2. Anti-infection
  3. Antibiotic Beta-lactamase Bacterial Penicillin-binding protein (PBP)
  4. AM-112

AM-112 is a β-lactamase (β-lactamase) inhibitor and antibacterial agent, with IC50 values ranging from 0.0002 μg/mL to 0.67 μg/mL against class A, C, and D β-lactamase. By inhibiting PBP2, the penicillin-binding protein of E. coli, and protecting Ceftazidime (HY-B0593) from enzymatic hydrolysis, AM-112 significantly enhances the antibacterial efficacy of Ceftazidime against Gram-negative bacteria, enterococci, and staphylococci. AM-112 exhibits favorable pharmacokinetic properties and acid-base stability. AM-112 can be used for the research of bacterial infections.

For research use only. We do not sell to patients.

AM-112

AM-112 Chemical Structure

CAS No. : 414858-51-8

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

AM-112 Related Antibodies

Top Publications Citing Use of Products

View All Antibiotic Isoform Specific Products:

View All Isoforms
Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

View All Beta-lactamase Isoform Specific Products:

View All Isoforms
Metallo-β-lactamase (MBL) Serine β-lactamase (SBL)

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

AM-112 is a β-lactamase (β-lactamase) inhibitor and antibacterial agent, with IC50 values ranging from 0.0002 μg/mL to 0.67 μg/mL against class A, C, and D β-lactamase. By inhibiting PBP2, the penicillin-binding protein of E. coli, and protecting Ceftazidime (HY-B0593) from enzymatic hydrolysis, AM-112 significantly enhances the antibacterial efficacy of Ceftazidime against Gram-negative bacteria, enterococci, and staphylococci. AM-112 exhibits favorable pharmacokinetic properties and acid-base stability. AM-112 can be used for the research of bacterial infections[1][2].

IC50 & Target

β-lactam

 

In Vitro

AM-112 (combined with Ceftazidime at a 2:1 ratio) synergistically reduces the MIC of Ceftazidime (HY-B0593) in all tested Enterococcus faecalis strains, with the greatest MIC reduction observed in E. faecalis SFZ (from 64 μg/mL to 8 μg/mL)[1].
AM-112 (0.5-32 μg/ml; 18-24 h) has an MIC of 2 μg/mL against Methicillin (HY-121544)-susceptible Staphylococcus aureus, an MIC range of 4-16 μg/mL against anaerobic bacteria of the genera Clostridium and Bacteroides, and an MIC of ≥32 μg/mL against Escherichia coli, Pseudomonas, Acinetobacter, Enterococcus and Methicillin-resistant Staphylococcus aureus[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

AM-112 Related Antibodies

Cell Viability AssayWestern Blot AnalysisCell Proliferation AssayApoptosis AnalysisCell Cytotoxicity AssayCell Cycle AnalysisRT-PCRCell Autophagy AssayImmunofluorescenceCell Differentiation AssayCell Invasion AssayCell Migration Assay Real Time qPCRELISA Assay[1]

Cell Line: Multiple bacterial strains including Escherichia coli, Enterobacter cloacae, Enterococcus faecalis, Pseudomonas aeruginosa ATCC 27853 producing Class A or Class C β-lactamases
Concentration: 10 μg of AM-112 per disk, combined with 30 μg Ceftazidime per disk
Incubation Time: overnight incubation at 37℃
Result: Enhanced the zone diameter of Ceftazidime against bacterial isolates producing Class A and Class C β-lactamases, while it showed no enhancing effect on the activity of Ceftazidime against Pseudomonas aeruginosa ATCC 27853.
Parmacokinetics
Species Dose Route Serum Concentration
Mice[1] 50 mg/kg s.c. 26 mg/L
Mice[1] 50 mg/kg p.o. 0 mg/L
In Vivo

