Cefatrizine
Cefatrizine (BL-S-640) is an orally active and broad-spectrum cephalosporin antibiotic. Cefatrizine is also a eEF2K inhibitor, with anti-proliferative activity in human breast cancer cells, which could induce ER stress, leading to cell death. Cefatrizine can be used in studies of cancer and bacterial infection.
For research use only. We do not sell to patients.
- CAS No.: 51627-14-6
- Formula: C18H18N6O5S2
- Molecular Weight:462.50
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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β-lactam |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MDA-MB-436 | IC50 |
30 μM
Compound: 40
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Anticancer activity against human MDA-MB-436 cells assessed as cell growth inhibition incubated for 24 hrs by MTT assay
Anticancer activity against human MDA-MB-436 cells assessed as cell growth inhibition incubated for 24 hrs by MTT assay
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[PMID: 33650861] |
Cefatrizine (0-100 µM; 24 h) causes a remarkable anti-proliferative effect on MCF-7 and MDA-MB-436 cell growth in a dose-dependent manner[1].
Cefatrizine (30 µM; 12 h) induces ER stress in breast cancer cells[1].
Cefatrizine (12, 24, 36 h) increases level of CHOP (marker of ER stress induced apoptosis) and promotes expressions of core proteins in eEF2K-modulated ER stress pathways (Bip, p-PERK, XBP-1S and p-JNK) in MCF-7 and MDA-MB-436 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MCF-7, MDA-MB-436 cells
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Concentration:0-100 µM
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Incubation Time:24 h
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Result:Led to a remarkable anti-proliferative effect on cells and resulted in almost 50% inhibition in the MCF-7 and MDA-MB-436 cells when at 33 µM and 29 µM.
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Cell Line:MCF-7, MDA-MB-436 cells
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Concentration:30 µM
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Incubation Time:12 h
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Result:Led to massive cytoplasmic vacuolization.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:MaleSwiss-Webster mice (19-21 g; P. mirabilis intracystically infected model)[2].
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Dosage:0.2, 1, 5, 25 mg/kg
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Administration:Oral administration; 4 times daily for 3 days.
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Result:Reduced the number of infecting organisms to less than 1,000 in the bladder when 1 mg/kg, and in the kidneys when 0.2 mg/kg.
Chemical Information
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CAS No. 51627-14-6
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Molecular Weight 462.50
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Formula C18H18N6O5S2
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SMILES
O=C(O)C(N1[C@@]([H])([C@@H](C1=O)NC([C@@H](C2=CC=C(C=C2)O)N)=O)SC3)=C3CSC4=CNN=N4
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Synonyms
BL-S-640; SK&F 60771
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
H2O : 250 mg/mL (540.54 mM; Need ultrasonic)
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
References
[1]. Yao Z, et al. Integrative bioinformatics and proteomics-based discovery of an eEF2K inhibitor (cefatrizine) with ER stress modulation in breast cancer cells. Mol Biosyst. 2016 Mar;12(3):729-36. [Content Brief]
[2]. Leitner F, et al. BL-S640, a cephalosporin with a broad spectrum of antibacterial activity: bioavailability and therapeutic properties in rodents. Antimicrob Agents Chemother. 1975 Mar;7(3):306-10. [Content Brief]
Complete Stock Solution Preparation Table
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 2.1622 mL | 10.8108 mL | 21.6216 mL | 54.0541 mL |
| 5 mM | 0.4324 mL | 2.1622 mL | 4.3243 mL | 10.8108 mL | |
| 10 mM | 0.2162 mL | 1.0811 mL | 2.1622 mL | 5.4054 mL | |
| 15 mM | 0.1441 mL | 0.7207 mL | 1.4414 mL | 3.6036 mL | |
| 20 mM | 0.1081 mL | 0.5405 mL | 1.0811 mL | 2.7027 mL | |
| 25 mM | 0.0865 mL | 0.4324 mL | 0.8649 mL | 2.1622 mL | |
| 30 mM | 0.0721 mL | 0.3604 mL | 0.7207 mL | 1.8018 mL | |
| 40 mM | 0.0541 mL | 0.2703 mL | 0.5405 mL | 1.3514 mL | |
| 50 mM | 0.0432 mL | 0.2162 mL | 0.4324 mL | 1.0811 mL | |
| 60 mM | 0.0360 mL | 0.1802 mL | 0.3604 mL | 0.9009 mL | |
| 80 mM | 0.0270 mL | 0.1351 mL | 0.2703 mL | 0.6757 mL | |
| 100 mM | 0.0216 mL | 0.1081 mL | 0.2162 mL | 0.5405 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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