Cat. No.: HY-N1415

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β-Caryophyllene

(Synonyms: (-)-(E)-Caryophyllene; (−)-β-caryophyllene; (−)-trans-Caryophyllene)

6 Cited Publications

Customer Review

Based on 6 publication(s) in Google Scholar

β-Caryophyllene is a CB2 receptor agonist.

For research use only. We do not sell to patients.

Purity: 99.75%
CAS No.: 87-44-5
Formula: C₁₅H₂₄
Molecular Weight:204.35

Storage:

Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

β-Caryophyllene Chemical Structure

CAS No.: 87-44-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (6)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

β-Caryophyllene is a CB2 receptor agonist.

IC₅₀ & Target

Human Endogenous Metabolite

All Cannabinoid Receptor Isoforms :

All Endogenous Metabolite Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
LLC-MK2	CC₅₀	1715 μM Compound: 8	Cytotoxicity against monkey LLC-MK2 cells after 96 hrs by sulforhodamine B assay Cytotoxicity against monkey LLC-MK2 cells after 96 hrs by sulforhodamine B assay	[PMID: 22440015]
LLC-MK2	EC₅₀	1593 μM Compound: 8	Antitrypanosomal activity against Trypanosoma cruzi Y trymastigotes infected in monkey LLC-MK2 cells after 24 hrs by Brenner counter method Antitrypanosomal activity against Trypanosoma cruzi Y trymastigotes infected in monkey LLC-MK2 cells after 24 hrs by Brenner counter method	[PMID: 22440015]
LLC-MK2	IC₅₀	63.7 μM Compound: 8	Antitrypanosomal activity against Trypanosoma cruzi Y amastigotes infected in monkey LLC-MK2 cells after 96 hrs by Geimsa staining method Antitrypanosomal activity against Trypanosoma cruzi Y amastigotes infected in monkey LLC-MK2 cells after 96 hrs by Geimsa staining method	[PMID: 22440015]

In Vitro

Among the tested cancer cells, β-Caryophyllene demonstrates selective anti-proliferative effect against three cancer cell lines, namely HCT 116 (colon cancer, IC₅₀=19 μM), PANC-1 (pancreatic cancer, IC₅₀=27 μM), and HT29 (colon cancer, IC₅₀=63 μM) cells, whereas β-Caryophyllene exhibits either moderate or poor cytotoxic effects against ME-180, PC3, K562 and MCF-7. Results show that β-Caryophyllene possesses higher selectivity towards the colorectal cancer cells (HCT 116), with selectivity index (SI)=27.9, followed by PANC-1 and HT 29 cells with SI=19.6 and 8, respectively. The apoptotic index estimated for β-Caryophyllene treatment on HCT 116 cells after 24 h treatment is 64±0.04. β-Caryophyllene at 10 μM concentration, causes significant nuclei condensation after 6 h of treatment. β-caryophyllene exhibits a dose and time-dependent inhibitory effect on the motility of HCT 116 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Treatment with β-Caryophyllene at different doses does not show any effects on swimming speed during the test. Oral treatment with β-Caryophyllene ameliorates the rise in β-amyloid deposition in the transgenic mice in a roughly dose-dependent manner, and the two higher doses exhibit almost equal effects in modifying the β-amyloid burden. The number of activated astroglial cells is higher in vehicle-treated mouse brains than in β-Caryophyllene-treated mouse brains with different doses. β-Caryophyllene is effective at reducing the enhancement of the COX-2 protein level found in vehicle-treated APP/PS1 mice^[1]. Animals treated with β-Caryophyllene display higher values of object recognition index than their vehicle-treated counterparts [t(14)=4.204, P<0.05]. The total time spent in object exploration during the test trial is not significantly different between β-Caryophyllene-treated and vehicle-treated animals (t(14)=0.5874, P>0.05). Treatment with β-Caryophyllene does not significantly alter these seizure-induced neurochemical changes^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 87-44-5
Appearance Liquid (Density: 0.902 g/cm³)
Molecular Weight 204.35
Formula C₁₅H₂₄
Color Colorless to light yellow
SMILES

C=C1CC/C=C(C)/CC[C@@]2([H])C(C)(C)C[C@]12[H]
Synonyms

(-)-(E)-Caryophyllene; (−)-β-caryophyllene; (−)-trans-Caryophyllene
Structure Classification
- Terpenoids
- Sesquiterpenes
Initial Source
- Plants
- other families
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (6)

All Histological Imaging/StainingWBIFIn Vivo Efficacy Study

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Journal Impact Factor

Phytomedicine

Patchouli oil and its main component patchouli alcohol inhibit colorectal cancer by activating ectopic olfactory receptor. [Abstract]

2026 Aug:158:158408. PMID: 42287815
Phytomedicine

β-Caryophyllene promotes the survival of random skin flaps by upregulating the PI3K/AKT signaling pathway. [Abstract]

2024 Jul 25:130:155726. PMID: 38815406

β-Caryophyllene purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jul 25:130:155726. [Abstract]

Histopathological images of diseased sections stained with hematoxylin and eosin. Each image was captured using the same magnification treated with β-Caryophyllene (BCP) (204, 306 mg/kg, p.o.).

β-Caryophyllene purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jul 25:130:155726. [Abstract]

Protein expression patterns of PI3K, AKT, p-AKT, Bax, and Bcl-2 in rat flap tissues were detected by western blotting assays treated with β-Caryophyllene (BCP) (204, 306 mg/kg, p.o.).

