1308789-62-9
Chemical Structure
BG-PEG-NH2
Synonym(s): SNAP-PEG-NH2
- CAS No.: 1308789-62-9
- Formula:C22H32N8O5
- Molecular Weight:488.54
InChIKey: AMXSINWJQXAFOW-UHFFFAOYSA-N
SMILES: NCCOCCOCCOCCNC(NCC1=CC=C(C=C1)COC2=NC(N)=NC3=C2N=CN3)=O
Biological Activity: BG-PEG-NH2 (SNAP-PEG-NH2) is an amino-terminal fluorescent probe building block and a precursor for site-specific labeling of SNAP-tag fusion proteins. BG-PEG-NH2 undergoes one-step coupling with activated carboxyl esters to form customized SNAP-tag substrates, which covalently bind to SNAP tags via a thioether bond formed at the Cys145 site. After coupling with a caged carboxyfluorescein, BG-PEG-NH2 forms a probe with Ex/Em = 500/524 nm following ultraviolet uncaging. Coupling of BG-PEG-NH2 with SeTau-647-NHS enables visualization of GPCR signal transduction. BG-PEG-NH2 is suitable for coupling reactions in aqueous phases or buffers, conjugation of labels, and labeling of intracellular or cell-surface proteins[1][2][3].
| Cat. No. | Product Name | Purity | Description | Pricing | |||||||||||||||||||
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BG-PEG-NH2 | 99.02% | BG-PEG-NH2 (SNAP-PEG-NH2) is an amino-terminal fluorescent probe building block and a precursor for site-specific labeling of SNAP-tag fusion proteins. BG-PEG-NH2 undergoes one-step coupling with activated carboxyl esters to form customized SNAP-tag substrates, which covalently bind to SNAP tags via a thioether bond formed at the Cys145 site. After coupling with a caged carboxyfluorescein, BG-PEG-NH2 forms a probe with Ex/Em = 500/524 nm following ultraviolet uncaging. Coupling of BG-PEG-NH2 with SeTau-647-NHS enables visualization of GPCR signal transduction. BG-PEG-NH2 is suitable for coupling reactions in aqueous phases or buffers, conjugation of labels, and labeling of intracellular or cell-surface proteins. | ||||||||||||||||||||
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References
- [1]. Corrêa IR, et al. Considerations and protocols for the synthesis of custom protein labeling probes. Methods in molecular biology (Clifton, N.J.). 2015;1266:55-79. [Content Brief]
- [2]. Campos C, et al. Labelling cell structures and tracking cell lineage in zebrafish using SNAP-tag. Developmental dynamics : an official publication of the American Association of Anatomists. 2011 Apr;240(4):820-7.
- [3]. Nishiguchi T, et al. Synergetic Roles of Formyl Peptide Receptor 1 Oligomerization in Ligand-Induced Signal Transduction. ACS chemical biology. 2020 Sep 18;15(9):2577-2587.
