2. GPCR/G Protein Neuronal Signaling
  3. Adrenergic Receptor
  4. Phenylephrine

Phenylephrine  (Synonyms: (R)-(-)-Phenylephrine; L-Phenylephrine)

Cat. No.: HY-B0769 Purity: 99.98%
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(R)-(-)-Phenylephrine is a selective α1-adrenoceptor agonist primarily used as a decongestant.

For research use only. We do not sell to patients.

CAS No. : 59-42-7

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
500 mg In-stock
1 g   Get quote  
5 g   Get quote  

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Customer Review

Based on 34 publication(s) in Google Scholar

Other Forms of Phenylephrine:

Phenylephrine Related Antibodies

Top Publications Citing Use of Products

34 Publications Citing Use of MCE Phenylephrine

Others
IF
Cell Imaging/Staining
RT-PCR
WB

    Phenylephrine purchased from MedChemExpress. Usage Cited in: Trends Biotechnol. 2025 Apr;43(4):918-945.  [Abstract]

    Cross-sections of samples fabricated change of double- and triple-layered arterial models following Phenylephrine (4 μM) and Acetylcholine treatment.

    Phenylephrine purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Oct 29:S2090-1232(25)00848-3.  [Abstract]

    si-NC or si-FGF21 were infected with adenovirus encoding LC3 for 24 h, then subjected to Phenylephrine (100 μM) stimulation for 24 h. These cells were stained using MitoTracker Red dye, scale bar = 20 µm.

    Phenylephrine purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Oct 29:S2090-1232(25)00848-3.  [Abstract]

    Neonatal rat cardiomyocytes were infected with si-NC or si-PINK1, and then treated with control or 100 μM Phenylephrine for 24 h, followed by vehicle or FGF21 protein. LC3 and PINK1, FGF21 protein levels.

    Phenylephrine purchased from MedChemExpress. Usage Cited in: Mater Des. 2025 Aug.

    AC16 and NRVMs were treated with DMSO or Phenylephrine hydrochloride (PE; 100 μM) for 48 h to induce cardiac hypertrophy. Upper panel: the expression of MYH7, BNP and METTL3 were detected by immunoblotting. Lower panel: The densities of the signals were plotted as the mean ± SD from 3 independent experiments.

    Phenylephrine purchased from MedChemExpress. Usage Cited in: Biochem Pharmacol. 2025 Aug 8;242(Pt 1):117218.  [Abstract]

    RT-qPCR was used to analyze the expression levels of ANP, BNP, β-MHC in H9c2 cells Phenylephrine stimulation.

    Phenylephrine purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Jul 16;15(1):5985.  [Abstract]

    Contraction of isolated aortic rings from indicated groups in response to the Phenylephrine (10 nM-100 μM) treatment.

    Phenylephrine purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2024 Feb 20:212:477-492.  [Abstract]

    Rhodamine phalloidin staining was used to analyze the cytoskeleton of cardiomyocytes (red). Neonatal rat cardiomyocytes were pretreated with the different concentration of BRE or DMSO vehicle for 2 h, and then stimulated with 10 μM Phenylephrine hydrochloride (PE) for 48 h.

    Phenylephrine purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2024 Feb 20:212:477-492.  [Abstract]

    The levels of hypertrophic marker (β-MHC) and fibrosis indice (COL-1) were detected by Western blot analysis in cardiomyocytes. Cells （H9C2 and Neonatal rat cardiomyocytes，NRCMS） were pretreated with the different concentration of BRE or DMSO vehicle for 2 h, and then stimulated with 10 μM Phenylephrine hydrochloride (PE) for 48 h.

    Phenylephrine purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2024 Feb 20:212:477-492.  [Abstract]

    Intracellular and mitochondrial ROS accumulation were observed and quantitative through DCFH-DA and mitoSOX indicators through confocal laser scanning microscopy (green: DCFH-DA, red: mitoSOX, blue: hoechst 33342). Neonatal rat cardiomyocytes (NRCMs) were pretreated with the different concentration of BRE or DMSO vehicle for 2 h, and then stimulated with 10 μM Phenylephrine hydrochloride (PE) for 48 h.

    Phenylephrine purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2024 Feb 20:212:477-492.  [Abstract]

    The expression of MFN-1 and MFN-2 were analyzed by Western blot in NRCM cardiomyocytes. Neonatal rat cardiomyocytes (NRCMs) were pretreated with the different concentration of BRE or DMSO vehicle for 2 h, and then stimulated with 10 μM Phenylephrine hydrochloride (PE) for 48 h.

