Betamipron (Synonyms: N-Benzoyl-β-alanine)

Name: Betamipron
Brand: MedChemExpress
SKU: HY-B1127
Rating: 4.5 (1 reviews)

Cat. No.: HY-B1127 Purity: 98.69%: Data Sheet
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Betamipron (N-benzoyl-β-alanine) is a carbapenem β-lactam antibiotic with antibacterial activity against most Gram-positive and Gram-negative aerobic and anaerobic bacteria. Betamipron can target and inhibit renal organic anion transporters, alleviate renal injury caused by Cisplatin (HY-17394), without interfering with the biological activity of cisplatin. Betamipron is often used in combination with panipenem, which can attenuate the renal effects induced by panipenem and slightly promote the proliferation of Clostridium difficile in the cecum. Betamipron can be used in studies related to renal injury and bacterial infection.

For research use only. We do not sell to patients.

Betamipron Chemical Structure

CAS No. : 3440-28-6

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
50 g	Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of Betamipron:

Betamipron (Standard) Get quote

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target^[1]

β-lactam

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	6 μM Compound: Betamipron	TP_TRANSPORTER: inhibition of Adefovir uptake in OAT1-expressing CHO cells TP_TRANSPORTER: inhibition of Adefovir uptake in OAT1-expressing CHO cells	[PMID: 10991954]
S2	IC₅₀	39 μM Compound: Betamipron	TP_TRANSPORTER: inhibition of PHA uptake (PHA: 5uM) in hOAT1-expressing S2 cells TP_TRANSPORTER: inhibition of PHA uptake (PHA: 5uM) in hOAT1-expressing S2 cells	[PMID: 14978359]
S2	IC₅₀	47.5 μM Compound: Betamipron	TP_TRANSPORTER: inhibition of ES uptake (ES: 50nM) in rOAT3-expressing S2 cells TP_TRANSPORTER: inhibition of ES uptake (ES: 50nM) in rOAT3-expressing S2 cells	[PMID: 14978359]
S2	IC₅₀	483 μM Compound: Betamipron	TP_TRANSPORTER: inhibition of ES uptake (ES: 50nM) in hOAT4-expressing S2 cells TP_TRANSPORTER: inhibition of ES uptake (ES: 50nM) in hOAT4-expressing S2 cells	[PMID: 14978359]
S2	IC₅₀	9.92 μM Compound: Betamipron	TP_TRANSPORTER: inhibition of ES uptake (ES: 50nM) in hOAT3-expressing S2 cells TP_TRANSPORTER: inhibition of ES uptake (ES: 50nM) in hOAT3-expressing S2 cells	[PMID: 14978359]

In Vitro

Betamipron (1:1 ratio with panipenem) inhibits renal dehydropeptidase-I and kidney anion transport to reduce panipenem-associated kidney toxicity and prevent panipenem hydrolysis by renal DHP-I^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Betamipron (100 mg/kg; s.c.; once daily; 5 days) as part of a 1:1 panipenem/betamipron formulation results in Clostridium difficile overgrowth in mouse cecal contents by 7 days post-dosing^[1].
Betamipron (250 mg/kg; i.p.; single dose) significantly suppresses Cisplatin (HY-17394)-induced nephrotoxicity in male Wistar rats; significantly reduced Cisplatin-induced weight loss in male ddY mice and decreased serum BUN levels; and did not alter the antileukemic efficacy of cisplatin in male BDF1 mice carrying P388 leukemia^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	ICI strain (male, 5 weeks old, 20g)^[1]
Dosage:	100 mg/kg (as part of a 1:1 panipenem/betamipron formulation)
Administration:	s.c.; once daily; 5 days
Result:	Reduced Clostridium difficile counts in cecal contents to below 10² CFU/g in all 5 mice 1 day after the end of dosing. Resulted in Clostridium difficile counts of 10² CFU/g in 1 mouse and 10³ CFU/g in 4 mice 7 days after the end of dosing.

