Amorphin 

Amorphin is a phosphorylase b with glycogen metabolism activity. Amorphin binds directly to alpha-actinin, with alpha-actinin mediating its binding to actin filaments. Amorphin can be used for the research of McArdle’s disease and breast cancer^[1][2].

Amorphin (Compound 7) (68 h) selectively inhibits the proliferation of MCF-7 human breast cancer cells with an IC₅₀ of 0.95 μM, and has no effect on HCT-116 human colorectal cancer cells at concentrations up to 50 μM^[1].
Amorphin (1 μg/mL; 2 h at 4°C) directly binds to biotinylated alpha-actinin in a solid-phase dot blot assay^[2].
Amorphin (0.31-20 μg/mL; 15 min) binds with higher affinity to purified alpha-actinin than to purified F-actin in a solid-phase radioimmune assay^[2].
Amorphin (0.5 mg/mL; 5 min) binds to actin filaments only when they are decorated with purified alpha-actinin, as observed via negative stain electron microscopy^[2].
Amorphin binds specifically to the Z-bands of extracted glycerinated adult chicken pectoralis muscle myofibrils, restoring their electron-dense amorphous structure^[2].
Amorphin partial peptide sequencing confirms it is nearly identical to rabbit phosphorylase b, demonstrating amorphin is a phosphorylase b^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

704.68

C₃₄H₄₀O₁₆

4207-90-3

O=C1C=2C(=C3C(O[C@@H](C(CO[C@@H]4O[C@H](CO[C@H]5[C@H](O)[C@@H](O)[C@@H](O)CO5)[C@@H](O)[C@H](O)[C@H]4O)=C)C3)=CC2)O[C@]6([C@@]1(C=7C(OC6)=CC(OC)=C(OC)C7)[H])[H]

Room temperature in continental US; may vary elsewhere.

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• [1]. Wu X, et al. Cytotoxic rotenoid glycosides from the seeds of Amorpha fruticosa. Fitoterapia. 2015;100:75-80.  [Content Brief]

  [2]. Chowrashi P, et al. Amorphin is phosphorylase; phosphorylase is an alpha-actinin-binding protein. Cell Motil Cytoskeleton. 2002;53(2):125-135.  [Content Brief]