Cat. No.: HY-N0377

Liquiritigenin

(Synonyms: 4',7-Dihydroxyflavanone)

14 Cited Publications

Customer Review

Based on 14 publication(s) in Google Scholar

Liquiritigenin, a flavanone isolated from Glycyrrhiza uralensis, is a highly selective estrogen receptor β (ERβ) agonist with an EC₅₀ of 36.5 nM for activation of the ERE tk-Luc.

For research use only. We do not sell to patients.

Purity: 99.59%
CAS No.: 578-86-9
Formula: C₁₅H₁₂O₄
Molecular Weight:256.25

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Liquiritigenin Chemical Structure

CAS No.: 578-86-9

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (14)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

Liquiritigenin, a flavanone isolated from Glycyrrhiza uralensis, is a highly selective estrogen receptor β (ERβ) agonist with an EC₅₀ of 36.5 nM for activation of the ERE tk-Luc.

IC₅₀ & Target

EC50: 36.5 nM (activation of the ERE tk-Luc)^[1]

All Estrogen Receptor/ERR Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>100 μM Compound: 2	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 18440233]
B16	IC₅₀	5 μg/mL Compound: 6, liquiritigenin	Cytotoxicity against mouse B16 cells after 72 hrs by MTT assay Cytotoxicity against mouse B16 cells after 72 hrs by MTT assay	[PMID: 15787450]
B16-BL6	IC₅₀	97.7 μM Compound: 2	Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay	[PMID: 18440233]
CCRF-CEM	IC₅₀	>25 μg/mL Compound: 6, liquiritigenin	Cytotoxicity against human CEM cells after 72 hrs by MTT assay Cytotoxicity against human CEM cells after 72 hrs by MTT assay	[PMID: 15787450]
DU-145	IC₅₀	>100 μM Compound: 7	Cytotoxicity against human DU145 cells after 72 hrs by MTT assay Cytotoxicity against human DU145 cells after 72 hrs by MTT assay	[PMID: 20494492]
HCT-116	IC₅₀	37.2 μM Compound: 7	Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay	[PMID: 20494492]
HCT-8	IC₅₀	19.5 μg/mL Compound: 6, liquiritigenin	Cytotoxicity against human HCT8 cells after 72 hrs by MTT assay Cytotoxicity against human HCT8 cells after 72 hrs by MTT assay	[PMID: 15787450]
HeLa	IC₅₀	>100 μM Compound: 2	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay Cytotoxicity against human HeLa cells after 72 hrs by MTT assay	[PMID: 18440233]
HeLa	IC₅₀	>100 μM Compound: 7	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay Cytotoxicity against human HeLa cells after 72 hrs by MTT assay	[PMID: 20494492]
HL-60	IC₅₀	22.5 μg/mL Compound: 6, liquiritigenin	Cytotoxicity against human HL60 cells after 72 hrs by MTT assay Cytotoxicity against human HL60 cells after 72 hrs by MTT assay	[PMID: 15787450]
HT-1080	IC₅₀	>100 μM Compound: 2	Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay	[PMID: 18440233]
K562	IC₅₀	>100 μM Compound: 7	Cytotoxicity against human K562 cells after 72 hrs by MTT assay Cytotoxicity against human K562 cells after 72 hrs by MTT assay	[PMID: 20494492]
Lewis lung carcinoma cell line	IC₅₀	>100 μM Compound: 2	Cytotoxicity against mouse LLC cells after 72 hrs by MTT assay Cytotoxicity against mouse LLC cells after 72 hrs by MTT assay	[PMID: 18440233]
MCF7	IC₅₀	>25 μg/mL Compound: 6, liquiritigenin	Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 15787450]
MDCK	CC₅₀	301 μM Compound: 10	Cytotoxicity against MDCK cells after 72 hrs Cytotoxicity against MDCK cells after 72 hrs	[PMID: 24313801]
Peritoneal macrophage	IC₅₀	85 μM Compound: kp24	Inhibition of LPS-stimulated nitric oxide production in ddy mouse peritoneal macrophages measured after 20 hrs by Greiss method Inhibition of LPS-stimulated nitric oxide production in ddy mouse peritoneal macrophages measured after 20 hrs by Greiss method	[PMID: 27955927]
RAW264.7	IC₅₀	16.34 μM Compound: 69	Anti-inflammatory activity against mouse RAW264.7 cells assessed as reduction in NO production pre-incubated with compound for 1 hrs followed by LPS-stimulation and measured for 1 hrs by Western blot analysis Anti-inflammatory activity against mouse RAW264.7 cells assessed as reduction in NO production pre-incubated with compound for 1 hrs followed by LPS-stimulation and measured for 1 hrs by Western blot analysis	[PMID: 37683361]
SGC-7901	IC₅₀	92.4 μM Compound: 7	Cytotoxicity against human SGC7901 cells after 72 hrs by MTT assay Cytotoxicity against human SGC7901 cells after 72 hrs by MTT assay	[PMID: 20494492]

