2. Anti-infection Metabolic Enzyme/Protease
  3. Influenza Virus Endogenous Metabolite
  4. N6-Methyladenosine

N6-Methyladenosine  (Synonyms: 6-Methyladenosine; N-Methyladenosine)

Cat. No.: HY-N0086 Purity: 99.75%
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N6-Methyladenosine is the most prevalent internal (non-cap) modification present in the messenger RNA (mRNA) of all higher eukaryotes. N6-Methyladenosine can modifies viral RNAs and has antiviral activities.

For research use only. We do not sell to patients.

CAS No. : 1867-73-8

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10 mM * 1 mL in DMSO
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Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of N6-Methyladenosine:

N6-Methyladenosine Related Antibodies

Top Publications Citing Use of Products

    N6-Methyladenosine purchased from MedChemExpress. Usage Cited in: Mol Ther. 2024 Dec 4;32(12):4418-4434.  [Abstract]

    IFNb mRNA and protein levels in the N6-Methyladenosine (m6A)-pretreated MEF cells. Cells were treated with vehicle, 5 μM UZH1a or 50 μM m6A nucleoside for 12 h, then infected with 1 MOI Adv for 6 h and 12 h.

    N6-Methyladenosine purchased from MedChemExpress. Usage Cited in: Mol Ther. 2024 Dec 4;32(12):4418-4434.  [Abstract]

    N6-Methyladenosine (m6A, 50 μM, 12 h). Representative fluorescent images of MEF cells transfected with 200 ng/mL DNA for 30 min. Green: fluorescein 5-isothiocyanate (FITC)-labeled DNA; blue: 4′,6-diamidino-2-phenylindole (DAPI).

    N6-Methyladenosine purchased from MedChemExpress. Usage Cited in: Mol Ther. 2024 Dec 4;32(12):4418-4434.  [Abstract]

    N6-Methyladenosine (m6A, 50 μM, 12 h). Representative fluorescent images of cGAS-DNA condensates in the m6A-pretreated MEF cells transfected with 200 ng/mL fluorescein 5-isothiocyanate (FITC)-labeled DNA for 30 min.

    N6-Methyladenosine purchased from MedChemExpress. Usage Cited in: Mol Ther. 2024 Dec 4;32(12):4418-4434.  [Abstract]

    (Left) Representative images of 10 μM YF2 incubated with 5 μM 45-bp DNA and/or 5 μM 45-bp RNA with or without N6-Methyladenosine (m6A) modification for 10 min. (Right) Turbidity assays of serial dilutions of YF2 incubated with 1 μM DNA and/or RNA for 10 min. Drilled water was used as Mock. n = 3.

    N6-Methyladenosine purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Mar 27;14(1):1694.  [Abstract]

    N6-Methyladenosine (m6A) methylation dot blotting shows decreased m6A methylation in LFS astrocytes. Dot blotting is performed to identify polyadenylated mRNAs immunoblotted with anti-m6A antibodies (upper panel). Methylene blue staining of total mRNA is used as a loading control (lower panel).

    N6-Methyladenosine purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Mar 27;14(1):1694.  [Abstract]

    N6-Methyladenosine (m6A) methylation dot blotting indicates increased m6A methylation upon depletion of mutant p53 in LFS astrocytes. Dot blotting identifies polyadenylated mRNA isolated from shCtrl and shp53 transduced LFS astrocytes and immunoblotted with anti-m6A antibodies (upper panel). Methylene blue staining of total mRNA is used as a loading control (lower panel).

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    Human Endogenous Metabolite Microbial Metabolite

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    N6-Methyladenosine is the most prevalent internal (non-cap) modification present in the messenger RNA (mRNA) of all higher eukaryotes. N6-Methyladenosine can modifies viral RNAs and has antiviral activities.

