Butyrolactone 3 (Synonyms: MB-3)

Name: Butyrolactone 3
Brand: MedChemExpress
SKU: HY-129039
Rating: 5.0 (11 reviews)

Cat. No.: HY-129039 Purity: 99.58%: Data Sheet
COA

SDS
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Butyrolactone 3 (MB-3) is a specifical small-molecule inhibitor of the histone acetyltransferase Gcn5 (IC₅₀=100 μM), which has a high affinity to the Gcn5 enzyme comparable to that of its natural substrate, histone H3. Butyrolactone 3 shows weak inhibitory on CBP (IC₅₀=0.5 mM). Butyrolactone 3 can be used in studies of cancer, metabolic, autoimmune and neurological diseases.

For research use only. We do not sell to patients.

CAS No. : 778649-18-6

Size	Stock	Quantity
Free Sample (0.1 - 0.2 mg)	Apply Now
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 11 publication(s) in Google Scholar

11 Publications Citing Use of MCE Butyrolactone 3

Bio/Physico-chemical Assay

Histological Imaging/Staining

: Butyrolactone 3 purchased from MedChemExpress. Usage Cited in: Immunotargets Ther. 2025 Dec 28:14:1515-1529. [Abstract]; HE staining image of r renal tissues in sham-treated and I/R-treated C57BL/6 mice in the presence or absence of Butyrolactone 3 (10 mg/kg, iv).

: Butyrolactone 3 purchased from MedChemExpress. Usage Cited in: Immunotargets Ther. 2025 Dec 28:14:1515-1529. [Abstract]; PAS staining image of kidney from sham-treated and I/R-treated C57BL/6 mice in the presence or absence of Butyrolactone 3 (10 mg/kg, iv).

: Butyrolactone 3 purchased from MedChemExpress. Usage Cited in: Immunotargets Ther. 2025 Dec 28:14:1515-1529. [Abstract]; Masson’s trichrome staining in the renal cortex from sham-treated and I/R-treated C57BL/6 mice in the presence or absence of Butyrolactone 3 (10 mg/kg, iv)

: Butyrolactone 3 purchased from MedChemExpress. Usage Cited in: Immunotargets Ther. 2025 Dec 28:14:1515-1529. [Abstract]; The expression of KAT2A and F4/80 proteins in kidney tissue of IR-AKI mice was up-regulated by uIRI treatment, and significantly decreased after Butyrolactone 3 (10 mg/kg, iv) treatment.

: Butyrolactone 3 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Jul 9:e2406333. [Abstract]; H1299 cells were treated with Lactate (20 mM), A-485, KAT8-IN-1 (300 µM), NU9056 (2 µM), Butyrolactone 3 (MB-3, 300 µM), or Gln-AMS (10 µM) for 14 h. Quantitative analysis of extracellular FFAs was conducted post-treatment.

: Butyrolactone 3 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Apr 30;13(4):421. [Abstract]; Effects of GCN5 inhibitor Butyrolactone 3 on binding of TAK1 with TAB1 and TAB2 in NRCMs under PE treatment.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

GCN5

100 μM (IC₅₀)

In Vitro

Butyrolactone 3 (50, 100, 200 μM, 16 h) can reduce acetylation of Gcn5 and induce degradation of E2A-PBX1^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:	RCH-ACV
Concentration:	50, 100, 200 μM
Incubation Time:	16 h
Result:	Decreased the protein levels of E2A-PBX1 and E2A in a dose-dependent manner. Reduced Wnt16 and GCN5 protein levels.

Molecular Weight

184.19

Formula

C₉H₁₂O₄

CAS No.

778649-18-6

Appearance

Solid

Color

White to off-white

SMILES

O=C([C@@H](C1=C)[C@@H](CCC)OC1=O)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (542.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.4292 mL	27.1459 mL	54.2918 mL
5 mM	1.0858 mL	5.4292 mL	10.8584 mL
10 mM	0.5429 mL	2.7146 mL	5.4292 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (11.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (11.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (11.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*The compound is unstable in solutions, freshly prepared is recommended.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.58%

Select Batch:

Data Sheet (275 KB) SDS (392 KB)

COA (250 KB) HNMR (213 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Biel M,et al. Design, synthesis, and biological evaluation of a small-molecule inhibitor of the histone acetyltransferase Gcn5.Angew Chem Int Ed Engl. 2004 Jul 26;43(30):3974-6 [Content Brief]

[2]. Haque ME, et al. The GCN5: its biological functions and therapeutic potentials. Clin Sci (Lond). 2021 Jan 15;135(1):231-257. [Content Brief]

[3]. Holmlund T, et al. GCN5 acetylates and regulates the stability of the oncoprotein E2A-PBX1 in acute lymphoblastic leukemia. Leukemia. 2013 Mar;27(3):578-85. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.4292 mL	27.1459 mL	54.2918 mL	135.7294 mL
	5 mM	1.0858 mL	5.4292 mL	10.8584 mL	27.1459 mL
	10 mM	0.5429 mL	2.7146 mL	5.4292 mL	13.5729 mL
	15 mM	0.3619 mL	1.8097 mL	3.6195 mL	9.0486 mL
	20 mM	0.2715 mL	1.3573 mL	2.7146 mL	6.7865 mL
	25 mM	0.2172 mL	1.0858 mL	2.1717 mL	5.4292 mL
	30 mM	0.1810 mL	0.9049 mL	1.8097 mL	4.5243 mL
	40 mM	0.1357 mL	0.6786 mL	1.3573 mL	3.3932 mL
	50 mM	0.1086 mL	0.5429 mL	1.0858 mL	2.7146 mL
	60 mM	0.0905 mL	0.4524 mL	0.9049 mL	2.2622 mL
	80 mM	0.0679 mL	0.3393 mL	0.6786 mL	1.6966 mL
	100 mM	0.0543 mL	0.2715 mL	0.5429 mL	1.3573 mL

Butyrolactone 3 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Butyrolactone 3778649-18-6MB-3Butyrolactone3Butyrolactone-3MB3MB 3Histone AcetyltransferaseHATsHATGcn5Histone acetyltransferaseRCH-ACVInhibitorinhibitorinhibit

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Butyrolactone 3 (Synonyms: MB-3)

Customer Review

Butyrolactone 3 Related Antibodies

11 Publications Citing Use of MCE Butyrolactone 3

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Histone Acetyltransferase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Butyrolactone 3 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Butyrolactone 3 Related Classifications

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Your Recently Viewed Products:

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