NU9056

Name: NU9056
Brand: MedChemExpress
SKU: HY-110127
Rating: 5.0 (6 reviews)

Cat. No.: HY-110127 Purity: 96.62%: Data Sheet
COA

SDS
Handling Instructions
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NU9056 is a potent and selective Tip60 (KAT5) histone acetyltransferase inhibitor with an of 2 µM. NU9056 shows >16-fold selectivity for Tip60 over PCAF, p300 and GCN5. NU9056 induces apoptosis of prostate cancer cells.

For research use only. We do not sell to patients.

CAS No. : 1450644-28-6

Size	Stock	Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Liquid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 6 publication(s) in Google Scholar

6 Publications Citing Use of MCE NU9056

RT-PCR

Bio/Physico-chemical Assay

: NU9056 purchased from MedChemExpress. Usage Cited in: Fish Shellfish Immunol. 2025 Oct 16:168:110946. [Abstract]; The healthy grass carp were separately intraperitoneally injected with 21 μl of NU9056 at a concentration of 20 mM or the same amount of DMSO. After 4 days, the tissues of intestines, liver, spleen, and kidney were separately extracted. The mRNA levels of bax, bcl2, and caspase3 were assessed by qRT-PCR.

: NU9056 purchased from MedChemExpress. Usage Cited in: Fish Shellfish Immunol. 2025 Oct 16:168:110946. [Abstract]; The healthy grass carp were separately intraperitoneally injected with 21 μl of NU9056 at a concentration of 20 mM or the same amount of DMSO. After 4 days, the tissues of intestines, liver, spleen, and kidney were separately extracted. Caspase3 protein levels were analyzed by Western-blot.

: NU9056 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Jul 9:e2406333. [Abstract]; H1299 cells were treated with lactate, A‐485, KAT8‐IN‐1, NU9056 (2 µM), Butyrolactone 3 (MB‐3), or Gln‐AMS for 14 h. Quantitative analysis of extracellular FFAs was conducted post‐treatment.

: NU9056 purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2024 Jun 14:597:217005. [Abstract]; Treatment with the Tip60 inhibitor NU9056 (20 μM for 18 h) reduced USP26 acetylation. Input and anti-HA immunoprecipitates from HEK293T cells transfected with or without Flag-Tip60 were analyzed by WB.

: NU9056 purchased from MedChemExpress. Usage Cited in: Sens Actuators B Chem. 2024 Aug 1, 412, 135827.; The effects of adding NU9056 (0-500 μM) to the Tip60 reaction system were analyzed for fluorescence readout and for UV–vis absorbance readout.

: NU9056 purchased from MedChemExpress. Usage Cited in: Sens Actuators B Chem. 2024 Aug 1, 412, 135827.; Kinetic plots displaying the corresponding relative activity based on fluorescence readout and UV–vis absorbance readout are presented, plotted against the logarithm of NU9056 (0-50 μM) concentrations.

: NU9056 purchased from MedChemExpress. Usage Cited in: Nature. 2023 Oct;622(7981):173-179. [Abstract]; Representative western blots of two biological replicates of HEK293T histones treated with A-485 and NU9056 (10 μM for 24 hours).

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Biological Activity
Purity & Documentation
References
Customer Review

Description

NU9056 is a potent and selective Tip60 (KAT5) histone acetyltransferase inhibitor with an ^[1]^[2] of 2 µM. NU9056 shows >16-fold selectivity for Tip60 over PCAF, p300 and GCN5. NU9056 induces apoptosis of prostate cancer cells^[1].

In Vitro

NU9056 (17-36 μM; 24-96 hours) results in both caspase 3 and caspase 9 activation in a time- and concentration-dependent manner^[1].
? NU9056 (2.5-40 μM; 2 hours) treatment results in decreased levels of acetylated histone H4K16, H3K14 and H4K8, targets for Tip60-mediated acetylation^[1].
? NU9056 treatment also decreases androgen receptor, prostate specific antigen, p53 and p21 protein levels^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:	LNCaP cells
Concentration:	17 µM, 24 µM, 36 µM
Incubation Time:	24 hours, 48 hours, 72 hours, 96 hours
Result:	Induced apoptosis via activation of caspase 3 and caspase 9 in a concentration- and time-dependent manner.

Western Blot Analysis^[1]

Cell Line:	LNCaP cells
Concentration:	2.5 µM, 5 µM, 10 µM, 20 µM, 40 µM
Incubation Time:	2 hours
Result:	Resulted in decreased levels of acetylated histone H4K16, H3K14 and H4K8, targets for Tip60-mediated acetylation.

In Vivo

The mice are injected with Nu9056 (2 μg/g) and the hippocampus is collected 1 h later. Tip60 inhibition reduces H2A.Z binding at the -1 nucleosome of Arc, and the +1 nucleosome of Arc and Syp. Additionally, Nu9056 increases acetylation at the -1 nucleosome of Fos, Tacstd2, and Gria4, and the +1 nucleosome of Gria4^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

232.37

Formula

C₆H₄N₂S₄

CAS No.

1450644-28-6

Appearance

Liquid (Density: 1.60±0.1 g/cm³)

Color

Yellow to brown

SMILES

C1(SSC2=CC=NS2)=CC=NS1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 125 mg/mL (537.94 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.3035 mL	21.5174 mL	43.0348 mL
5 mM	0.8607 mL	4.3035 mL	8.6070 mL
10 mM	0.4303 mL	2.1517 mL	4.3035 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Concentration _(final)

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.81%

Select Batch:

Data Sheet (276 KB) SDS (252 KB)

COA (250 KB) HNMR (231 KB) LCMS (271 KB)

Handling Instructions (2659 KB)

References

[1]. Kelly Coffey, et al. Characterisation of a Tip60 specific inhibitor, NU9056, in prostate cancer. PLoS One. 2012;7(10):e45539. [Content Brief]

[2]. Klotilda Narkaj, et al. Blocking H2A.Z Incorporation via Tip60 Inhibition Promotes Systems Consolidation of Fear Memory in Mice. eNeuro. 2018 Nov 8;5(5):ENEURO.0378-18.2018. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.3035 mL	21.5174 mL	43.0348 mL	107.5870 mL
	5 mM	0.8607 mL	4.3035 mL	8.6070 mL	21.5174 mL
	10 mM	0.4303 mL	2.1517 mL	4.3035 mL	10.7587 mL
	15 mM	0.2869 mL	1.4345 mL	2.8690 mL	7.1725 mL
	20 mM	0.2152 mL	1.0759 mL	2.1517 mL	5.3794 mL
	25 mM	0.1721 mL	0.8607 mL	1.7214 mL	4.3035 mL
	30 mM	0.1434 mL	0.7172 mL	1.4345 mL	3.5862 mL
	40 mM	0.1076 mL	0.5379 mL	1.0759 mL	2.6897 mL
	50 mM	0.0861 mL	0.4303 mL	0.8607 mL	2.1517 mL
	60 mM	0.0717 mL	0.3586 mL	0.7172 mL	1.7931 mL
	80 mM	0.0538 mL	0.2690 mL	0.5379 mL	1.3448 mL
	100 mM	0.0430 mL	0.2152 mL	0.4303 mL	1.0759 mL

NU9056 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NU90561450644-28-6NU 9056NU-9056Histone AcetyltransferaseApoptosisHATsHATTip60KAT5apoptosisprostatecanceracetylationInhibitorinhibitorinhibit

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NU9056

Customer Review

NU9056 Related Antibodies

6 Publications Citing Use of MCE NU9056

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Histone Acetyltransferase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

NU9056 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

NU9056 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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SAFETY DATA SHEET (SDS)

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