Animal Model:	Wistar (male, 235-365 g, cisplatin-induced nephrotoxicity model) ddY (male, 25-32 g, cisplatin-induced nephrotoxicity model) BDF1 (male, 5 weeks old, 21-24.5 g, P388 leukemia model) ^[2]
Dosage:	250 mg/kg
Administration:	i.p.; single dose (1 hour after cisplatin)
Result:	Blocked cisplatin-induced weight loss, with a 9.37% weight gain versus a 10.8% loss in the cisplatin group. Studies were conducted on male Wistar rats with cisplatin-induced nephrotoxicity. Decreased serum BUN and creatinine to normal levels, which remained high in rats given cisplatin alone. Studies were conducted on male Wistar rats with cisplatin-induced nephrotoxicity. Recovered cisplatin-altered water intake, urine volume and pH. Studies were conducted on male Wistar rats with cisplatin-induced nephrotoxicity. Normalized abnormal urinary biochemical indicators caused by cisplatin. Studies were conducted on male Wistar rats with cisplatin-induced nephrotoxicity. Mitigated cisplatin-triggered weight loss obviously compared with the combination of cisplatin and alkaline solution. Studies were conducted on male ddY mice with cisplatin-induced nephrotoxicity. Reduced elevated serum BUN in mice receiving cisplatin plus alkaline solution. Studies were conducted on male ddY mice with cisplatin-induced nephrotoxicity. Achieved similar survival benefits as cisplatin combined with alkaline solution. Studies were conducted on male BDF1 mice with P388 leukemia. Extended survival in all cisplatin-treated mice compared with controls. Studies were conducted on male BDF1 mice with P388 leukemia.

Molecular Weight

193.20

Formula

C₁₀H₁₁NO₃

CAS No.

3440-28-6

Appearance

Solid

Color

White to off-white

SMILES

O=C(O)CCNC(C1=CC=CC=C1)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (517.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.1760 mL	25.8799 mL	51.7598 mL
5 mM	1.0352 mL	5.1760 mL	10.3520 mL
10 mM	0.5176 mL	2.5880 mL	5.1760 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (12.94 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (12.94 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (12.94 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.69%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (244 KB) LCMS (105 KB)

Handling Instructions (2659 KB)

References

[1]. Watanabe K, et al. In vitro antibacterial activity of a new carbapenem, panipenem, against anaerobic bacteria[J]. Chemotherapy, 1991, 39(Suppl 3): 46-54.

[2]. Tokunaga J, et al. Betamipron reduces cisplatin nephrotoxicity in rodents without modifying its antileukemic activity in mice. Ren Fail. 1997;19(3):425-438.

[3]. El-Gamal MI, et al. Recent updates of carbapenem antibiotics. Eur J Med Chem. 2017;131:185-195.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.1760 mL	25.8799 mL	51.7598 mL	129.3996 mL
	5 mM	1.0352 mL	5.1760 mL	10.3520 mL	25.8799 mL
	10 mM	0.5176 mL	2.5880 mL	5.1760 mL	12.9400 mL
	15 mM	0.3451 mL	1.7253 mL	3.4507 mL	8.6266 mL
	20 mM	0.2588 mL	1.2940 mL	2.5880 mL	6.4700 mL
	25 mM	0.2070 mL	1.0352 mL	2.0704 mL	5.1760 mL
	30 mM	0.1725 mL	0.8627 mL	1.7253 mL	4.3133 mL
	40 mM	0.1294 mL	0.6470 mL	1.2940 mL	3.2350 mL
	50 mM	0.1035 mL	0.5176 mL	1.0352 mL	2.5880 mL
	60 mM	0.0863 mL	0.4313 mL	0.8627 mL	2.1567 mL
	80 mM	0.0647 mL	0.3235 mL	0.6470 mL	1.6175 mL
	100 mM	0.0518 mL	0.2588 mL	0.5176 mL	1.2940 mL

Betamipron Related Classifications

Infection Endocrinology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Betamipron3440-28-6N-Benzoyl-β-alanineAntibioticBacterialdehydropeptidase-Imale Wistar ratscisplatin-induced nephrotoxicityClostridium difficileP388 leukemiapanipenemkidney anion transportmale BDF1 micemale ddY miceInhibitorinhibitorinhibit

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Betamipron (Synonyms: N-Benzoyl-β-alanine)

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Complete Stock Solution Preparation Table

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