Cytidine (Synonyms: Cytosine β-D-riboside; Cytosine-1-β-D-ribofuranoside)

Name: Cytidine
Brand: MedChemExpress
SKU: HY-B0158
Rating: 5.0 (8 reviews)

Cat. No.: HY-B0158 Purity: 99.97%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Cytidine is a pyrimidine nucleoside and acts as a component of RNA. Cytidine is a precursor of uridine. Cytidine controls neuronal-glial glutamate cycling, affecting cerebral phospholipid metabolism, catecholamine synthesis, and mitochondrial function.

For research use only. We do not sell to patients.

CAS No. : 65-46-3

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	Get quote
50 g	Get quote

or Bulk Inquiry

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 8 publication(s) in Google Scholar

Other Forms of Cytidine:

8 Publications Citing Use of MCE Cytidine

RT-PCR

Cell Imaging/Staining

Cell Proliferation/Viability Assay

Cell Migration/Invasion Assay

: Cytidine purchased from MedChemExpress. Usage Cited in: Imeta. 2026 Feb 24.; Cytidine (15 μM; 12 h) significantly increased the transcription of the viral gene 2A in EV-D68-infected RD cells.

: Cytidine purchased from MedChemExpress. Usage Cited in: Information Processing in Agriculture. 2026 Mar 3.; Cytidine (200 μM; 24 h) significantly suppressed CYP19A1 expression in female gonadal cells.

: Cytidine purchased from MedChemExpress. Usage Cited in: J Pharm Biomed Anal. 2025 Jun 17:265:117026. [Abstract]; Cytidine (50-100 µM; 72 h) inhibited the proliferation of both EC (KYSE150 cell) and GC (HGC27 cell) cell lines.

: Cytidine purchased from MedChemExpress. Usage Cited in: J Pharm Biomed Anal. 2025 Jun 17:265:117026. [Abstract]; Cytidine (50-100 µM; 72 h) inhibited the migration of both EC (KYSE150 cell) and GC (HGC27 cell) cell lines.

: Cytidine purchased from MedChemExpress. Usage Cited in: University of Hawaii. 2023 May.; Cytidine (205-410 µM; 4 d) significantly mitigated the negative effects of NHC on EBs.

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Human Endogenous Metabolite Microbial Metabolite

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
HOS-TE85	IC₅₀	2.6 μg/mL Compound: Cytidine	Inhibitory effect tested in vitro for the growth of Osteosarcoma MNNG-HOS cell line Inhibitory effect tested in vitro for the growth of Osteosarcoma MNNG-HOS cell line	[PMID: 1895307]
KHOS-321H	IC₅₀	0.27 μg/mL Compound: Cytidine	Inhibitory effect tested in vitro for the growth of Osteosarcoma KHOS-321H cell line Inhibitory effect tested in vitro for the growth of Osteosarcoma KHOS-321H cell line	[PMID: 1895307]
MKN-28	IC₅₀	4.5 μg/mL Compound: Cytidine	Inhibitory effect tested in vitro for the growth of Stomach adenocarcinoma MKN- 28 cell line Inhibitory effect tested in vitro for the growth of Stomach adenocarcinoma MKN- 28 cell line	[PMID: 1895307]
NUGC-4	IC₅₀	> 100 μg/mL Compound: Cytidine	Inhibitory effect tested in vitro for the growth of Stomach adenocarcinoma NUGC-4 cell line Inhibitory effect tested in vitro for the growth of Stomach adenocarcinoma NUGC-4 cell line	[PMID: 1895307]
OST	IC₅₀	> 100 μg/mL Compound: Cytidine	Inhibitory effect tested in vitro for the growth of Osteosarcoma OST cell line Inhibitory effect tested in vitro for the growth of Osteosarcoma OST cell line	[PMID: 1895307]
PC-13	IC₅₀	> 100 μg/mL Compound: Cytidine	Inhibitory effect tested in vitro for the growth of Lung adenocarcinoma PC-13 cell line Inhibitory effect tested in vitro for the growth of Lung adenocarcinoma PC-13 cell line	[PMID: 1895307]
PC-3	IC₅₀	> 100 μg/mL Compound: Cytidine	Inhibitory effect tested in vitro for the growth of Lung adenocarcinoma PC-3 cell line Inhibitory effect tested in vitro for the growth of Lung adenocarcinoma PC-3 cell line	[PMID: 1895307]
PC-8	IC₅₀	0.28 μg/mL Compound: Cytidine	Inhibitory effect tested in vitro for the growth of Lung adenocarcinoma PC-8 cell line Inhibitory effect tested in vitro for the growth of Lung adenocarcinoma PC-8 cell line	[PMID: 1895307]
PC-9	IC₅₀	1.6 μg/mL Compound: Cytidine	Inhibitory effect tested in vitro for the growth of Lung adenocarcinoma PC-9 cell line Inhibitory effect tested in vitro for the growth of Lung adenocarcinoma PC-9 cell line	[PMID: 1895307]
QG-56	IC₅₀	> 100 μg/mL Compound: Cytidine	Inhibitory effect tested in vitro for the growth of Lung squamous-cell carcinoma QG-56 cell line Inhibitory effect tested in vitro for the growth of Lung squamous-cell carcinoma QG-56 cell line	[PMID: 1895307]
SK-ES1	IC₅₀	0.09 μg/mL Compound: Cytidine	Inhibitory effect tested in vitro for the growth of Osteosarcoma SK-ES-1 cell line Inhibitory effect tested in vitro for the growth of Osteosarcoma SK-ES-1 cell line	[PMID: 1895307]
ST-KM-1	IC₅₀	> 100 μg/mL Compound: Cytidine	Inhibitory effect tested in vitro for the growth of Stomach adenocarcinoma ST-KM cell line Inhibitory effect tested in vitro for the growth of Stomach adenocarcinoma ST-KM cell line	[PMID: 1895307]
SW480	IC₅₀	> 100 μg/mL Compound: Cytidine	Inhibitory effect tested in vitro for the growth of colon adenocarcinoma SW-480 cell line Inhibitory effect tested in vitro for the growth of colon adenocarcinoma SW-480 cell line	[PMID: 1895307]

