1. Metabolic Enzyme/Protease
    Autophagy
  2. Endogenous Metabolite
    Cytochrome P450
    Autophagy
  3. Metyrapone

Metyrapone (Synonyms: Su-4885)

Cat. No.: HY-B1232 Purity: 99.84%
Handling Instructions

Metyrapone (Su-4885) is a potent and orally active 11β-hydroxylase inhibitor and an autophagy activator, also inhibits the production of aldosterone. Metyrapone inhibits synthesis of endogenous adrenal corticosteroid, decreases glucocorticoid levels, and also affects behavior and emotion. In addition, Metyrapone increases the efficiency of autophagic process via downregulation of mTOR pathway, and interacts with Pseudomonas putida cytochrome P-450. Metyrapone can be used for researching Cushing's syndrome and depression.

For research use only. We do not sell to patients.

Metyrapone Chemical Structure

Metyrapone Chemical Structure

CAS No. : 54-36-4

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Solution
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 66 In-stock
Estimated Time of Arrival: December 31
Solid
100 mg USD 60 In-stock
Estimated Time of Arrival: December 31
500 mg USD 180 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Metyrapone:

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Description

Metyrapone (Su-4885) is a potent and orally active 11β-hydroxylase inhibitor and an autophagy activator, also inhibits the production of aldosterone. Metyrapone inhibits synthesis of endogenous adrenal corticosteroid, decreases glucocorticoid levels, and also affects behavior and emotion. In addition, Metyrapone increases the efficiency of autophagic process via downregulation of mTOR pathway, and interacts with Pseudomonas putida cytochrome P-450. Metyrapone can be used for researching Cushing's syndrome and depression[1][2][3][4][5].

IC50 & Target

11β-hydroxylase, Aldosterone, CYP450, Autophagy[1][4][5]

In Vitro

Metyrapone (100 μM; 2 h) hyperactivates autophagy in HepG2, and delays the activation of apoptosis at severe endoplasmic reticulum (ER) stress[5].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Metyrapone (25 or 50 mg/kg; SC, single dosage) decreases the stress-induced increase in plasma corticosterone levels, significantly impairs acquisition performance at high dosage, and increases open arm activity at low dosage[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Sprague-Dawley rats (n=179; 270-300g)[1]
Dosage: 25 or 50 mg/kg (in a volume of 2.0 mL/kg)
Administration: SC, single dosage
Result: Dose-dependently decreased the stress-induced increase in plasma corticosterone levels in the water maze test; the high level dose significantly impaired acquisition performance in the water maze and decreased fear-induced immobility; the lower dose increased open arm activity.
Clinical Trial
Molecular Weight

226.27

Formula

C14H14N2O

CAS No.
SMILES

CC(C1=CC=CN=C1)(C)C(C2=CC=CN=C2)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (441.95 mM; Need ultrasonic)

H2O : ≥ 38 mg/mL (167.94 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.4195 mL 22.0975 mL 44.1950 mL
5 mM 0.8839 mL 4.4195 mL 8.8390 mL
10 mM 0.4419 mL 2.2097 mL 4.4195 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (441.95 mM); Clear solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (11.05 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (11.05 mM); Clear solution

  • 4.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (11.05 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.84%

References
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Metyrapone
Cat. No.:
HY-B1232
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