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    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    (R)-(-)-Phenylephrine is a selective α1-adrenoceptor agonist primarily used as a decongestant.

    IC50 & Target

    α adrenergic receptor

     

    Cellular Effect
    Cell Line Type Value Description References
    CHO-K1 EC50
    0.86 nM
    Compound: Phenylephrine
    Intrinsic activity at human alpha1B adrenergic receptor expressed in CHOK1 cells co-expressing aequorin assessed as calcium mobilization measured for 20 secs in presence of coelenterazine H by luminescence assay
    Intrinsic activity at human alpha1B adrenergic receptor expressed in CHOK1 cells co-expressing aequorin assessed as calcium mobilization measured for 20 secs in presence of coelenterazine H by luminescence assay
    		[PMID: 27658792]
    In Vitro

    (R)-(-)-Phenylephrine is a selective α1-adrenoceptor agonist with pKi values of 5.86, 4.87 and 4.70 for α1D, α1B and α1A receptors respectively[1][2].
    Phenylephrine promotes cardiac fibroblast proliferation. Phenylephrine activates CaN and evokes NFAT3 nuclear translocation. It suggests that the Ca(2+)/CaN/NFAT pathway mediates phenylephrine -induced cardiac fibroblast proliferation, and this pathway might be a possible therapeutic target in cardiac fibrosis[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Phenylephrine Related Antibodies
    In Vivo

    Perfusion of hearts with 100 μM phenylephrine causes a rapid (maximal at 10 min) 12-fold activation of two p38-MAPK isoforms. α1-adrenoceptor agonists such as phenylephrine increase the contractility of the heart. Phenylephrine also activates SAPKs/JNKs in neonatal ventricular myocytes[4].
    Phenylephrine could increase the alveolar fluid clearance in high tidal volume-ventilated rats and accelerate the absorption of pulmonary edema[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    167.21

    Formula

    C9H13NO2
    CAS No.
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    O[C@H](C1=CC(O)=CC=C1)CNC
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (299.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 5 mg/mL (29.90 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 5.9805 mL 29.9025 mL 59.8050 mL
    5 mM 1.1961 mL 5.9805 mL 11.9610 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 5 mg/mL (29.90 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 5 mg/mL (29.90 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 10 mg/mL (59.81 mM); Clear solution; Need ultrasonic and warming

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.98%

    SDS (554 KB)

    COA (245 KB) HNMR (200 KB) RP-HPLC (239 KB)

    Handling Instructions (2659 KB)
    References
    Animal Administration
    [5]

    Rats: A total of 170 male Wistar rats are randomLy allocated into 17 groups (n=10) using random number tables. Short-term (40 minutes) mechanical ventilation with high tidal volume is performed to induce lung injury, impair active Na+ transport and lung liquid clearance in the rats. Unventilated rats serves as controls. To demonstrate the effect of phenylephrine on alveolar fluid clearance, phenylephrine at different concentrations (10, 1, 0.1, 0.01, and 0.001 μM) is injected into the alveolar space of the HVT ventilated rats[5].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 5.9805 mL 29.9025 mL 59.8050 mL 149.5126 mL
    5 mM 1.1961 mL 5.9805 mL 11.9610 mL 29.9025 mL
    10 mM 0.5981 mL 2.9903 mL 5.9805 mL 14.9513 mL
    15 mM 0.3987 mL 1.9935 mL 3.9870 mL 9.9675 mL
    20 mM 0.2990 mL 1.4951 mL 2.9903 mL 7.4756 mL
    25 mM 0.2392 mL 1.1961 mL 2.3922 mL 5.9805 mL
    DMSO 30 mM 0.1994 mL 0.9968 mL 1.9935 mL 4.9838 mL
    40 mM 0.1495 mL 0.7476 mL 1.4951 mL 3.7378 mL
    50 mM 0.1196 mL 0.5981 mL 1.1961 mL 2.9903 mL
    60 mM 0.0997 mL 0.4984 mL 0.9968 mL 2.4919 mL
    80 mM 0.0748 mL 0.3738 mL 0.7476 mL 1.8689 mL
    100 mM 0.0598 mL 0.2990 mL 0.5981 mL 1.4951 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Phenylephrine Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Phenylephrine59-42-7(R)-(-)-Phenylephrine L-PhenylephrineAdrenergic ReceptorBeta ReceptorInhibitorinhibitorinhibit

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