Cat. No.: HY-N0058

4,5-Dicaffeoylquinic acid

(Synonyms: Isochlorogenic acid C; 4,5-diCQA)

12 Cited Publications

Customer Review

Based on 12 publication(s) in Google Scholar

4,5-Dicaffeoylquinic acid (Isochlorogenic acid C) is an antioxidant, can be isolated from Gynura divaricata and Laggera alata. 4,5-Dicaffeoylquinic acid reduces islet cell apoptosis and improves pancreatic function in type 2 diabetic mice, and has obvious inhibitory activities against yeast α-glucosidase. 4,5-Dicaffeoylquinic acid inhibits prostate cancer cells through cell cycle arrest. 4,5-Dicaffeoylquinic acid also has anti-apoptotic, anti-injury and anti-hepatitis B virus effects.

For research use only. We do not sell to patients.

Purity: 99.83%
CAS No.: 57378-72-0
Formula: C₂₅H₂₄O₁₂
Molecular Weight:516.45

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

4,5-Dicaffeoylquinic acid Chemical Structure

CAS No.: 57378-72-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Publications (12)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Endogenous Metabolite Isoforms :

In Vitro

4,5-Dicaffeoylquinic acid (0.1~100 μM; 72 h) has dose-dependent inhibitory activity against DU-145 prostate cancer cells, and induces cell cycle arrest, also induces inactivation of Bcl-2^[2].
4,5-Dicaffeoylquinic acid (1~100 μg/mL; 48 h) has anti-apoptotic effect in D-GalN-challenged HL-7702 hepatocytes, and improves significantly cell viability at concentrations of 10 to 100 μg/mL^[3].
4,5-Dicaffeoylquinic acid (1~100 μg/mL; 4 days) inhibits significantly expressions of HBsAg and HBeAg, and produces the maximum inhibition rates of 86.93 and 59.79% at 100 μg/mL on expressions of HBsAg and HBeAg, respectively^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[2]

Cell Line:

DU-145 cells
Concentration:

0.1~100 µM
Incubation Time:

72 h
Result:

Exhibits dose-dependent inhibitory activity against DU-145 prostate cancer cells with an IC₅₀ of 5 µM.

Cell Cycle Analysis^[2]

Cell Line:

DU-145 cells
Concentration:

5 µM
Incubation Time:

72 h
Result:

Decreased in numbers in G0/G1 phase and increased in S phase.

Apoptosis Analysis^[3]

Cell Line:

HL-7702 hepatocytes (exposure to 60 mM D-GalN for 6h)
Concentration:

1~100 μg/mL
Incubation Time:

48 h
Result:

Significantly reduced the caspase-3 and transforming growth factor β1 (TGFβ1) levels of the D-GalN-challenged hepatocytes at 10~100 µg/mL.

Cell Viability Assay^[3]

Cell Line:

HL-7702 hepatocytes (exposure to 80 mM D-GalN for 6h)
Concentration:

1~100 μg/mL
Incubation Time:

48 h
Result:

Improved significantly cell viability at concentrations of 10 to 100 µg/mL, and produced a maximum protection rate of 47.28% at 100 µg/mL.

In Vivo

4,5-Dicaffeoylquinic acid enhances the expression of GLUT2, GK, and PDX-1 protein, and effectively alleviate glycaemia and insulin resistance and enhance insulin sensitivity in type 2 diabetic mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 57378-72-0
Appearance Solid
Molecular Weight 516.45
Formula C₂₅H₂₄O₁₂
Color White to light yellow
SMILES

O=C([C@]1(O)C[C@@H](OC(/C=C/C2=CC=C(O)C(O)=C2)=O)[C@@H](OC(/C=C/C3=CC=C(O)C(O)=C3)=O)[C@H](O)C1)O
Synonyms

Isochlorogenic acid C; 4,5-diCQA
Structure Classification
- Phenols
- Polyphenols
- Ketones, Aldehydes, Acids
Initial Source
- Plants
- Compositae
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (12)

All WBIPCell Imaging/StainingIHCIn Vivo Efficacy Study

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Journal Impact Factor

Adv Sci (Weinh)

Dipeptidyl Peptidase 4 (DPP4) Exacerbates Osteoarthritis Progression in an Enzyme-Independent Manner. [Abstract]

2025 Feb;12(6):e2410525. PMID: 39680708

4,5-Dicaffeoylquinic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb;12(6):e2410525. [Abstract]

Different concentrations of 4,5-diCQA (4,5-Dicaffeoylquinic acid, 0-20 μM) were added to C28/I2 cells, and MYH9 levels were analyzed by western blot (n = 3 independent biological replicates).

4,5-Dicaffeoylquinic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb;12(6):e2410525. [Abstract]

Different concentrations of 4,5-diCQA (4,5-Dicaffeoylquinic acid, 0-20 μM) were added to DPP4-overexpressing C28/I2 cells, and MYH9 levels were analyzed by western blot (n = 3 independent biological replicates).

4,5-Dicaffeoylquinic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb;12(6):e2410525. [Abstract]

In C28/I2 cells transfected with MYH9-FLAG and DPP4‐HA plasmids, the addition of 4,5-diCQA (4,5-Dicaffeoylquinic acid), followed by IP pull-down of MYH9-FLAG and western blot analysis to detect DPP4‐HA levels (n = 3 independent biological replicates).

4,5-Dicaffeoylquinic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb;12(6):e2410525. [Abstract]

C28/I2 cells were treated with or without 4,5‐diCQA (4,5-Dicaffeoylquinic acid), and the interaction between DPP4 and MYH9 was analyzed using PLA.