    IC50 & Target

    Microbial Metabolite

     

    Human Endogenous Metabolite

     

    Cellular Effect
    Cell Line Type Value Description References
    HT-29 IC50
    > 100 μM
    Compound: N6-Me-Ado
    In vitro inhibitory activity against human colon carcinoma HT-29 cell line
    In vitro inhibitory activity against human colon carcinoma HT-29 cell line
    		[PMID: 16033277]
    K562 IC50
    > 100 μM
    Compound: N6-Me-Ado
    In vitro inhibitory activity against human leukemia K562IU cell line
    In vitro inhibitory activity against human leukemia K562IU cell line
    		[PMID: 16033277]
    K562 IC50
    > 100 μM
    Compound: N6-Me-Ado
    In vitro inhibitory activity against human myelogenous leukemia K562 cell line
    In vitro inhibitory activity against human myelogenous leukemia K562 cell line
    		[PMID: 16033277]
    MCF7 IC50
    > 100 μM
    Compound: N6-Me-Ado
    In vitro inhibitory activity against human breast carcinoma MCF-7 cell line
    In vitro inhibitory activity against human breast carcinoma MCF-7 cell line
    		[PMID: 16033277]
    In Vitro

    N6-methyladenosine (m6A) is selectively recognized by the human YTH domain family 2 (YTHDF2) protein to regulate mRNA degradation. N6-methyladenosine (m6A), a prevalent internal modification in the messenger RNA of all eukaryotes, is post-transcriptionally installed by m6A methyltransferase (e.g., MT-A70) within the consensus sequence of G(m6A)C (70%) or A(m6A)C (30%). N6-methyladenosine (m6A)-containing RNAs are greatly enriched in the YTHDF-bound portion and diminished in the flow-through portion[1]. N6-methyladenosine (m6A), the most abundant internal RNA modification, functions in diverse biological processes, including regulation of embryonic stem cell self-renewal and differentiation. N6-methyladenosine (m6A) is a large protein complex, consisting in part of methyltransferase-like 3 (METTL3) and methyltransferase-like 14 (METTL14) catalytic subunits[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    N6-Methyladenosine Related Antibodies
    Molecular Weight

    281.27

    Formula

    C11H15N5O4
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N2C3=C(N=C2)C(NC)=NC=N3
    Structure Classification
    Initial Source

    Eukarya
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 31 mg/mL (110.21 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 5.56 mg/mL (19.77 mM; ultrasonic and warming and heat to 60°C)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.5553 mL 17.7765 mL 35.5530 mL
    5 mM 0.7111 mL 3.5553 mL 7.1106 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (7.40 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (7.40 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.84%

    SDS (393 KB)

    COA (253 KB) HNMR (135 KB) RP-HPLC (234 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 3.5553 mL 17.7765 mL 35.5530 mL 88.8826 mL
    5 mM 0.7111 mL 3.5553 mL 7.1106 mL 17.7765 mL
    10 mM 0.3555 mL 1.7777 mL 3.5553 mL 8.8883 mL
    15 mM 0.2370 mL 1.1851 mL 2.3702 mL 5.9255 mL
    DMSO 20 mM 0.1778 mL 0.8888 mL 1.7777 mL 4.4441 mL
    25 mM 0.1422 mL 0.7111 mL 1.4221 mL 3.5553 mL
    30 mM 0.1185 mL 0.5926 mL 1.1851 mL 2.9628 mL
    40 mM 0.0889 mL 0.4444 mL 0.8888 mL 2.2221 mL
    50 mM 0.0711 mL 0.3555 mL 0.7111 mL 1.7777 mL
    60 mM 0.0593 mL 0.2963 mL 0.5926 mL 1.4814 mL
    80 mM 0.0444 mL 0.2222 mL 0.4444 mL 1.1110 mL
    100 mM 0.0356 mL 0.1778 mL 0.3555 mL 0.8888 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    N6-Methyladenosine Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    N6-Methyladenosine1867-73-86-Methyladenosine N-MethyladenosineInfluenza VirusEndogenous MetaboliteInhibitorinhibitorinhibit

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