Bemesetron (Synonyms: MDL 72222)

Cat. No.: HY-B1541 Purity: ≥99.0%: FAQs
Data Sheet SDS COA Handling Instructions

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Bemesetron (MDL 72222) is a selective 5-HT₃ receptor antagonist with an IC₅₀ of 0.33 nM. Neuroprotective effect.

For research use only. We do not sell to patients.

Bemesetron Chemical Structure

CAS No. : 40796-97-2

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5 mg	USD 75	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
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Description

Bemesetron (MDL 72222) is a selective 5-HT₃ receptor antagonist with an IC₅₀ of 0.33 nM^[1]. Neuroprotective effect^[2].

In Vitro

Blockade of 5-HT₃ receptor with Bemesetron (MDL7222) reduces hydrogen peroxide-induced neurotoxicity in cultured rat cortical cells. Bemesetron (0.01, 0.1 and 1 μM, 15 hours) and Y25130 (0.05, 0.5 and 5 μM) concentration-dependently reduce the H₂O₂-induced decrease of MTT reduction showing 74.9±2.4 and 79.0 ±2.5% with 1 μM and 5 μM, respectively, which are maximal effects^[2].
Pretreatment (20 minutes) with Bemesetron (1 μM), Y25130 (5 μM) or MK-801 (10 μM) significantly, but not completely, inhibits the H₂O₂-induced elevation of [Ca²⁺]_c^[2].
Bemesetron (1 μM, 15 hours) significantly blocks the H₂O₂-induced increase of caspase-3 immunoreactivity^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	Primary cortical neuronal cells
Concentration:	0.01-1 μM
Incubation Time:	20 minutes (pretreatment); 15 hours (post-incubation)
Result:	Concentration-dependently reduced the H₂O₂-induced decrease of MTT reduction showing 74.9±2.4% with 1 μM, which was maximal effect.

Western Blot Analysis^[2]

Cell Line:	Primary cortical neuronal cells
Concentration:	1 μM
Incubation Time:	15 hours
Result:	Blocked significantly the H₂O₂-induced increase of caspase-3 immunoreactivity.

In Vivo

Bemesetron (0.1, 1 and 10 mg/kg; i.p.) is used in male adult albino mice. The lowest dose do not cause any significant change in catalepsy. However, Bemesetron (1 mg/kg) causes a significant reduction of catalepsy (from 90 min after Haloperidol), while 10 mg/kg significantly potentiates the phenomenon (from 60 min after Haloperidol). The maximum inhibition of catalepsy (about 75%) occurs at 120 min, and the maximum potentiation (about 4.5-times the control value) occurs at 60 min after Haloperidol^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male adult albino mice, weighing 26-36 g^[3]
Dosage:	0.1-10 mg/kg
Administration:	Intraperitoneally injected, 20 min (pretreatment) +180 min (treatment)
Result:	Reduced catalepsy significantly at a dose of 1 mg/kg, whilst 10 mg/kg potentiated the phenomenon and 0.1 mg/kg was found to be without effect.

Molecular Weight

314.21

Formula

C₁₅H₁₇Cl₂NO₂

CAS No.

40796-97-2

Appearance

Solid

Color

White to off-white

SMILES

O=C(O[C@@H]1C[C@@H](N2C)CC[C@@H]2C1)C3=CC(Cl)=CC(Cl)=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 2 mg/mL (6.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1826 mL	15.9129 mL	31.8258 mL
5 mM	0.6365 mL	3.1826 mL	6.3652 mL
10 mM	---	---	---

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: ≥99.0%

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Data Sheet (250 KB) SDS (0 KB)

COA (250 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.1826 mL	15.9129 mL	31.8258 mL	79.5646 mL
DMSO	5 mM	0.6365 mL	3.1826 mL	6.3652 mL	15.9129 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Bemesetron40796-97-2MDL 72222MDL72222MDL-72222Inhibitorinhibitorinhibit

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