1. Cell Cycle/DNA Damage
    Apoptosis
  2. DNA Alkylator/Crosslinker
    Apoptosis
  3. Busulfan

Busulfan 

Cat. No.: HY-B0245 Purity: ≥98.0%
Handling Instructions

Busulfan is a potent alkylator with selective immunosuppressive effect on bone marrow.

For research use only. We do not sell to patients.

Busulfan Chemical Structure

Busulfan Chemical Structure

CAS No. : 55-98-1

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10 mM * 1 mL in DMSO USD 55 In-stock
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5 g USD 180 In-stock
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Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of Busulfan:

Top Publications Citing Use of Products
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Description

Busulfan is a potent alkylator with selective immunosuppressive effect on bone marrow.

IC50 & Target

DNA Alkylator[1]

In Vitro

Busulfan is a potent alkylating agent, and used as a chemotherapeutic agent. Busulfan (120 μM) results in premature senescence in WI38 cells via the Erk and p38 MAPK pathway, reduces GSH and increases ROS production, but the production can be suppressed by NADPH oxidase[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Busulfan (40 mg/kg, i.p) increases apoptosis and decreases the testis weight in mice. The testes of Busulfan-treated mice exhibits higher level of pRB expression, inhibits Rb phosphorylation and PCNA expression compared to the control[2]. Busulfan (2.5, 5.0 mg/kg, i.p.) causes earlier occurrence of persistent esturs in a dose dependent manner in rats. Busulfan (5.0 mg/kg) also increases the incidence of uterine adenocarcinomas and multiplicity of uterine neoplastic lesions. In addition, Busulfan decreases the serum 17β-estradiol(E2), progesterone and inhibin levels in rats, and elevates the E2/progesterone ratio only at 5.0 mg/kg[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial
Molecular Weight

246.30

Formula

C₆H₁₄O₆S₂

CAS No.
SMILES

CS(=O)(OCCCCOS(C)(=O)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (203.00 mM; Need ultrasonic)

H2O : 1 mg/mL (4.06 mM; ultrasonic and warming and heat to 80°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.0601 mL 20.3004 mL 40.6009 mL
5 mM 0.8120 mL 4.0601 mL 8.1202 mL
10 mM 0.4060 mL 2.0300 mL 4.0601 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (10.15 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (10.15 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (10.15 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay
[1]

WI38 cells are seeded in wells of a 24-well plate at 60,000 cells/well and cultured overnight. They are treated with Busulfan for 24 h, after which the drug is removed and the cells are cultured in fresh medium for up to 11 d. Untreated cells are used as control. Every 3 d the cells are trypsinized, counted and sub-cultured in a fresh medium at 1:5 and 1:2 dilutions for control untreated and Busulfan-treated cells, respectively[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[2]

Mice[2]
One hundred twenty ICR male mice ranging in age from 8 to 12 weeks (30-40 g) are used. Eight-week-old ICR male mice receive a single intraperitoneal injection of Busulfan (40 mg/kg) diluted in sesame oil. The mice are housed in wire cages at 22 ± 1°C under a 12-h light-dark cycle with 70% humidity and fed ad libitum[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: ≥98.0%

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Keywords:

BusulfanDNA Alkylator/CrosslinkerApoptosisInhibitorinhibitorinhibit

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