Content Brief]

[3]. Pimkaew P, et al. Interactions of sesquiterpenes zederone and germacrone with the human cytochrome P450 system. Toxicol In Vitro. 2013 Sep;27(6):2005-12. [Content Brief]

[4]. Kader M G, et al. Zederone from the rhizomes of Zingiber zerumbet and its anti-staphylococcal activity[J]. Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas, 2010, 9(1): 63-68.

[5]. Anuchapreeda S, et al. Characterization and biological properties of zederone and zedoarondiol from rhizomes of En-lueang (Curcuma cf. Amada)[J]. Natural Product Communications, 2018, 13(12).

[6]. Borah S, Sarkar P, Sharma HK. Zederone Improves the Fecal Microbial Profile in Dementia Induced Rat Model: A First Report. CNS Neurol Disord Drug Targets. 2022;21(4):335-342. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.0601 mL	20.3004 mL	40.6009 mL	101.5022 mL
	5 mM	0.8120 mL	4.0601 mL	8.1202 mL	20.3004 mL
	10 mM	0.4060 mL	2.0300 mL	4.0601 mL	10.1502 mL
	15 mM	0.2707 mL	1.3534 mL	2.7067 mL	6.7668 mL
	20 mM	0.2030 mL	1.0150 mL	2.0300 mL	5.0751 mL
	25 mM	0.1624 mL	0.8120 mL	1.6240 mL	4.0601 mL
	30 mM	0.1353 mL	0.6767 mL	1.3534 mL	3.3834 mL
	40 mM	0.1015 mL	0.5075 mL	1.0150 mL	2.5376 mL
	50 mM	0.0812 mL	0.4060 mL	0.8120 mL	2.0300 mL
	60 mM	0.0677 mL	0.3383 mL	0.6767 mL	1.6917 mL
	80 mM	0.0508 mL	0.2538 mL	0.5075 mL	1.2688 mL
	100 mM	0.0406 mL	0.2030 mL	0.4060 mL	1.0150 mL

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Zederone

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All mTOR Isoforms

All Ribosomal S6 Kinase (RSK) Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs