2. Vitamin D Related/Nuclear Receptor
  3. Estrogen Receptor/ERR
  4. Liquiritigenin

Liquiritigenin  (Synonyms: 4',7-Dihydroxyflavanone)

Cat. No.: HY-N0377 Purity: 99.59%
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Liquiritigenin, a flavanone isolated from Glycyrrhiza uralensis, is a highly selective estrogen receptor β (ERβ) agonist with an EC50 of 36.5 nM for activation of the ERE tk-Luc.

For research use only. We do not sell to patients.

CAS No. : 578-86-9

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Customer Review

Based on 14 publication(s) in Google Scholar

Other Forms of Liquiritigenin:

Liquiritigenin Related Antibodies

Top Publications Citing Use of Products

    Liquiritigenin purchased from MedChemExpress. Usage Cited in: Food Chem. 2025 May 31:489:144992.  [Abstract]

    Viability of platelets treated with gradient concentrations of liquiritigenin (5, 10, 20, 40, 80, 160, 320 μM, 6 h) as determined by MTT assay; n = 3.

    Liquiritigenin purchased from MedChemExpress. Usage Cited in: Food Chem. 2025 May 31:489:144992.  [Abstract]

    Flow cytometric analysis of CD61⁺CD62P⁺ expression in ADP-stimulated platelets following liquiritigenin (20, 40, or 80 μM) treatment; n = 4.

    Liquiritigenin purchased from MedChemExpress. Usage Cited in: Food Chem. 2025 May 31:489:144992.  [Abstract]

    Levels of PF4, TXA2, sCD40L, and β-TG in platelet supernatants after ADP stimulation, measured by ELISA in the presence or absence of liquiritigenin (20, 40, or 80 μM); n = 3.

    Liquiritigenin purchased from MedChemExpress. Usage Cited in: Food Chem. 2025 May 31:489:144992.  [Abstract]

    Representative three-dimensional reconstructions of iFluor™ 555-labeled platelets (red) adherent to fibrinogen-coated surfaces in the presence or absence of Liquiritigenin (20, 40, or 80 μM). Transmission electron micrographs of ADP-activated platelets showing dense granule morphology before and after liquiritigenin treatment. White arrows indicate dense granules; red arrows indicate granule secretion. Scale bar = 1 μm; magnification: × 8000.

    Liquiritigenin purchased from MedChemExpress. Usage Cited in: Food Chem. 2025 May 31:489:144992.  [Abstract]

    Quantitative analysis of platelet adhesion thickness on fibrinogen-coated surfaces following ADP activation with or without Liquiritigenin (20, 40, or 80 μM) treatment; n = 3.

    Liquiritigenin purchased from MedChemExpress. Usage Cited in: Food Chem. 2025 May 31:489:144992.  [Abstract]

    Representative fluorescence images showing interactions between P-selectin–labeled platelets and PECAM-1–labeled HUVECs in the presence or absence of Liquiritigenin (20, 40, or 80 μM). Scale bar = 50 μm; magnification: × 400.

    Liquiritigenin purchased from MedChemExpress. Usage Cited in: Food Chem. 2025 May 31:489:144992.  [Abstract]

    Western blot analysis of Syk phosphorylation mediated by GPVI activation with CRP as an agonist, in the presence or absence of Liquiritigenin (20, 40, or 80 μM); n = 3.

    Liquiritigenin purchased from MedChemExpress. Usage Cited in: Food Chem. 2025 May 31:489:144992.  [Abstract]

    Liquiritigenin (10, 20, and 40 mg/kg, oral gavage, once daily for five consecutive days). Flow cytometric analysis of peripheral blood platelet activation in the APE model with or without liquiritigenin treatment. Total platelets were labeled with APC anti-CD61; activated platelets were labeled with FITC anti-CD62P.

    Liquiritigenin purchased from MedChemExpress. Usage Cited in: Food Chem. 2025 May 31:489:144992.  [Abstract]

    Liquiritigenin (10, 20, and 40 mg/kg, oral gavage, once daily for five consecutive days). Representative images of lung tissue sections stained with H&E, and immunofluorescence staining of thrombotic areas. Thrombotic lesions are indicated by red arrows; activated platelets were labeled with P-selectin. Scale bar = 100 μm; magnification: × 200.

    Liquiritigenin purchased from MedChemExpress. Usage Cited in: Food Chem. 2025 May 31:489:144992.  [Abstract]

    Liquiritigenin (10, 20, and 40 mg/kg, oral gavage, once daily for five consecutive days). Histopathological scoring of lung tissues from APE mice with or without liquiritigenin treatment; n = 3.

