1. GPCR/G Protein
    Neuronal Signaling
    Metabolic Enzyme/Protease
  2. Cannabinoid Receptor
    Endogenous Metabolite
  3. β-Caryophyllene

β-Caryophyllene (Synonyms: (-)-(E)-Caryophyllene; (−)-β-caryophyllene; (−)-trans-Caryophyllene)

Cat. No.: HY-N1415 Purity: >95.0%
Handling Instructions

β-Caryophyllene is a CB2 receptor agonist.

For research use only. We do not sell to patients.

β-Caryophyllene Chemical Structure

β-Caryophyllene Chemical Structure

CAS No. : 87-44-5

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500 mg USD 50 In-stock
Estimated Time of Arrival: December 31
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β-Caryophyllene is a CB2 receptor agonist.

IC50 & Target

Human Endogenous Metabolite


In Vitro

Among the tested cancer cells, β-Caryophyllene demonstrates selective anti-proliferative effect against three cancer cell lines, namely HCT 116 (colon cancer, IC50=19 μM), PANC-1 (pancreatic cancer, IC50=27 μM), and HT29 (colon cancer, IC50=63 μM) cells, whereas β-Caryophyllene exhibits either moderate or poor cytotoxic effects against ME-180, PC3, K562 and MCF-7. Results show that β-Caryophyllene possesses higher selectivity towards the colorectal cancer cells (HCT 116), with selectivity index (SI)=27.9, followed by PANC-1 and HT 29 cells with SI=19.6 and 8, respectively. The apoptotic index estimated for β-Caryophyllene treatment on HCT 116 cells after 24 h treatment is 64±0.04. β-Caryophyllene at 10 μM concentration, causes significant nuclei condensation after 6 h of treatment. β-caryophyllene exhibits a dose and time-dependent inhibitory effect on the motility of HCT 116 cells[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Treatment with β-Caryophyllene at different doses does not show any effects on swimming speed during the test. Oral treatment with β-Caryophyllene ameliorates the rise in β-amyloid deposition in the transgenic mice in a roughly dose-dependent manner, and the two higher doses exhibit almost equal effects in modifying the β-amyloid burden. The number of activated astroglial cells is higher in vehicle-treated mouse brains than in β-Caryophyllene-treated mouse brains with different doses. β-Caryophyllene is effective at reducing the enhancement of the COX-2 protein level found in vehicle-treated APP/PS1 mice[1]. Animals treated with β-Caryophyllene display higher values of object recognition index than their vehicle-treated counterparts [t(14)=4.204, P<0.05]. The total time spent in object exploration during the test trial is not significantly different between β-Caryophyllene-treated and vehicle-treated animals (t(14)=0.5874, P>0.05). Treatment with β-Caryophyllene does not significantly alter these seizure-induced neurochemical changes[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight







C=C1CC/C=C(C)/CC[[email protected]@]2([H])C(C)(C)C[[email protected]]12[H]


Room temperature in continental US; may vary elsewhere.

Pure form -20°C 3 years
4°C 2 years

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility
In Vitro: 

Ethanol : ≥ 176.67 mg/mL (864.55 mM)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

H2O : < 0.1 mg/mL (insoluble)

*"≥" means soluble, but saturation unknown.

Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.8936 mL 24.4678 mL 48.9356 mL
5 mM 0.9787 mL 4.8936 mL 9.7871 mL
10 mM 0.4894 mL 2.4468 mL 4.8936 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% EtOH    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 13.25 mg/mL (64.84 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% EtOH    90% (20% SBE-β-CD in saline)

    Solubility: 13.25 mg/mL (64.84 mM); Suspended solution; Need ultrasonic

  • 3.

    Add each solvent one by one:  10% EtOH    90% corn oil

    Solubility: ≥ 13.25 mg/mL (64.84 mM); Clear solution

*All of the co-solvents are provided by MCE.
Cell Assay

Panel of human cancer cells such as, pancreatic (PANC-1), colorectal (HCT-116 and HT-29), invasive squamous cell carcinoma (ME-180), leukemia (K562), hormone sensitive and invasive breast cancer cell line (MCF-7), and prostatic (PC3) adenocarcinoma cell lines are used. Cells are incubated in a humidified CO2 incubator at 37°C supplied with 5% CO2. Inhibitory effect of β-Caryophyllene on proliferation of the cell lines is tested using the MTT assay. The selectivity index (SI) for the cytotoxicity of β-Caryophyllene is calculated using the ratio of IC50 of β-Caryophyllene on a normal cell line (NIH-3T3) to the IC50 of β-Caryophyllene on cancer cell lines[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration

Male double transgenic APP/PS1 mice and wild-type littermates are used. The mice are group housed (3 to 5 animals/cage) with a 12:12-hour light/dark cycle and ad libitum access to food and water. In this experiment, animals are orally treated by gavage with 16, 48, or 144 mg/kg of β-Caryophyllene every morning for 10 weeks starting at the age of 7 months. All vehicle solutions are used for the respective control animal treatments and the Morris water maze test is performed[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

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β-Caryophyllene(-)-(E)-Caryophyllene (−)-β-caryophyllene (−)-trans-CaryophylleneCannabinoid ReceptorEndogenous MetaboliteInhibitorinhibitorinhibit

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