4,5-Dicaffeoylquinic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb;12(6):e2410525. [Abstract]

IHC analysis of knee joints from Sham, DMM+PBS, and DMM+4,5-diCQA (4,5-Dicaffeoylquinic acid) groups.

4,5-Dicaffeoylquinic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb;12(6):e2410525. [Abstract]

Micro-CT analysis of knee joints from Sham, DMM+PBS, and DMM+4,5-diCQA (4,5-Dicaffeoylquinic acid) groups, including quantitative analysis of osteophyte number and SBP thickness.

4,5-Dicaffeoylquinic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb;12(6):e2410525. [Abstract]

Schematic diagram of the experimental design: 10-week-old mice underwent Sham or DMM surgery. One week after surgery, the DMM group received weekly intra-articular injections of PBS or 4,5-diCQA (4,5-Dicaffeoylquinic acid) until 8 weeks after surgery, when knee joints were collected. Schematic diagram of the experimental design: 18-month-old mice received intra-articular injections of AAV2-NC, AAV2-Dpp4-WT, or AAV2-Dpp4-MUT. Two weeks later, AAV2-NC mice received weekly PBS injections, while AAV2-Dpp4-WT and AAV2-Dpp4-MUT mice received weekly 4,5-diCQA injections. Knee joints were collected 3 months later.
Phytomedicine

A novel mechanism of Reduning injection in sepsis treatment: Targeting inflammatory kinases TBK1 and IKKβ. [Abstract]

2026 Feb:151:157776. PMID: 41512388
Int J Biol Macromol

Structure-based discovery of dual-target inhibitors of the helicase from bagaza virus. [Abstract]

2025 Mar:294:139536. PMID: 39765299
J Agric Food Chem

Raw and Sous-Vide-Cooked Red Cardoon Stalks ( Cynara cardunculus L. var. altilis DC): (Poly)phenol Bioaccessibility, Anti-inflammatory Activity in the Gastrointestinal Tract, and Prebiotic Activity. [Abstract]

2021 Aug 18;69(32):9270-9286. PMID: 34347467
J Agric Food Chem

Isolation, Identification, and Synthesis of a New Prenylated Cinnamic Acid Derivative from Brazilian Green Propolis and Simultaneous Quantification of Bioactive Components by LC-MS/MS. [Abstract]

2019 Nov 6;67(44):12303-12312. PMID: 31597041
Cell Biosci

Coffee as a dietary strategy to prevent SARS-CoV-2 infection. [Abstract]

2023 Nov 14;13(1):210. PMID: 37964389
Foods

Effects of Cooking Methods on Caffeoylquinic Acids and Radical Scavenging Activity of Sweet Potato. [Abstract]

2024 Apr 3;13(7):1101. PMID: 38611405
ACS Omega

Selective Enhancement of Caffeoylquinic Acid Derivative via UV Irradiation and Validation of Analytical Method in the Aerial Aster × chusanensis Y. S. Lim. [Abstract]

2025 Sep 22;10(38):44260-44269. PMID: 41048756
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
Biologia

(2019) 74:1569-1577.
SSRN

2026 Apr 8.
Chemistry of Plant Raw Material

2025.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (193.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.9363 mL

9.6815 mL

19.3630 mL

5 mM

0.3873 mL

1.9363 mL

3.8726 mL

10 mM

0.1936 mL

0.9681 mL

1.9363 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.84 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (4.84 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (4.84 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (283 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Yin XL, et al. Gynura divaricata rich in 3, 5-/4, 5-dicaffeoylquinic acid and chlorogenic acid reduces islet cell apoptosis and improves pancreatic function in type 2 diabetic mice. Nutr Metab (Lond). 2018 Oct 10;15:73. [Content Brief]

[2]. Lodise O, et al. Inhibition of Prostate Cancer Cells by 4,5-Dicaffeoylquinic Acid through Cell Cycle Arrest. Prostate Cancer. 2019 May 23;2019:4520645. [Content Brief]

[3]. Shaoqing Hu, et al.Evaluation of anti-apoptotic, anti-injury and antihepatitis B virus effects of isochlorogenic acid C in vitro. Journal of Medicinal Plants Research Vol. 6(16), pp. 3199-3206 30 April, 2012

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.9363 mL	9.6815 mL	19.3630 mL	48.4074 mL
	5 mM	0.3873 mL	1.9363 mL	3.8726 mL	9.6815 mL
	10 mM	0.1936 mL	0.9681 mL	1.9363 mL	4.8407 mL
	15 mM	0.1291 mL	0.6454 mL	1.2909 mL	3.2272 mL
	20 mM	0.0968 mL	0.4841 mL	0.9681 mL	2.4204 mL
	25 mM	0.0775 mL	0.3873 mL	0.7745 mL	1.9363 mL
	30 mM	0.0645 mL	0.3227 mL	0.6454 mL	1.6136 mL
	40 mM	0.0484 mL	0.2420 mL	0.4841 mL	1.2102 mL
	50 mM	0.0387 mL	0.1936 mL	0.3873 mL	0.9681 mL
	60 mM	0.0323 mL	0.1614 mL	0.3227 mL	0.8068 mL
	80 mM	0.0242 mL	0.1210 mL	0.2420 mL	0.6051 mL
	100 mM	0.0194 mL	0.0968 mL	0.1936 mL	0.4841 mL

4,5-Dicaffeoylquinic acid

12 Cited Publications

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4,5-Dicaffeoylquinic acid

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Publications Citing Use of MedChemExpress (MCE) 4,5-Dicaffeoylquinic acid

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (12)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

4,5-Dicaffeoylquinic acid Related Classifications

HY-N0058 Related Classifications