In Vitro

Liquiritigenin produces a dose-response activation of ERE tk-Luc in the U2OS cells transfected with ERβ, but not ERα. Liquiritigenin produces a dose-dependent activation and a time-dependent increase of the CECR6, NKG2E and NKD with ERβ but not with ERα. The ERβ-selectivity of liquiritigenin is due to the selective recruitment of the coactivator steroid receptor coactivator-2 to target genes. Liquiritigenin exhibits similar binding affinities for ERα and ERβ, and causes the recruitment of SRC-2 to target genes selectively in ERβ cells^[1]. Pretreatment of MC3T3-E1 cells with liquiritigenin prevents the MG-induced cell death and production of protein adduct, intracellular reactive oxygen species, mitochondrial superoxide, cardiolipin peroxidation, and TNF-α in osteoblastic MC3T3-E1 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

In a mouse xenograph model, liquiritigenin does not stimulate uterine size or tumorigenesis of MCF-7 breast cancer cells^[1]. Treatment with liquiritigenin significantly reduces the concentrations of pro-inflammatory cytokines including interleukin (IL)-6, IL-1β and tumor necrosis factor (TNF)-α in serum and hippocampus^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 578-86-9
Appearance Solid
Molecular Weight 256.25
Formula C₁₅H₁₂O₄
Color White to light yellow
SMILES

O=C1C[C@@H](C2=CC=C(O)C=C2)OC3=CC(O)=CC=C13
Synonyms

4',7-Dihydroxyflavanone
Structure Classification
- Flavonoids
- Flavonones
- Phenols
- Polyphenols
Initial Source
- Microorganisms
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (14)

All Cell Proliferation/Viability AssayFlow CytometryELISACell Imaging/StainingBio/Physico-chemical AssayWBIHCHistological Imaging/Staining

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Journal Impact Factor

Acta Pharm Sin B

Chrysin serves as a novel inhibitor of DGK α/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC). [Abstract]

2021 Jan;11(1):143-155. PMID: 33532186
Food Chem

Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]

2025 Dec 30:497:146992. PMID: 41285060
Food Chem

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]

2025 May 31:489:144992. PMID: 40466530

Liquiritigenin purchased from MedChemExpress. Usage Cited in: Food Chem. 2025 May 31:489:144992. [Abstract]

Viability of platelets treated with gradient concentrations of liquiritigenin (5, 10, 20, 40, 80, 160, 320 μM, 6 h) as determined by MTT assay; n = 3.

Liquiritigenin purchased from MedChemExpress. Usage Cited in: Food Chem. 2025 May 31:489:144992. [Abstract]

Flow cytometric analysis of CD61⁺CD62P⁺ expression in ADP-stimulated platelets following liquiritigenin (20, 40, or 80 μM) treatment; n = 4.

Liquiritigenin purchased from MedChemExpress. Usage Cited in: Food Chem. 2025 May 31:489:144992. [Abstract]

Levels of PF4, TXA2, sCD40L, and β-TG in platelet supernatants after ADP stimulation, measured by ELISA in the presence or absence of liquiritigenin (20, 40, or 80 μM); n = 3.

Liquiritigenin purchased from MedChemExpress. Usage Cited in: Food Chem. 2025 May 31:489:144992. [Abstract]

Representative three-dimensional reconstructions of iFluor™ 555-labeled platelets (red) adherent to fibrinogen-coated surfaces in the presence or absence of Liquiritigenin (20, 40, or 80 μM). Transmission electron micrographs of ADP-activated platelets showing dense granule morphology before and after liquiritigenin treatment. White arrows indicate dense granules; red arrows indicate granule secretion. Scale bar = 1 μm; magnification: × 8000.