    Liquiritigenin purchased from MedChemExpress. Usage Cited in: Food Chem. 2025 May 31:489:144992.  [Abstract]

    Liquiritigenin (10, 20, and 40 mg/kg, oral gavage, once daily for five consecutive days). Representative tissue immunofluorescence images showing integrin αIIbβ3 activation sites (platelets) and CD31 endothelial cells in pulmonary microvessels, with corresponding immunohistochemical detection of CD40L expression. scale bar = 50 μm; magnification: × 400.

    View All Estrogen Receptor/ERR Isoform Specific Products:

    View All Isoforms
    Estrogen receptor ERα ERβ ERRα ERRβ ERRγ

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Liquiritigenin, a flavanone isolated from Glycyrrhiza uralensis, is a highly selective estrogen receptor β (ERβ) agonist with an EC50 of 36.5 nM for activation of the ERE tk-Luc.

    IC50 & Target

    EC50: 36.5 nM (activation of the ERE tk-Luc)[1]
    Cellular Effect
    Cell Line Type Value Description References
    A549 IC50
    > 100 μM
    Compound: 2
    Cytotoxicity against human A549 cells after 72 hrs by MTT assay
    Cytotoxicity against human A549 cells after 72 hrs by MTT assay
    		[PMID: 18440233]
    B16 IC50
    5 μg/mL
    Compound: 6, liquiritigenin
    Cytotoxicity against mouse B16 cells after 72 hrs by MTT assay
    Cytotoxicity against mouse B16 cells after 72 hrs by MTT assay
    		[PMID: 15787450]
    B16-BL6 IC50
    97.7 μM
    Compound: 2
    Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay
    Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay
    		[PMID: 18440233]
    CCRF-CEM IC50
    > 25 μg/mL
    Compound: 6, liquiritigenin
    Cytotoxicity against human CEM cells after 72 hrs by MTT assay
    Cytotoxicity against human CEM cells after 72 hrs by MTT assay
    		[PMID: 15787450]
    DU-145 IC50
    > 100 μM
    Compound: 7
    Cytotoxicity against human DU145 cells after 72 hrs by MTT assay
    Cytotoxicity against human DU145 cells after 72 hrs by MTT assay
    		[PMID: 20494492]
    HCT-116 IC50
    37.2 μM
    Compound: 7
    Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay
    Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay
    		[PMID: 20494492]
    HCT-8 IC50
    19.5 μg/mL
    Compound: 6, liquiritigenin
    Cytotoxicity against human HCT8 cells after 72 hrs by MTT assay
    Cytotoxicity against human HCT8 cells after 72 hrs by MTT assay
    		[PMID: 15787450]
    HL-60 IC50
    22.5 μg/mL
    Compound: 6, liquiritigenin
    Cytotoxicity against human HL60 cells after 72 hrs by MTT assay
    Cytotoxicity against human HL60 cells after 72 hrs by MTT assay
    		[PMID: 15787450]
    HT-1080 IC50
    > 100 μM
    Compound: 2
    Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay
    Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay
    		[PMID: 18440233]
    HeLa IC50
    > 100 μM
    Compound: 2
    Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
    Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
    		[PMID: 18440233]
    HeLa IC50
    > 100 μM
    Compound: 7
    Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
    Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
    		[PMID: 20494492]
    K562 IC50
    > 100 μM
    Compound: 7
    Cytotoxicity against human K562 cells after 72 hrs by MTT assay
    Cytotoxicity against human K562 cells after 72 hrs by MTT assay
    		[PMID: 20494492]
    MCF7 IC50
    > 25 μg/mL
    Compound: 6, liquiritigenin
    Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay
    Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay
    		[PMID: 15787450]
    MDCK CC50
    301 μM
    Compound: 10
    Cytotoxicity against MDCK cells after 72 hrs
    Cytotoxicity against MDCK cells after 72 hrs
    		[PMID: 24313801]
    Peritoneal macrophage IC50
    85 μM
    Compound: kp24
    Inhibition of LPS-stimulated nitric oxide production in ddy mouse peritoneal macrophages measured after 20 hrs by Greiss method
    Inhibition of LPS-stimulated nitric oxide production in ddy mouse peritoneal macrophages measured after 20 hrs by Greiss method
    		[PMID: 27955927]
    RAW264.7 IC50
    16.34 μM
    Compound: 69
    Anti-inflammatory activity against mouse RAW264.7 cells assessed as reduction in NO production pre-incubated with compound for 1 hrs followed by LPS-stimulation and measured for 1 hrs by Western blot analysis
    Anti-inflammatory activity against mouse RAW264.7 cells assessed as reduction in NO production pre-incubated with compound for 1 hrs followed by LPS-stimulation and measured for 1 hrs by Western blot analysis
    		[PMID: 37683361]
    SGC-7901 IC50
    92.4 μM
    Compound: 7
    Cytotoxicity against human SGC7901 cells after 72 hrs by MTT assay
    Cytotoxicity against human SGC7901 cells after 72 hrs by MTT assay
    		[PMID: 20494492]
    In Vitro