β-Caryophyllene purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jul 25:130:155726. [Abstract]

Immunofluorescence staining of ICAM-1 and VCAM-1 treated with β-Caryophyllene (BCP) (204, 306 mg/kg, p.o.).

β-Caryophyllene purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jul 25:130:155726. [Abstract]

Comparison of survival area percentages treated with β-Caryophyllene (BCP) (204, 306 mg/kg, p.o.).
J Pharm Anal

2025 Dec 15.
Int J Mol Sci

Intraplantar β-Caryophyllene Alleviates Pain and Inflammation in STZ-Induced Diabetic Peripheral Neuropathy via CB2 Receptor Activation. [Abstract]

2025 May 7;26(9):4430. PMID: 40362667
Neuropharmacology

β-Caryophyllene prevents cognitive impairment in mice following acute sleep deprivation by alleviating hippocampal structural and functional alterations. [Abstract]

2025 Oct 28:283:110752. PMID: 41167414
SSRN

2025 Jun 13.

Solvent & Solubility

In Vitro:

Ethanol : ≥ 176.67 mg/mL (864.55 mM)

DMSO : 25 mg/mL (122.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.8936 mL

24.4678 mL

48.9356 mL

5 mM

0.9787 mL

4.8936 mL

9.7871 mL

10 mM

0.4894 mL

2.4468 mL

4.8936 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 13.25 mg/mL (64.84 mM); Clear solution

This protocol yields a clear solution of ≥ 13.25 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (132.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)

Solubility: 13.25 mg/mL (64.84 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 13.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (132.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% EtOH 90% Corn Oil

Solubility: ≥ 13.25 mg/mL (64.84 mM); Clear solution

This protocol yields a clear solution of ≥ 13.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (132.5 mg/mL) to 900 μL Corn oil, and mix evenly.

Protocol 4

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (12.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 5

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (12.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 6

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (12.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 100 mg/mL (489.36 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocol

Cell Assay^[2]

Panel of human cancer cells such as, pancreatic (PANC-1), colorectal (HCT-116 and HT-29), invasive squamous cell carcinoma (ME-180), leukemia (K562), hormone sensitive and invasive breast cancer cell line (MCF-7), and prostatic (PC3) adenocarcinoma cell lines are used. Cells are incubated in a humidified CO₂ incubator at 37°C supplied with 5% CO₂. Inhibitory effect of β-Caryophyllene on proliferation of the cell lines is tested using the MTT assay. The selectivity index (SI) for the cytotoxicity of β-Caryophyllene is calculated using the ratio of IC₅₀ of β-Caryophyllene on a normal cell line (NIH-3T3) to the IC₅₀ of β-Caryophyllene on cancer cell lines^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[1]

Male double transgenic APP/PS1 mice and wild-type littermates are used. The mice are group housed (3 to 5 animals/cage) with a 12:12-hour light/dark cycle and ad libitum access to food and water. In this experiment, animals are orally treated by gavage with 16, 48, or 144 mg/kg of β-Caryophyllene every morning for 10 weeks starting at the age of 7 months. All vehicle solutions are used for the respective control animal treatments and the Morris water maze test is performed^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (283 KB)
SDS (557 KB)
- English - EN (557 KB)
- Français - FR (557 KB)
- Deutsch - DE (557 KB)
- Norwegian - NO (557 KB)
- Español - ES (557 KB)
- Swedish - SV (557 KB)
- Italian - IT (557 KB)
- Korean - KR (557 KB)
- Portuguese - PT (557 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Cheng Y, et al. β-Caryophyllene ameliorates the Alzheimer-like phenotype in APP/PS1 Mice through CB2 receptor activation and the PPARγ pathway. Pharmacology. 2014;94(1-2):1-12. [Content Brief]

[2]. Dahham SS, et al. The Anticancer, Antioxidant and Antimicrobial Properties of the Sesquiterpene β-Caryophyllenefrom the Essential Oil of Aquilaria crassna. Molecules. 2015 Jun 26;20(7):11808-29. [Content Brief]

[3]. de Oliveira CC, et al. Anticonvulsant activity of β-caryophyllene against pentylenetetrazol-induced seizures. Epilepsy Behav. 2016 Mar;56:26-31. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / Ethanol	1 mM	4.8936 mL	24.4678 mL	48.9356 mL	122.3391 mL
	5 mM	0.9787 mL	4.8936 mL	9.7871 mL	24.4678 mL
	10 mM	0.4894 mL	2.4468 mL	4.8936 mL	12.2339 mL
	15 mM	0.3262 mL	1.6312 mL	3.2624 mL	8.1559 mL
	20 mM	0.2447 mL	1.2234 mL	2.4468 mL	6.1170 mL
	25 mM	0.1957 mL	0.9787 mL	1.9574 mL	4.8936 mL
	30 mM	0.1631 mL	0.8156 mL	1.6312 mL	4.0780 mL
	40 mM	0.1223 mL	0.6117 mL	1.2234 mL	3.0585 mL
	50 mM	0.0979 mL	0.4894 mL	0.9787 mL	2.4468 mL
	60 mM	0.0816 mL	0.4078 mL	0.8156 mL	2.0390 mL
	80 mM	0.0612 mL	0.3058 mL	0.6117 mL	1.5292 mL
	100 mM	0.0489 mL	0.2447 mL	0.4894 mL	1.2234 mL

Keywords

β-Caryophyllene

6 Cited Publications

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β-Caryophyllene

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Publications Citing Use of MedChemExpress (MCE) β-Caryophyllene

All Cannabinoid Receptor Isoforms

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (6)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

β-Caryophyllene Related Classifications

HY-N1415 Related Classifications