Liquiritigenin purchased from MedChemExpress. Usage Cited in: Food Chem. 2025 May 31:489:144992. [Abstract]

Quantitative analysis of platelet adhesion thickness on fibrinogen-coated surfaces following ADP activation with or without Liquiritigenin (20, 40, or 80 μM) treatment; n = 3.

Liquiritigenin purchased from MedChemExpress. Usage Cited in: Food Chem. 2025 May 31:489:144992. [Abstract]

Representative fluorescence images showing interactions between P-selectin–labeled platelets and PECAM-1–labeled HUVECs in the presence or absence of Liquiritigenin (20, 40, or 80 μM). Scale bar = 50 μm; magnification: × 400.

Liquiritigenin purchased from MedChemExpress. Usage Cited in: Food Chem. 2025 May 31:489:144992. [Abstract]

Western blot analysis of Syk phosphorylation mediated by GPVI activation with CRP as an agonist, in the presence or absence of Liquiritigenin (20, 40, or 80 μM); n = 3.

Liquiritigenin purchased from MedChemExpress. Usage Cited in: Food Chem. 2025 May 31:489:144992. [Abstract]

Liquiritigenin (10, 20, and 40 mg/kg, oral gavage, once daily for five consecutive days). Flow cytometric analysis of peripheral blood platelet activation in the APE model with or without liquiritigenin treatment. Total platelets were labeled with APC anti-CD61; activated platelets were labeled with FITC anti-CD62P.

Liquiritigenin purchased from MedChemExpress. Usage Cited in: Food Chem. 2025 May 31:489:144992. [Abstract]

Liquiritigenin (10, 20, and 40 mg/kg, oral gavage, once daily for five consecutive days). Representative images of lung tissue sections stained with H&E, and immunofluorescence staining of thrombotic areas. Thrombotic lesions are indicated by red arrows; activated platelets were labeled with P-selectin. Scale bar = 100 μm; magnification: × 200.

Liquiritigenin purchased from MedChemExpress. Usage Cited in: Food Chem. 2025 May 31:489:144992. [Abstract]

Liquiritigenin (10, 20, and 40 mg/kg, oral gavage, once daily for five consecutive days). Histopathological scoring of lung tissues from APE mice with or without liquiritigenin treatment; n = 3.

Liquiritigenin purchased from MedChemExpress. Usage Cited in: Food Chem. 2025 May 31:489:144992. [Abstract]

Liquiritigenin (10, 20, and 40 mg/kg, oral gavage, once daily for five consecutive days). Representative tissue immunofluorescence images showing integrin αIIbβ3 activation sites (platelets) and CD31 endothelial cells in pulmonary microvessels, with corresponding immunohistochemical detection of CD40L expression. scale bar = 50 μm; magnification: × 400.
Phytomedicine

Liquiritigenin attenuates thrombosis by inhibiting P2Y12R-mediated Src/PLCγ2 signaling and cytoplasmic Ca2+ mobilization in platelets. [Abstract]

2025 Dec:149:157553. PMID: 41273866
Phytomedicine

Amoebicidal action of isoliquiritigenin and glabridin from Glycyrrhiza species: Mechanisms and effects against Acanthamoeba castellanii. [Abstract]

2025 Sep:145:157081. PMID: 40701128
Am J Chin Med

Liquiritigenin, an Active Ingredient of Liquorice, Alleviates Acute Kidney Injury by VKORC1-Mediated Ferroptosis Inhibition. [Abstract]

2024 Aug 28:1-20. PMID: 39192677
J Ethnopharmacol

Liquiritigenin alleviates inflammation and mitochondrial dysfunction in acute pancreatitis via ERβ-VDAC1 signaling. [Abstract]

2025 Sep 3:120526. PMID: 40912483
Nutrients

Interpreting the Mechanism of Active Ingredients in Polygonati Rhizoma in Treating Depression by Combining Systemic Pharmacology and In Vitro Experiments. [Abstract]

