1. Epigenetics
    Apoptosis
  2. Histone Acetyltransferase
    Apoptosis
  3. WM-3835

WM-3835 

Cat. No.: HY-134901 Purity: 98.10%
Handling Instructions

WM-3835 is a potent and high-specific HBO1 (KAT7 or MYST2) inhibitor and binds directly to the acetyl-CoA binding site of HBO1 33. WM-3835 activates apoptosis while inhibits osteosarcoma (OS) cell proliferation, migration and invasion. WM-3835 has antitumor activity and potently inhibits pOS-1 xenograft growth in mice.

For research use only. We do not sell to patients.

WM-3835 Chemical Structure

WM-3835 Chemical Structure

CAS No. : 2229025-70-9

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Free Sample (0.5-1 mg)   Apply Now  
10 mM * 1 mL in DMSO USD 110 In-stock
Estimated Time of Arrival: December 31
5 mg USD 100 In-stock
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10 mg USD 170 In-stock
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25 mg USD 350 In-stock
Estimated Time of Arrival: December 31
50 mg USD 550 In-stock
Estimated Time of Arrival: December 31
100 mg USD 950 In-stock
Estimated Time of Arrival: December 31
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500 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

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Description

WM-3835 is a potent and high-specific HBO1 (KAT7 or MYST2) inhibitor and binds directly to the acetyl-CoA binding site of HBO1 33. WM-3835 activates apoptosis while inhibits osteosarcoma (OS) cell proliferation, migration and invasion. WM-3835 has antitumor activity and potently inhibits pOS-1 xenograft growth in mice[1].

In Vitro

WM-3835 (1-25 uM; 24-96 hours) inhibits pOS-1 cell viability in a concentration-dependent manner[1].
WM-3835 (5 uM; 72 h) activates cell apoptosis and significantly increases TUNEL-positive nuclei in pOS-1 cells[1].
WM-3835 (5 µM; 24 hours) downregulates MYLK-HOXA9 mRNA expression in pOS-1 cells[1].
WM-3835 (1-25 uM) suppresses H4K12ac-H3K14ac in a dose-dependent manner. WM-3835 does not alter the expressions of HBO1 protein and total H3, H4 histones[1].
WM-3835 (5 µM) fails to induce apoptosis and reduction of viability in HBO1-KO pOS-1 cells, koHBO1-1 and koHBO1-2, HBO1-low human osteoblasts[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: Primary human OS (pOS-1) cells
Concentration: 1, 5, 10, 25 uM
Incubation Time: 24, 48, 72, 96 hours
Result: Inhibited pOS-1 cell viability in a concentration-dependent manner.
Exerted a significant anti-survival activity at least 48 h, displaying a time-dependent manner.

Apoptosis Analysis[1]

Cell Line: pOS-1 cells
Concentration: 5 uM
Incubation Time: 72 hours
Result: Activated cell apoptosis and significantly increases TUNEL-positive nuclei.

RT-PCR[1]

Cell Line: pOS-1 cells
Concentration: 5 uM
Incubation Time: 24 hours
Result: Downregulated MYLK-HOXA9 mRNA expression.
In Vivo

WM-3835 (10 mg/kg/day; ip; for21 days) potently inhibits pOS-1 xenograft growth in SCID mice[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: SCID mice (18-19 g, female) with pOS1 cells[1]
Dosage: 10 mg/kg
Administration: IP; daily; for 21 days
Result: Potently inhibited pOS-1 xenograft growth.
Molecular Weight

400.42

Formula

C₂₀H₁₇FN₂O₄S

CAS No.
SMILES

O=C(C1=CC(C2=CC=CC=C2)=CC(C)=C1F)NNS(=O)(C3=CC=CC(O)=C3)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (624.34 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4974 mL 12.4869 mL 24.9738 mL
5 mM 0.4995 mL 2.4974 mL 4.9948 mL
10 mM 0.2497 mL 1.2487 mL 2.4974 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (5.19 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (5.19 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (5.19 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

WM-3835WM3835WM 3835Histone AcetyltransferaseApoptosisHATsHATHBO1KAT7MYST2acetyl-CoAosteosarcomaOS) cell proliferationmigrationinvasionpOS-1MYLK-HOXA9H4K12acH3K14acInhibitorinhibitorinhibit

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