Chebulagic acid

Name: Chebulagic acid
Brand: MedChemExpress
SKU: HY-N1996
Rating: 5.0 (5 reviews)

Cat. No.: HY-N1996 Purity: 99.66%: Data Sheet
COA

SDS
Handling Instructions
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Chebulagic acid is a COX-LOX dual inhibitor isolated from the fruits of Terminalia chebula Retz, on angiogenesis. Chebulagic acid is a M2 serine to asparagine 31 mutation (S31N) inhibitor and influenza antiviral. Chebulagic acid also against SARS-CoV-2 viral replication with an EC₅₀ of 9.76 μM.

For research use only. We do not sell to patients.

CAS No. : 23094-71-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
2 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of Chebulagic acid:

Chebulagic acid (Standard) Get quote

5 Publications Citing Use of MCE Chebulagic acid

ELISA

RT-PCR

Cell Proliferation/Viability Assay

: Chebulagic acid purchased from MedChemExpress. Usage Cited in: Pharmaceutics. 2024 Jun 2;16(6):751.; A549 cells were pre-treated with active compounds (Gallic acid, Chebulagic acid, and Chebulinic acid) (0–20 µg/mL) for 24 h. Then, the cells were exposed to CoV2-SP (100 ng/mL) for 3 h. The IL-6 (A), IL-1β (B), and IL-18 secretions (C) in the culture supernatant were examined by ELISA.

: Chebulagic acid purchased from MedChemExpress. Usage Cited in: Pharmaceutics. 2024 Jun 2;16(6):751.; Inhibitory effects of active compounds (Gallic acid, Chebulagic acid and Chebulinic acid) on the IL-6 (A), IL-1β (B), IL-18 (C), and NLRP3 gene expressions (D) in CoV2-SP-induced A549 cells. A549 cells were pre-treated with active compounds (gallic acid, chebulagic acid and chebulinic acid) (0–20 µg/mL) for 24 h. Then, the cells were exposed to CoV2-SP (100 ng/mL) for 3 h. The mRNA expressions were determined using RT-qPCR.

: Chebulagic acid purchased from MedChemExpress. Usage Cited in: Pharmaceutics. 2024 Jun 2;16(6):751.; The effects of Chebulagic acid and chebulinic acid inhibited the NLRP3 inflammasome pathway in CoV2-SP-induced A549 cells. A549 cells were pre-treated with chebulagic acid and Chebulinic acid (0–20 µg/mL) for 24 h. Then, the cells were exposed to CoV2-SP (100 ng/mL) for 3 h. The inhibitory effects of chebulagic acid and chebulinic acid on the expression of NLRP3, ASC, and pro-caspase-1 (p50) and cleaved-caspase-1 (p20) proteins in A549 cells are displayed in western blot.

: Chebulagic acid purchased from MedChemExpress. Usage Cited in: Pharmaceutics. 2024 Jun 2;16(6):751.; Chebulagic acid (0-50 μg/mL; 24, 48 h) showed no cytotoxicity towards A549 cells.

: Chebulagic acid purchased from MedChemExpress. Usage Cited in: Antiviral Res. 2021 Jun:190:105075. [Abstract]; Chebulagic acid (CHLA; 3.7, 11.1, 33.3 μM; 48 h) inhibited the plaque formation of SARS-CoV-2 in a dose-dependent manner (Vero-E6 monolayers grown in 12-well plates were pre-treated with increasing concentrations of test compound for 1 h, followed by infection with SARS-CoV-2 (MOI of 0.0001) in the presence of test compounds).

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5-LOX 12-LOX 15-LOX

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Chebulagic acid can enhance the autophagy. Chebulagic acid exert anti-inflammatory and anti-infective effects. Chebulagic acid also shows a protective effect against 1-methyl-4-phenylpyridinium (MPP+)-induce cytotoxicity which mimics the pathological symptom of Parkinson's disease. Chebulagic acid inhibits the LPS-induced upregulation of TNF-α and IL-1β in a dose- and time-dependent manner. Furthermore, LPS-activated MAPK signaling is inhibited by Chebulagic acid treatment in the EA.hy926 cells.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

954.66

Formula

C₄₁H₃₀O₂₇

CAS No.

