Cefotaxime (Synonyms: Cefotaxim; HR-756)

Name: Cefotaxime
Brand: MedChemExpress
SKU: HY-A0088A
Rating: 5.0 (12 reviews)

Cat. No.: HY-A0088A Purity: 98.74%: Data Sheet
COA

SDS
Handling Instructions
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Cefotaxime, a β-lactamase stable cephalosporin and a third-generation cephalosporin antibiotic, possesses broad-spectrum antibiotic activity against numerous Gram-positive and Gram-negative bacteria.

For research use only. We do not sell to patients.

CAS No. : 63527-52-6

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
250 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 12 publication(s) in Google Scholar

Other Forms of Cefotaxime:

12 Publications Citing Use of MCE Cefotaxime

Microbiological Assay

: Cefotaxime purchased from MedChemExpress. Usage Cited in: AMB Express. 2024 Dec 24;14(1):141. [Abstract]; Combinational antimicrobial effects between PVB and conventional antibiotics against MRSA ATCC 43,300. TET, Tetracycline. DOX, Doxycycline. E, Erythromycin. AZI, Azithromycin. P, Penicillin. CAZ, Ceftazidime. AMP, Ampicillin. CEZ, Cefazolin. CEF, Cefotaxime. OXA, Oxacillin. CRO, Ceftriaxone. AMK, Amikacin. GEN, Gentamycin, KANA, Kanamycin. TOB, Tobramycin. SPC, Spectinomycin.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

β-lactam

Cellular Effect

Cell Line	Type	Value	Description	References
S2	IC₅₀	4.68 mM Compound: Cefotaxime	TP_TRANSPORTER: inhibition of PGF2alpha in OAT2-S2 cells TP_TRANSPORTER: inhibition of PGF2alpha in OAT2-S2 cells	[PMID: 12650826]
S2	IC₅₀	800 μM Compound: Cefotaxime	TP_TRANSPORTER: inhibition of Estrone sulfate uptake in OAT3-expressing S2 cells TP_TRANSPORTER: inhibition of Estrone sulfate uptake in OAT3-expressing S2 cells	[PMID: 12005172]
S2	IC₅₀	> 2000 μM Compound: Cefotaxime	TP_TRANSPORTER: inhibition of PAH uptake in OAT1-expressing S2 cells TP_TRANSPORTER: inhibition of PAH uptake in OAT1-expressing S2 cells	[PMID: 12005172]
S2	IC₅₀	> 5 mM Compound: Cefotaxime	TP_TRANSPORTER: inhibition of PGF2alpha in OAT2-S2 cells TP_TRANSPORTER: inhibition of PGF2alpha in OAT2-S2 cells	[PMID: 12650826]

In Vitro

Cefotaxime exhibits an MIC of 0.0625 mg/L for V. vulnificus CMCP6^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

The combination of ciprofloxacin and cefotaxime is more effective in clearing V. vulnificus in vivo than previously used regimens^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female, specific pathogen free, 8-week-old BALB/c mice^[4].
Dosage:	30 mg/kg.
Administration:	IP every 6 h.
Result:	The viable bacterial counts in liver were lower in mice treated with cefotaxime-plus-ciprofloxacin than in those treated with cefotaxime alone (P<0.001 at 24 h and 48 h, each).

Molecular Weight

455.47

Formula

C₁₆H₁₇N₅O₇S₂

CAS No.

63527-52-6

Appearance

Solid

Color

White to off-white

SMILES

O=C(C(N12)=C(COC(C)=O)CS[C@]2([H])[C@H](NC(/C(C3=CSC(N)=N3)=N\OC)=O)C1=O)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (548.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 100 mg/mL (219.55 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1955 mL	10.9777 mL	21.9553 mL
5 mM	0.4391 mL	2.1955 mL	4.3911 mL
10 mM	0.2196 mL	1.0978 mL	2.1955 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.57 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.57 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.57 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.74%

Select Batch:

Data Sheet (279 KB) SDS (419 KB)

COA (254 KB) HNMR (261 KB) RP-HPLC (228 KB) MS (71 KB)

Handling Instructions (2659 KB)

References

[1]. Hee-Chang Jang, et al. In vivo efficacy of the combination of ciprofloxacin and cefotaxime against Vibrio vulnificus sepsis. PLoS One. 2014 Jun 30;9(6):e101118. [Content Brief]

[2]. Woodfield JC, et al. A comparison of the prophylactic efficacy of ceftriaxone and cefotaxime in abdominal surgery. Am J Surg. 2003 Jan;185(1):45-9. [Content Brief]

[3]. Scholz H, et al. Prospective comparison of ceftriaxone and cefotaxime for the short-term treatment of bacterial meningitis in children. Chemotherapy. 1998 Mar-Apr;44(2):142-7. [Content Brief]

[4]. Gums JG, et al. Differences between ceftriaxone and cefotaxime: microbiological inconsistencies. Ann Pharmacother. 2008 Jan;42(1):71-9. [Content Brief]

[5]. E L Francke, et al. Use of cefotaxime, a beta-lactamase stable cephalosporin, in the therapy of serious infections, including those due to multiresistant organisms. Am J Med. 1981 Sep;71(3):435-42. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.1955 mL	10.9777 mL	21.9553 mL	54.8884 mL
	5 mM	0.4391 mL	2.1955 mL	4.3911 mL	10.9777 mL
	10 mM	0.2196 mL	1.0978 mL	2.1955 mL	5.4888 mL
	15 mM	0.1464 mL	0.7318 mL	1.4637 mL	3.6592 mL
	20 mM	0.1098 mL	0.5489 mL	1.0978 mL	2.7444 mL
	25 mM	0.0878 mL	0.4391 mL	0.8782 mL	2.1955 mL
	30 mM	0.0732 mL	0.3659 mL	0.7318 mL	1.8296 mL
	40 mM	0.0549 mL	0.2744 mL	0.5489 mL	1.3722 mL
	50 mM	0.0439 mL	0.2196 mL	0.4391 mL	1.0978 mL
	60 mM	0.0366 mL	0.1830 mL	0.3659 mL	0.9148 mL
	80 mM	0.0274 mL	0.1372 mL	0.2744 mL	0.6861 mL
	100 mM	0.0220 mL	0.1098 mL	0.2196 mL	0.5489 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Cefotaxime Related Classifications

Inflammation/Immunology Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cefotaxime63527-52-6Cefotaxim HR-756HR756HR 756HR-756Beta-lactamaseBacterialAntibioticβ-lactamaseInhibitorinhibitorinhibit

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Cefotaxime (Synonyms: Cefotaxim; HR-756)

Customer Review

Other Forms of Cefotaxime:

Cefotaxime Related Antibodies

12 Publications Citing Use of MCE Cefotaxime

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Beta-lactamase Isoform Specific Products:

View All Antibiotic Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Cefotaxime Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Cefotaxime Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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