Art. -Nr.: HY-N0218

Benzoylmesaconine

(Synonyms: Mesaconine 14-benzoate)

1 Cited Publications

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Based on 1 publication(s) in Google Scholar

Benzoylmesaconine is a monoester-type alkaloid and is the most abundant component of Wutou decoction, which is widely used in China for rheumatoid arthritis. Benzoylmesaconine exhibits potent anti-inflammatory properties by inhibiting NF-κB. Benzoylmesaconine can suppress NLRP3 inflammasome activation by inhibiting IL-1β secretion and GSDMD-N protein expression. Benzoylmesaconine reduces intracellular K⁺ efflux and disrupts NLRP3 inflammasome assembly.

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Reinheit: 99.56%
CAS. Nr.: 63238-67-5
Formel: C₃₁H₄₃NO₁₀
Molecular Weight:589.67

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Benzoylmesaconine Chemical Structure

CAS. Nr.: 63238-67-5

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Biologische Aktivität
- Beschreibung
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Alle NF-κB Isoform-spezifische Produkte anzeigen :

Alle Interleukin Related Isoform-spezifische Produkte anzeigen :

In Vitro

Benzoylmesaconine (Compound BMA) (0-160 μM) effectively suppresses nigericin-induced IL-1β release in LPS-primed BMDMs in a concentration-dependent manner in bone marrow-derived macrophages (BMDMs)^[3].
Benzoylmesaconine (0-80 μM, 30 min) significantly inhibits the activation of caspase-1 and the secretion of IL-1β and key downstream effectors of the NLRP3 inflammasome in BMDMs^[3].
Benzoylmesaconine (0-80 μM) significantly reduces lactate dehydrogenase release and cell death, suggesting its ability to inhibit pyroptosis^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ELISA Assay^[3]

Cell Line:

(HY-D1056)-primed bone marrow-derived macrophages (BMDMs) stimulated with Nigericin (HY-127019)
Concentration:

0, 20, 40, 80 μM
Incubation Time:

30 min
Result:

Had inhibitory effect on IL-1β release.

In Vivo

Benzoylmesaconine (Compound BMA) (5-20 mg/kg, i.p., single dose) significantly reduces left hindfoot swelling and inflammatory cell infiltration in MSU-induced acute gouty arthritis mice compared to model group^[3].
Benzoylmesaconine (5-20 mg/kg, i.p., single dose) significantly inhibits DSS-induced colitis in mice model^[3].
Benzoylmesaconine (5-20 mg/kg, i.p., single dose) inhibits air pouch inflammation in MSU crystal induced air pouch mouse model^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

MSU-induced acute gouty arthritis mice^[3]
Dosage:

5, 10, 20 mg/kg
Administration:

Intraperitoneal injection (i.p.)
Result:

Suppressed pro-inflammatory cytokine production.
Significantly reduced left hindfoot swelling.
Significantly reduced inflammatory cell infiltration.

Animal Model:

MSU crystal induced air pouch mouse model^[3]
Dosage:

5, 10, 20 mg/kg
Administration:

Intraperitoneal injection (i.p.)
Result:

Effectively inhibited NLRP3 protein expression , caspase-1 cleavage and IL-1β release.
Significantly alleviated mucosal thickening and inflammatory cell infiltration.

Chemical Information

CAS. Nr. 63238-67-5
Appearance Solid
Molecular Weight 589.67
Formel C₃₁H₄₃NO₁₀
Color White to off-white
SMILES

CO[C@@H]1C2([C@@](C[C@@]3(O)[C@@H]4OC(C5=CC=CC=C5)=O)([H])[C@@]4([H])[C@](O)([C@@H](O)[C@@H]3OC)C6C2N(C)C7)[C@@]([C@H]6OC)([H])[C@@]7(COC)[C@H](O)C1
Synonyms

Mesaconine 14-benzoate
Structure Classification
- Alkaloids
- Other Alkaloids
Initial Source
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (1)

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Research Square Preprint

2021 Feb.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (169.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 12.5 mg/mL (21.20 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.6959 mL

8.4793 mL

16.9585 mL

5 mM

0.3392 mL

1.6959 mL

3.3917 mL

10 mM

0.1696 mL

0.8479 mL

1.6959 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.24 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (4.24 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (4.24 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (290 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Dai PM, et al. Pharmacokinetic comparisons of benzoylmesaconine in rats using ultra-performance liquid chromatography-tandem mass spectrometry after administration of pure benzoylmesaconine and Wutou decoction. Molecules. 2014 Oct 17;19(10):16757-69. [Content Brief]

[2]. Ye L, et al. Monoester-Diterpene Aconitum Alkaloid Metabolism in Human Liver Microsomes: Predominant Role of CYP3A4 and CYP3A5. Evid Based Complement Alternat Med. 2013;2013:941093. [Content Brief]

[3]. Zhang, Z., et al., (2024). Benzoylmesaconine mitigates NLRP3 inflammasome-related diseases by reducing intracellular K+ efflux and disrupting NLRP3 inflammasome assembly. Phytomedicine : international journal of phytotherapy and phytopharmacology, 135, 156154. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	1.6959 mL	8.4793 mL	16.9585 mL	42.3963 mL
	5 mM	0.3392 mL	1.6959 mL	3.3917 mL	8.4793 mL
	10 mM	0.1696 mL	0.8479 mL	1.6959 mL	4.2396 mL
	15 mM	0.1131 mL	0.5653 mL	1.1306 mL	2.8264 mL
	20 mM	0.0848 mL	0.4240 mL	0.8479 mL	2.1198 mL
DMSO	25 mM	0.0678 mL	0.3392 mL	0.6783 mL	1.6959 mL
	30 mM	0.0565 mL	0.2826 mL	0.5653 mL	1.4132 mL
	40 mM	0.0424 mL	0.2120 mL	0.4240 mL	1.0599 mL
	50 mM	0.0339 mL	0.1696 mL	0.3392 mL	0.8479 mL
	60 mM	0.0283 mL	0.1413 mL	0.2826 mL	0.7066 mL
	80 mM	0.0212 mL	0.1060 mL	0.2120 mL	0.5300 mL
	100 mM	0.0170 mL	0.0848 mL	0.1696 mL	0.4240 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Benzoylmesaconine

1 Cited Publications

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Benzoylmesaconine

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Publications Citing Use of MedChemExpress (MCE) Benzoylmesaconine

Alle NF-κB Isoform-spezifische Produkte anzeigen

Alle Interleukin Related Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (1)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Benzoylmesaconine Related Classifications

HY-N0218 Related Classifications