AM-112 (administered subcutaneously at 1-2.6 mg/kg) exhibits potent inhibitory activity against Staphylococcus aureus-induced sepsis in mice, with an ED50 of 2.6 mg/kg, and enhances the efficacy of Ceftazidime (HY-B0593) when used in combination[1].
AM-112 (administered intravenously at 1.9-19 mg/kg) exhibits inhibitory effects against E. cloacae P99-induced sepsis in mice, with an ED50 of 19 mg/kg, and significantly enhances the efficacy of Ceftazidime when co-administered[1].
AM-112 (>40 mg/kg; s.c.) shows limited inhibitory effect on K. pneumoniae SHV-5-induced sepsis in mice, with an ED50 >40 mg/kg, but it can enhance the efficacy of Ceftazidime when administered in combination[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: ICR mice (male, 20-22 g, intraperitoneal inoculation with S. aureus 3816)[1]
Dosage: 2.6 mg/kg (alone); 1.2 mg/kg (4:1 CAZ:AM-112 combination); 1 mg/kg (7:1 CAZ:AM-112 combination)
Administration: subcutaneous injection
Result: Had an ED50 of 2.6 mg/kg (95% CI: 2.2-3.1 mg/kg).
Had an ED50 of 1.2 mg/kg (95% CI for co-administered ceftazidime: 4.0-5.8 mg/kg) in 4:1 CAZ:AM-112 combination.
Had an ED50 of 1 mg/kg (95% CI for co-administered ceftazidime: 6.6-9.7 mg/kg) in 7:1 CAZ:AM-112 combination.
Animal Model: CD1 mice (male, 20-22 g, intraperitoneal inoculation with E. cloacae P99)[1]
Dosage: 19 mg/kg (alone); 2 mg/kg (1:1 CAZ:AM-112 combination); 1.9 mg/kg (2:1 CAZ:AM-112 combination); 2.9 mg/kg (4:1 CAZ:AM-112 combination)
Administration: intravenous injection
Result: Had an ED50 of 19 mg/kg (95% CI: 11.6-33.4 mg/kg).
Had an ED50 of 2 mg/kg (95% CI for co-administered ceftazidime: 1.0-5.5 mg/kg) in 1:1 CAZ:AM-112 combination.
Had an ED50 of 1.9 mg/kg (95% CI for co-administered ceftazidime: 2.3-5.9 mg/kg) in 2:1 CAZ:AM-112 combination.
Had an ED50 of 2.9 mg/kg (95% CI for co-administered ceftazidime: 7.2-17.4 mg/kg) in 4:1 CAZ:AM-112 combination.
Animal Model: ICR mice (female, 20-22 g, intraperitoneal inoculation with K. pneumoniae SHV-5)[1]
Dosage: >40 mg/kg (alone); 33.6 mg/kg (1:1 CAZ:AM-112 combination); 11.9 mg/kg (2:1 CAZ:AM-112 combination)
Administration: subcutaneous injection
Result: Had an ED50 of >40 mg/kg (no 95% CI determined).
Had an ED50 of 33.6 mg/kg (95% CI for co-administered ceftazidime: 25.1-45.1 mg/kg) in 1:1 CAZ:AM-112 combination.
Had an ED50 of 11.9 mg/kg (95% CI for co-administered ceftazidime: 18.8-30.1 mg/kg) in 2:1 CAZ:AM-112 combination.
Molecular Weight

298.34

Formula

C14H22N2O5
CAS No.
SMILES

[C@H](C)(O)[C@@]1([C@@]2(N(C(C(O)=O)=C(C(CCCN)(C)C)O2)C1=O)[H])[H]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

AM-112 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AM-112414858-51-8AM112AM 112AntibioticBeta-lactamaseBacterialPenicillin-binding protein (PBP)β-lactamaseClass D β-lactamasesβ-lactamase inhibitorClass C β-lactamasesClass A β-lactamasesEscherichia coliStaphylococcus aureuspenicillin-binding protein 2bacterial infectionEnterococcus faecalisGram-negative bacteriaInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
AM-112
Cat. No.:
HY-165460
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.