    Liquiritigenin produces a dose-response activation of ERE tk-Luc in the U2OS cells transfected with ERβ, but not ERα. Liquiritigenin produces a dose-dependent activation and a time-dependent increase of the CECR6, NKG2E and NKD with ERβ but not with ERα. The ERβ-selectivity of liquiritigenin is due to the selective recruitment of the coactivator steroid receptor coactivator-2 to target genes. Liquiritigenin exhibits similar binding affinities for ERα and ERβ, and causes the recruitment of SRC-2 to target genes selectively in ERβ cells[1]. Pretreatment of MC3T3-E1 cells with liquiritigenin prevents the MG-induced cell death and production of protein adduct, intracellular reactive oxygen species, mitochondrial superoxide, cardiolipin peroxidation, and TNF-α in osteoblastic MC3T3-E1 cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Liquiritigenin Related Antibodies
    In Vivo

    In a mouse xenograph model, liquiritigenin does not stimulate uterine size or tumorigenesis of MCF-7 breast cancer cells[1]. Treatment with liquiritigenin significantly reduces the concentrations of pro-inflammatory cytokines including interleukin (IL)-6, IL-1β and tumor necrosis factor (TNF)-α in serum and hippocampus[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    256.25

    Formula

    C15H12O4
    CAS No.
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    O=C1C[C@@H](C2=CC=C(O)C=C2)OC3=CC(O)=CC=C13
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 125 mg/mL (487.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.9024 mL 19.5122 mL 39.0244 mL
    5 mM 0.7805 mL 3.9024 mL 7.8049 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.42 mg/mL (9.44 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.42 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (24.2 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.42 mg/mL (9.44 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.42 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (24.2 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.59%

    SDS (396 KB)

    COA (246 KB) HNMR (182 KB) RP-HPLC (248 KB) MS (71 KB)

    Handling Instructions (2659 KB)
    References
    Kinase Assay
    [1]

    The relative binding affinity of liquiritigenin to pure full-length ERα and ERβ is determined using ERα and ERβ competitor assay kits. Fluorescence polarization of the fluorophore-tagged estrogen bound to ERα and ERβ in the presence of increasing amounts of competitor ligand or extract is determined using the GENios Pro microplate reader with fluorescein excitation (485 nM) and emission (530 nM) filters[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [1]

    Mice: MCF-7 (250,000) cells are grafted under the kidney capsule of nude mice. Five mice per group are treated with a continuous infusion using osmotic pumps containing vehicle, E2 (0.4 mg) or liquiritigenin (2 mg) that infused 2.5 μL/h for 1 month. After one month of treatment, the tumors and uteri are removed and analyzed[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.9024 mL 19.5122 mL 39.0244 mL 97.5610 mL
    5 mM 0.7805 mL 3.9024 mL 7.8049 mL 19.5122 mL
    10 mM 0.3902 mL 1.9512 mL 3.9024 mL 9.7561 mL
    15 mM 0.2602 mL 1.3008 mL 2.6016 mL 6.5041 mL
    20 mM 0.1951 mL 0.9756 mL 1.9512 mL 4.8780 mL
    25 mM 0.1561 mL 0.7805 mL 1.5610 mL 3.9024 mL
    30 mM 0.1301 mL 0.6504 mL 1.3008 mL 3.2520 mL
    40 mM 0.0976 mL 0.4878 mL 0.9756 mL 2.4390 mL
    50 mM 0.0780 mL 0.3902 mL 0.7805 mL 1.9512 mL
    60 mM 0.0650 mL 0.3252 mL 0.6504 mL 1.6260 mL
    80 mM 0.0488 mL 0.2439 mL 0.4878 mL 1.2195 mL
    100 mM 0.0390 mL 0.1951 mL 0.3902 mL 0.9756 mL
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    Liquiritigenin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Liquiritigenin578-86-94',7-DihydroxyflavanoneEstrogen Receptor/ERRInhibitorinhibitorinhibit

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