2024 Apr 14;16(8):1167. PMID: 38674858
J Pharm Biomed Anal

Chemical profiling of Zhi-Ke-Bao pills and its potential mechanism against cough by ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry and network pharmacology. [Abstract]

2024 Sep 1:247:116257. PMID: 38815520
Arch Biochem Biophys

Liquiritigenin inhibits the migration, invasion, and EMT of prostate cancer through activating ER stress. [Abstract]

2024 Nov:761:110184. PMID: 39447623
Reprod Sci

Liquiritigenin Restores Declined Oocyte Quality and Improves Embryo Development in Aged Mice. [Abstract]

2025 Aug 27. PMID: 40866779
Cancer Genet

Mechanistic study of Liquiritigenin inhibiting bladder cancer cell proliferation and migration by regulating STING1. [Abstract]

2025 Jun:294-295:156-170. PMID: 40347832
bioRxiv

2025 Feb 19.
SSRN

2024 Mar 21.

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (487.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.9024 mL

19.5122 mL

39.0244 mL

5 mM

0.7805 mL

3.9024 mL

7.8049 mL

10 mM

0.3902 mL

1.9512 mL

3.9024 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.42 mg/mL (9.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.42 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (24.2 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.42 mg/mL (9.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.42 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (24.2 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.42 mg/mL (9.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.42 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (24.2 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocol

Kinase Assay^[1]

The relative binding affinity of liquiritigenin to pure full-length ERα and ERβ is determined using ERα and ERβ competitor assay kits. Fluorescence polarization of the fluorophore-tagged estrogen bound to ERα and ERβ in the presence of increasing amounts of competitor ligand or extract is determined using the GENios Pro microplate reader with fluorescein excitation (485 nM) and emission (530 nM) filters^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[1]

Mice: MCF-7 (250,000) cells are grafted under the kidney capsule of nude mice. Five mice per group are treated with a continuous infusion using osmotic pumps containing vehicle, E2 (0.4 mg) or liquiritigenin (2 mg) that infused 2.5 μL/h for 1 month. After one month of treatment, the tumors and uteri are removed and analyzed^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Mersereau JE, et al. Liquiritigenin is a plant-derived highly selective estrogen receptor beta agonist. Mol Cell Endocrinol. 2008 Feb 13;283(1-2):49-57. [Content Brief]

[2]. Suh KS, et al. Protective effect of liquiritigenin against methylglyoxal cytotoxicity in osteoblastic MC3T3-E1 cells. Food Funct. 2014 Jul 25;5(7):1432-40. [Content Brief]

[3]. Tao W, et al. Liquiritigenin reverses depression-like behavior in unpredictable chronic mild stress-induced mice by regulating PI3K/Akt/mTOR mediated BDNF/TrkB pathway. Behav Brain Res. 2016 Jul 15;308:177-86. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.9024 mL	19.5122 mL	39.0244 mL	97.5610 mL
	5 mM	0.7805 mL	3.9024 mL	7.8049 mL	19.5122 mL
	10 mM	0.3902 mL	1.9512 mL	3.9024 mL	9.7561 mL
	15 mM	0.2602 mL	1.3008 mL	2.6016 mL	6.5041 mL
	20 mM	0.1951 mL	0.9756 mL	1.9512 mL	4.8780 mL
	25 mM	0.1561 mL	0.7805 mL	1.5610 mL	3.9024 mL
	30 mM	0.1301 mL	0.6504 mL	1.3008 mL	3.2520 mL
	40 mM	0.0976 mL	0.4878 mL	0.9756 mL	2.4390 mL
	50 mM	0.0780 mL	0.3902 mL	0.7805 mL	1.9512 mL
	60 mM	0.0650 mL	0.3252 mL	0.6504 mL	1.6260 mL
	80 mM	0.0488 mL	0.2439 mL	0.4878 mL	1.2195 mL
	100 mM	0.0390 mL	0.1951 mL	0.3902 mL	0.9756 mL

Keywords

Liquiritigenin

14 Cited Publications

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Liquiritigenin

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Publications Citing Use of MedChemExpress (MCE) Liquiritigenin

All Estrogen Receptor/ERR Isoforms

Biological Activity

Chemical Information

Publications (14)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Liquiritigenin Related Classifications

Cat. No.: HY-N0377