Turbinaric acid
Based on 1 Customer Validation
Turbinaric acid is a kind of secosqualene carboxylic acid. Turbinaric acid exhibits cytotoxicity against mouse melanoma cells and human colon cancer cells. Turbinaric acid can be used as a chemical marker for T. conoides. Turbinaric acid can be used for research on melanoma and colon cancer.
For research use only. We do not sell to patients.
- Purity : 98.06%
- CAS No.: 56882-00-9
- Formula: C27H44O2
- Molecular Weight:400.64
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Chemical Information
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CAS No. 56882-00-9
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Appearance Oil
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Molecular Weight 400.64
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Formula C27H44O2
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Color Colorless to light yellow
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SMILES
C/C(C)=C/CC/C(C)=C/CC/C(C)=C/CC/C=C(C)/CC/C=C(C)/CCC(O)=O
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Structure Classification
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Initial Source
Phycomyces blakesleeanus
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : ≥ 100 mg/mL (249.60 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
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Data Sheet (268 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
[1]. Asari F, et al. Turbinaric acid, a cytotoxic secosqualene carboxylic acid from the brown alga Turbinaria ornata. J Nat Prod. 1989 Sep-Oct;52(5):1167-9. [Content Brief]
[2]. Le Lann K, et al. Isolation of turbinaric acid as a chemomarker of Turbinaria conoides (J. Agardh) Kützing from South Pacific Islands. J Phycol. 2014 Dec;50(6):1048-57. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.4960 mL | 12.4800 mL | 24.9601 mL | 62.4002 mL |
| 5 mM | 0.4992 mL | 2.4960 mL | 4.9920 mL | 12.4800 mL | |
| 10 mM | 0.2496 mL | 1.2480 mL | 2.4960 mL | 6.2400 mL | |
| 15 mM | 0.1664 mL | 0.8320 mL | 1.6640 mL | 4.1600 mL | |
| 20 mM | 0.1248 mL | 0.6240 mL | 1.2480 mL | 3.1200 mL | |
| 25 mM | 0.0998 mL | 0.4992 mL | 0.9984 mL | 2.4960 mL | |
| 30 mM | 0.0832 mL | 0.4160 mL | 0.8320 mL | 2.0800 mL | |
| 40 mM | 0.0624 mL | 0.3120 mL | 0.6240 mL | 1.5600 mL | |
| 50 mM | 0.0499 mL | 0.2496 mL | 0.4992 mL | 1.2480 mL | |
| 60 mM | 0.0416 mL | 0.2080 mL | 0.4160 mL | 1.0400 mL | |
| 80 mM | 0.0312 mL | 0.1560 mL | 0.3120 mL | 0.7800 mL | |
| 100 mM | 0.0250 mL | 0.1248 mL | 0.2496 mL | 0.6240 mL |
Turbinaric acid Related Classifications
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