MG 149 (Synonyms: Tip60 HAT inhibitor)

Name: MG 149
Brand: MedChemExpress
SKU: HY-15887
Rating: 5.0 (13 reviews)

Cat. No.: HY-15887 Purity: 99.60%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

MG149 (Tip60 HAT inhibitor) is a selective and potent Tip60 inhibitor with IC₅₀ of 74 uM, similar potentcy for MOF (IC₅₀ = 47 uM); little potent for PCAF and p300 (IC₅₀ >200 uM). MG 149 inhibits KAT8 and blocks PINK1 kinase activity. MG149 inhibits the phosphorylation of Parkin and ubiquitin, thereby suppressing the initiation of PINK1-dependent mitophagy. MG 149 can reverse chronic restraint stress (CRS) induced hypertension and related molecular changes. MG 149 commonly used in research on diseases such as hypertension and Parkinson's disease.

For research use only. We do not sell to patients.

CAS No. : 1243583-85-8

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 13 publication(s) in Google Scholar

13 Publications Citing Use of MCE MG 149

RT-PCR

Cell Imaging/Staining

: MG 149 purchased from MedChemExpress. Usage Cited in: Microbiol Res. 2025 Dec 5:305:128414. [Abstract]; HEK293 cells transfected with Tip60-Flag were infected with PAO1 for 3 h in the presence or absence of 5 μM MG149, followed by immunoprecipitation of Tip60 and analysis of its HAT activity towards H2A.Z by an in vitro HAT activity assay.

: MG 149 purchased from MedChemExpress. Usage Cited in: Microbiol Res. 2025 Dec 5:305:128414. [Abstract]; Tip60 activity was pharmacologically inhibited using 5 μM MG149 in A549 cells infected with PAO1 for 3 h. Western blot analysis was conducted to evaluate γH2AX expression. The relative abundance of protein expression was quantified using ImageJ software.

: MG 149 purchased from MedChemExpress. Usage Cited in: Microbiol Res. 2025 Dec 5:305:128414. [Abstract]; Tip60 activity was pharmacologically inhibited using 5 μM MG149 in A549 cells infected with PAO1 for 3 h. Representative immunofluorescence images of γH2AX foci formation in A549 cells under the indicated treatment conditions. AF647 (red) was used to label γH2AX, and DAPI (blue) to stain nuclei.

: MG 149 purchased from MedChemExpress. Usage Cited in: Microbiol Res. 2025 Dec 5:305:128414. [Abstract]; A549 cells were infected with PAO1 for 3 h and subsequently maintained in DMEM containing 10 % FBS and gentamicin with continuous treatment of 5 μM MG149 for 24 h. The expression level of γH2AX was examined using Western blot analysis and quantified using ImageJ.

: MG 149 purchased from MedChemExpress. Usage Cited in: Commun Biol. 2025 Aug 30;8(1):1314. [Abstract]; The expression of Vp28 (Left) and Ie1 (Right) in the hemocytes and intestinal tissues of MG149-treated shrimp was detected at 24 hpi via qPCR. Ef-1α was used as the endogenous control gene.

: MG 149 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2023 Mar 3;24(5):4912.; Effect of the KAT inhibitor MG 149 (1 µM; 24 h) on the levels of H4K16 acetylation in A549 cells. The effect was determined by immunofluorescence. The quantification is shown to the right.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

CBP/p300

TIP60

PINK1

In Vitro

MG 149 (Tip60 HAT inhibitor), at 200 μM, inhibited about 90% of Tip60 activity but had no inhibitory impact on p300 and PCAF. MG 149 was essentially competitive with Ac-CoA and noncompetitive with the histone substrate. HAT inhibition studies with MG 149 demonstrated that both compounds inhibited the HAT activity of the nuclear extracts of different regions significantly (p < 0.05)^[1].
MG 149 (100 μM, 3 h) inhibits the activation of the PINK1/Parkin pathway in POE SH-SY5Y cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:	POE SH-SY5Y cells
Concentration:	100 μM
Incubation Time:	3 h
Result:	Significantly reduced pParkin protein levels.

In Vivo

MG 149 (1.6 mg/kg, i.p., once daily, for 14 days) can reverse chronic restraint stress (CRS) induced hypertension in male C57BL/6J mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	CRS induced male C57BL/6J mice ^[3]
Dosage:	1.6 mg/kg
Administration:	Intraperitoneal injection (i.p.), once daily, for 14 days
Result:	Blocked the release of glutamate in brain regions. Downregulated the expression of NR2B and IL-18. Alleviated sympathetic nervous system activation.

Molecular Weight

340.46

Formula

C₂₂H₂₈O₃

CAS No.

1243583-85-8

Appearance

Solid

Color

White to off-white

SMILES

O=C(O)C1=C(O)C=CC=C1CCC2=CC=C(CCCCCCC)C=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (293.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9372 mL	14.6860 mL	29.3720 mL
5 mM	0.5874 mL	2.9372 mL	5.8744 mL
10 mM	0.2937 mL	1.4686 mL	2.9372 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.34 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.34 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.60%

Select Batch:

Data Sheet (275 KB) SDS (251 KB)

COA (244 KB) HNMR (258 KB) RP-HPLC (253 KB) LCMS (336 KB)

Handling Instructions (2659 KB)

References

[1]. Ghizzoni M, et al. 6-alkylsalicylates are selective Tip60 inhibitors and target the acetyl-CoA binding site. Eur J Med Chem. 2012 Jan;47(1):337-44. [Content Brief]

[2]. de Talhouët C, et al. KAT8 compound inhibition inhibits the initial steps of PINK1-dependant mitophagy. Sci Rep. 2024 May 22;14(1):11721. [Content Brief]

[3]. Wang Y, et al. TIP60 mediates stress-induced hypertension via promoting glutamatedmPFC-to-vCA1 release. Clin Exp Hypertens. 2023 Dec 31;45(1):2259130. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9372 mL	14.6860 mL	29.3720 mL	73.4301 mL
	5 mM	0.5874 mL	2.9372 mL	5.8744 mL	14.6860 mL
	10 mM	0.2937 mL	1.4686 mL	2.9372 mL	7.3430 mL
	15 mM	0.1958 mL	0.9791 mL	1.9581 mL	4.8953 mL
	20 mM	0.1469 mL	0.7343 mL	1.4686 mL	3.6715 mL
	25 mM	0.1175 mL	0.5874 mL	1.1749 mL	2.9372 mL
	30 mM	0.0979 mL	0.4895 mL	0.9791 mL	2.4477 mL
	40 mM	0.0734 mL	0.3672 mL	0.7343 mL	1.8358 mL
	50 mM	0.0587 mL	0.2937 mL	0.5874 mL	1.4686 mL
	60 mM	0.0490 mL	0.2448 mL	0.4895 mL	1.2238 mL
	80 mM	0.0367 mL	0.1836 mL	0.3672 mL	0.9179 mL
	100 mM	0.0294 mL	0.1469 mL	0.2937 mL	0.7343 mL

MG 149 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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MG 149 (Synonyms: Tip60 HAT inhibitor)

Customer Review

MG 149 Related Antibodies

13 Publications Citing Use of MCE MG 149

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

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View All Histone Acetyltransferase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

MG 149 Related Antibodies

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Complete Stock Solution Preparation Table

MG 149 Related Classifications

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