In Vivo

Cytidine decreases glutamate/glutamine levels and induces earlier improvement of depressive symptoms^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

243.22

Formula

C₉H₁₃N₃O₅

CAS No.

65-46-3

Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1[C@@H](O)[C@H](N2C(N=C(N)C=C2)=O)O[C@@H]1CO

Structure Classification

Others

Initial Source

Animals

Microorganisms

Widespread

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

H₂O : ≥ 50 mg/mL (205.58 mM)

DMSO : 50 mg/mL (205.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.1115 mL	20.5575 mL	41.1150 mL
5 mM	0.8223 mL	4.1115 mL	8.2230 mL
10 mM	0.4112 mL	2.0558 mL	4.1115 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (10.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (411.15 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.97%

Select Batch:

Data Sheet (277 KB) SDS (394 KB)

COA (253 KB) RP-HPLC (205 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Jonas DA, et al. Safety considerations of DNA in food. Ann Nutr Metab. 2001;45(6):235-54. [Content Brief]

[2]. Wurtman RJ, et al. Effect of oral CDP-choline on plasma choline and uridine levels in humans. Biochem Pharmacol. 2000 Oct 1;60(7):989-92. [Content Brief]

[3]. Machado-Vieira R, et, al. New therapeutic targets for mood disorders. ScientificWorldJournal. 2010 Apr 13;10:713-26. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	4.1115 mL	20.5575 mL	41.1150 mL	102.7876 mL
	5 mM	0.8223 mL	4.1115 mL	8.2230 mL	20.5575 mL
	10 mM	0.4112 mL	2.0558 mL	4.1115 mL	10.2788 mL
	15 mM	0.2741 mL	1.3705 mL	2.7410 mL	6.8525 mL
	20 mM	0.2056 mL	1.0279 mL	2.0558 mL	5.1394 mL
	25 mM	0.1645 mL	0.8223 mL	1.6446 mL	4.1115 mL
	30 mM	0.1371 mL	0.6853 mL	1.3705 mL	3.4263 mL
	40 mM	0.1028 mL	0.5139 mL	1.0279 mL	2.5697 mL
	50 mM	0.0822 mL	0.4112 mL	0.8223 mL	2.0558 mL
	60 mM	0.0685 mL	0.3426 mL	0.6853 mL	1.7131 mL
	80 mM	0.0514 mL	0.2570 mL	0.5139 mL	1.2848 mL
	100 mM	0.0411 mL	0.2056 mL	0.4112 mL	1.0279 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Cytidine Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cytidine65-46-3Cytosine β-D-riboside Cytosine-1-β-D-ribofuranosideNucleoside Antimetabolite/AnalogEndogenous MetaboliteuridineRNAnucleosideribofuranoseInhibitorinhibitorinhibit

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Cytidine (Synonyms: Cytosine β-D-riboside; Cytosine-1-β-D-ribofuranoside)

Customer Review

Other Forms of Cytidine:

Cytidine Related Antibodies

8 Publications Citing Use of MCE Cytidine

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Cytidine Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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