23094-71-5

Appearance

Solid

Color

White to light yellow

SMILES

OC(CC(C(O[C@H]([C@@H](COC(C1=CC(O)=C2O)=O)O[C@@H]3OC(C4=CC(O)=C(O)C(O)=C4)=O)[C@H](OC(C5=CC(O)=C(O)C(O)=C5C1=C2O)=O)[C@H]3OC6=O)=O)C(C7O)C(C6=CC(O)=C8O)=C8OC7=O)=O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (104.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.0475 mL	5.2375 mL	10.4749 mL
5 mM	0.2095 mL	1.0475 mL	2.0950 mL
10 mM	0.1047 mL	0.5237 mL	1.0475 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.83 mg/mL (0.87 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.83 mg/mL (0.87 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 0.83 mg/mL (0.87 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.85%

Select Batch:

Data Sheet (284 KB) SDS (252 KB)

COA (265 KB) HNMR (153 KB) LCMS (107 KB)

Handling Instructions (2659 KB)

References

[1]. Kim HJ et al. Neuroprotective Effect of Chebulagic Acid via Autophagy Induction in SH-SY5Y Cells. Biomol Ther (Seoul). 2014 Jul;22(4):275-81. [Content Brief]

[2]. Liu Y et al. Chebulagic acid inhibits the LPS-induced expression of TNF-α and IL-1β in endothelial cells by suppressing MAPK activation. Exp Ther Med. 2015 Jul;10(1):263-268. [Content Brief]

[3]. Athira AP et al. Inhibition of Angiogenesis In Vitro by Chebulagic Acid: A COX-LOX Dual Inhibitor. Int J Vasc Med. 2013;2013:843897. [Content Brief]

[4]. Maggie C. Duncan, et al. Virtual Screening Identifies Chebulagic Acid as an Inhibitor of the M2(S31N) Viral Ion Channel and Influenza A Virus. Molecules 2020, 25, 2903.

[5]. RuikunDu, et al. Discovery of Chebulagic Acid and Punicalagin as Novel Allosteric Inhibitors of SARS-CoV-2 3CLpro. Antivir Res. 2021, 105075.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.0475 mL	5.2375 mL	10.4749 mL	26.1873 mL
	5 mM	0.2095 mL	1.0475 mL	2.0950 mL	5.2375 mL
	10 mM	0.1047 mL	0.5237 mL	1.0475 mL	2.6187 mL
	15 mM	0.0698 mL	0.3492 mL	0.6983 mL	1.7458 mL
	20 mM	0.0524 mL	0.2619 mL	0.5237 mL	1.3094 mL
	25 mM	0.0419 mL	0.2095 mL	0.4190 mL	1.0475 mL
	30 mM	0.0349 mL	0.1746 mL	0.3492 mL	0.8729 mL
	40 mM	0.0262 mL	0.1309 mL	0.2619 mL	0.6547 mL
	50 mM	0.0209 mL	0.1047 mL	0.2095 mL	0.5237 mL
	60 mM	0.0175 mL	0.0873 mL	0.1746 mL	0.4365 mL
	80 mM	0.0131 mL	0.0655 mL	0.1309 mL	0.3273 mL
	100 mM	0.0105 mL	0.0524 mL	0.1047 mL	0.2619 mL

Chebulagic acid Related Classifications

Inflammation/Immunology Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Chebulagic acid23094-71-5COXLipoxygenaseSARS-CoVInfluenza VirusCyclooxygenaseLOXSARS coronavirusInhibitorinhibitorinhibit

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Chebulagic acid

Customer Review

Other Forms of Chebulagic acid:

Chebulagic acid Related Antibodies

5 Publications Citing Use of MCE Chebulagic acid

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All COX Isoform Specific Products:

View All Lipoxygenase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Chebulagic acid Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Chebulagic acid Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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