GPR56 Antibody (YA4598)
(Synonyms: BFPP; BPPR; ADGRG1; TM7LN4; TM7XN1)GPR56 Antibody (YA4598) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to GPR56.
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Host:
Mouse
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Isotype:
IgG1
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Application:
IHC-P, FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
GPR56 Antibody (YA4598) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to GPR56.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 55-70 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 78 kDa
Purified recombinant fragment of human GPR56 (AA: extra(26-225)) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS with 0.05% sodium azide
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
GPR56 is an Adhesion G-protein coupled receptor (aGPCR) for steroid hormone 17alpha-hydroxypregnenolone (17-OH), which is involved in cell adhesion and cell-cell interactions. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as RhoA pathway. ADGRG1 is coupled to G(12) and/or G(13) G proteins (GNA12 and GNA13, respectively) and mediates the activation Rho small GTPases. Acts as a potent suppressor of ferroptosis: binding to 17-OH-binding initiates signaling that down-regulates CD36 and alleviates ferroptosis-induced liver injury. Ligand-binding also induces cell adhesion activity via association with proteins such as collagen III/COL3A1 and TGM2. Mediates cell matrix adhesion in developing neurons and hematopoietic stem cells. Involved in cortical development, specifically in maintenance of the pial basement membrane integrity and in cortical lamination: association with COL3A1 in the developing brain inhibits neuronal migration via activation of the RhoA pathway. Together with TGM2, acts as a regulator of myelination and myelin repair in oligodendrocyte precursor cells. Acts as a hemostatic sensor of shear force: G protein-coupled receptor signaling is activated in response to shear force in platelets, promoting G(13) G protein signaling, and platelet shape change and aggregation in a COL3A1-dependent manner. Acts as an inhibitor of VEGFA production thereby inhibiting angiogenesis through a signaling pathway mediated by PRKCA. Plays a role in the maintenance of hematopoietic stem cells in bone marrow niche. Plays an essential role in testis development[1][2][3][4][5][6][7][8][9][10].
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Subcellular Localization
Cell membrane; Multi-pass membrane protein; Secreted; Membrane raft
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Expression
Tissue_specificity:It is widely distributed, with the highest concentrations in the thyroid, brain, and heart. It is expressed in a large number of tumor cells. Its expression is downregulated in highly metastatic tumor cells. -
Isoforms & Post-Translational Modification
Q9Y653 has 5 isomers: Q9Y653-1: 77738 Da (predicted); Q9Y653-2: 77072 Da (predicted); Q9Y653-3: 77476 Da (predicted); Q9Y653-4: 58655 Da (predicted); Q9Y653-5: 58042 Da (predicted).
Autoproteolytically cleaved into 2 fragments; the large extracellular N-terminal fragment (ADGRG1 NT) and the membrane-bound C-terminal fragment (ADGRG1 CT) predominantly remain associated and non-covalently linked (PubMed:22333914, PubMed:37673336). Shedding to yield the secreted ADGRG1 N-terminal fragment seems to involve metalloprotease(s) (PubMed:22333914);N-glycosylated. Contains sialic acid residues;Ubiquitinated. Undergoes polyubiquitination upon activation -
Subunit
Heterodimer of 2 chains generated by proteolytic processing; the large extracellular N-terminal fragment (ADGRG1 NT) and the membrane-bound C-terminal fragment (ADGRG1-CT) predominantly remain associated and non-covalently linked (PubMed:22333914). ADGRG1 NT self-associates in a trans-trans manner; the homophilic interaction enhances receptor signaling (PubMed:21708946). Interacts with TGM2 (PubMed:16757564, PubMed:21349848, PubMed:21724588). Interacts with heparin; leading to the reduction of ADGRG1 shedding (PubMed:27068534). Interacts with COL3A1 (PubMed:28258187). Part of a GPCR-tetraspanin complex at least consisting of ADGRG1, CD81, eventually CD9, and GNA11 in which CD81 is enhancing the association of ADGRG1 with GNA11 (PubMed:15004227)
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SwissProt ID
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Synonyms
BFPP; BPPR; ADGRG1; TM7LN4; TM7XN1
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