2. Anti-infection Apoptosis Autophagy
  3. Bacterial Apoptosis Autophagy
  4. 3-Nitropropanoic acid

3-Nitropropanoic acid  (Synonyms: β-Nitropropionic acid; Bovinocidin)

Cat. No.: HY-W012875 Purity: 99.93%
COA Handling Instructions

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3-Nitropropanoic acid (β-Nitropropionic acid) is an irreversible and orally active inhibitor of succinate dehydrogenase. 3-Nitropropanoic acid exhibits potent antimycobacterial activity with a MIC value of 3.3 μM. 3-Nitropropanoic acid can induce cell apoptosis.

For research use only. We do not sell to patients.

3-Nitropropanoic acid Chemical Structure

3-Nitropropanoic acid Chemical Structure

CAS No. : 504-88-1

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10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

3-Nitropropanoic acid Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

3-Nitropropanoic acid (β-Nitropropionic acid) is an irreversible and orally active inhibitor of succinate dehydrogenase. 3-Nitropropanoic acid exhibits potent antimycobacterial activity with a MIC value of 3.3 μM. 3-Nitropropanoic acid can induce cell apoptosis[1][2].

IC50 & Target

succinate dehydrogenase[1]
In Vitro

3-Nitropropanoic acid (5 mM, 3 h) induces autophagy and disrupts mitochondrial morphology in SH-SY5Y cells[3].
3-Nitropropanoic acid (5 mM, 24 h) induces oxidative stress and apoptosis of granulosa cells in geese[4].
3-Nitropropanoic acid (0-15 mM, 48 h) induces cell death in cultured rat hippocampal neurons[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

3-Nitropropanoic acid Related Antibodies

Western Blot Analysis[4]

Cell Line: Granulosa cells
Concentration: 5 mM
Incubation Time: 24 h
Result: Increaseed the levels of Bax, p53 and cleaved-Caspase 3 proteins, and the ratio of Bax/Bcl-2.
In Vivo

3-Nitropropanoic acid (20 mg/kg, i.p., BW/day for 4 days) induces oxidative stress, and increases lipid peroxidation in brain regions of the Wistar rat[6].
3-Nitropropanoic acid (100-200 mg/kg, i.p.) evokes seizures in mice[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

119.08

Formula

C3H5NO4
CAS No.
Appearance

Solid

Color

White to yellow

SMILES

O=C(O)CC[N+]([O-])=O
Structure Classification
Initial Source

fungi
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (1049.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 100 mg/mL (839.77 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 8.3977 mL 41.9886 mL 83.9772 mL
5 mM 1.6795 mL 8.3977 mL 16.7954 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.17 mg/mL (18.22 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (17.47 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.93%

COA (244 KB) HNMR (239 KB) RP-HPLC (250 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 8.3977 mL 41.9886 mL 83.9772 mL 209.9429 mL
5 mM 1.6795 mL 8.3977 mL 16.7954 mL 41.9886 mL
10 mM 0.8398 mL 4.1989 mL 8.3977 mL 20.9943 mL
15 mM 0.5598 mL 2.7992 mL 5.5985 mL 13.9962 mL
20 mM 0.4199 mL 2.0994 mL 4.1989 mL 10.4971 mL
25 mM 0.3359 mL 1.6795 mL 3.3591 mL 8.3977 mL
30 mM 0.2799 mL 1.3996 mL 2.7992 mL 6.9981 mL
40 mM 0.2099 mL 1.0497 mL 2.0994 mL 5.2486 mL
50 mM 0.1680 mL 0.8398 mL 1.6795 mL 4.1989 mL
60 mM 0.1400 mL 0.6998 mL 1.3996 mL 3.4990 mL
80 mM 0.1050 mL 0.5249 mL 1.0497 mL 2.6243 mL
100 mM 0.0840 mL 0.4199 mL 0.8398 mL 2.0994 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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3-Nitropropanoic acid Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

3-Nitropropanoic acid504-88-1β-Nitropropionic acid BovinocidinBacterialApoptosisAutophagySH-SY5YinfectionabtibacterialInhibitorinhibitorinhibit

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