Surfactin C1
Based on 1 Customer Validation
Surfactin C1 is an amphiphilic biosurfactants. Surfactin C1 inhibits leukemic cell (HL-60) adhesion to human umbilical vein endothelial cells (HUVEC). Surfactin C1 inhibits adhesion melecules expression, such as ICAM-1 and VCAM-1.
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- Pureté: 99.31%
- CAS No.: 24730-31-2
- Formule: C53H93N7O13
- Masse moléculaire:1036.34
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
Surfactin C1 (0.3-10 μg/mL; 24 h) inhibits the adhesion of leukemic cell and monocyte cell to HUVEC, stimulated by LPS[1].
Surfactin C1 (3 μg/mL; 1 h) inhibits LPS-induced expression of adhesion molecules[1].
Surfactin C1 (3 μg/mL; 1 h) inhibits the interaction of lipid A/LBP[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HUVEC
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Concentration:0.3 μg/mL, 1 μg/mL, 3 μg/mL, 10 μg/mL
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Incubation Time:2 hr and another 4 hr with 1 μg/mL LPS
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Result:Decreased the protein level of ICAM-1 and VCAM-1. Completely inhibited the expression at 3μg/mL with no effect on E-selectin.
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Cell Line:HL-60, THP-1, Jurkat cells
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Concentration:0.3 μg/mL, 1 μg/mL, 3 μg/mL, 10 μg/mL, and 100 μg/mL
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Incubation Time:24 hr accompanied with LPS for viability assay;
2 hr and another 4 hr with LPS for adhesion assay -
Result:Didn’t inhibit cell viability. Inhibited cell adhesion to HUVEC with IC50s of 1.10 μg/mL, 1.45 μg/mL, and 1.43 μg/mL, respectively.
Chemical Information
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CAS No. 24730-31-2
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Appearance Solid
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Masse moléculaire 1036.34
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Formule C53H93N7O13
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Color White to off-white
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
DMSO : 100 mg/mL (96.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.41 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (2.41 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 0.9649 mL | 4.8247 mL | 9.6493 mL | 24.1234 mL |
| 5 mM | 0.1930 mL | 0.9649 mL | 1.9299 mL | 4.8247 mL | |
| 10 mM | 0.0965 mL | 0.4825 mL | 0.9649 mL | 2.4123 mL | |
| 15 mM | 0.0643 mL | 0.3216 mL | 0.6433 mL | 1.6082 mL | |
| 20 mM | 0.0482 mL | 0.2412 mL | 0.4825 mL | 1.2062 mL | |
| 25 mM | 0.0386 mL | 0.1930 mL | 0.3860 mL | 0.9649 mL | |
| 30 mM | 0.0322 mL | 0.1608 mL | 0.3216 mL | 0.8041 mL | |
| 40 mM | 0.0241 mL | 0.1206 mL | 0.2412 mL | 0.6031 mL | |
| 50 mM | 0.0193 mL | 0.0965 mL | 0.1930 mL | 0.4825 mL | |
| 60 mM | 0.0161 mL | 0.0804 mL | 0.1608 mL | 0.4021 mL | |
| 80 mM | 0.0121 mL | 0.0603 mL | 0.1206 mL | 0.3015 mL |
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