CD8-α Antibody (YA9217)
(Synonyms: CD8, CD8 antigen, alpha chain, CD8α, Ly 2, Ly 35)Based on 1 Customer Validation
CD8-α Antibody (YA9217) is a Rat-derived and non-conjugated IgG2a monoclonal antibody, targeting to CD8-α.
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Host:
Rat
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Isotype:
IgG2a
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Application:
IP, ICC/IF, IF-Tissue, IHC-P, FC
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Reactivity :
mouse
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Formulation:
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IP
IP: Immunoprecipitation
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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|---|---|---|---|---|---|
| Dilution Ratio | 1-2μg per 100-500μg Total protein | 1:50-500 | 1:50-500 | 1:100-500 | 1μg per 1*106 cells |
Product Details
CD8-α Antibody (YA9217) is a Rat-derived and non-conjugated IgG2a monoclonal antibody, targeting to CD8-α.
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Host Rat
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Species Reactivitymouse
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Calculated Molecular Weight Predicted band size: 27 kDa
A synthesized peptide derived from mouse CD8-α.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG2a
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at 2-8°C for 1 year, do not freeze.
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Shipping
Shipping with blue ice.
Background
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Function
CD8-α is an Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells
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Subcellular Localization
Cell membrane
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Isoforms & Post-Translational Modification
CD8-α has 1 isoforms, P01731-1: amino acid length is 247, molecular weight is 27456 Da (predicted).
Palmitoylated, but association with CD8B seems to be more important for the enrichment of CdD8A in lipid rafts -
Subunit
Forms disulfide-linked heterodimers with CD8B at the cell surface.
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SwissProt ID
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Synonyms
CD8, CD8 antigen, alpha chain, CD8α, Ly 2, Ly 35
Documentation
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